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The map label for this gene is ycnB [H]

Identifier: 57652184

GI number: 57652184

Start: 2235375

End: 2236817

Strand: Reverse

Name: ycnB [H]

Synonym: SACOL2157

Alternate gene names: 57652184

Gene position: 2236817-2235375 (Counterclockwise)

Preceding gene: 57652185

Following gene: 57652183

Centisome position: 79.62

GC content: 33.47

Gene sequence:

>1443_bases
ATGGCTAAAGTTGAATTAACAACCCGGAGACGAAACTTCATAGTTGCTGTTATGTTGATTAGTGCGTTTGTAGCTATTTT
AAATCAAACATTATTAAATACAGCGTTACCTAGTATAATGAGAGAATTAAATATCAATGAAAGTACATCGCAATGGCTAG
TTACTGGGTTTATGCTTGTTAATGGCGTCATGATACCTCTGACGGCATATCTAATGGATAGAATTAAAACTAGACCTTTA
TACTTAGCGGCGATGGGGACATTTTTATTAGGTTCTATTGTTGCAGCCTTAGCTCCGAATTTTGGAGTTTTAATGTTAGC
TCGTGTAATTCAAGCGATGGGTGCAGGCGTACTTATGCCCTTAATGCAATTTACGTTATTTACATTGTTCAGTAAAGAAC
ATCGAGGTTTTGCAATGGGACTAGCAGGTTTAGTAATTCAATTTGCACCAGCAATAGGACCTACAGTTACAGGATTAATT
ATTGATCAAGCGAGTTGGCGAGTTCCATTTATTATAATTGTAGGAATTGCTATACTTGCCTTTGTTTTCGGTTTGGTTTC
AATCTCGAGTTACAATGAAGTGAAATATACGAAATTAGATAAGCGTTCAGTAATGTATTCAACTATTGGGTTCGGGTTAA
TGCTATACGCATTTAGTAGTGCAGGAGATTTAGGATTTACAAGTCCAATAGTAATAGGTGCGTTGATATTAAGTATGGTT
ATTATCTATTTATTTATACGTAGACAATTTAATATTACTAATGCACTTTTAAATTTAAGGGTTTTTAAAAATAGAACATT
TGCATTATGTACGATTAGTTCAATGATTATAATGATGTCAATGGTTGGACCTGCGCTGCTTATACCGCTATATGTTCAAA
ACAGTTTATCTTTATCTGCCTTGTTATCAGGACTTGTTATCATGCCTGGTGCAATAATAAATGGTATTATGTCAGTTTTT
ACAGGTAAATTTTATGATAAGTATGGTCCTAGACCATTGATTTATACTGGTTTTACAATTTTAACAATTACTACAATTAT
GTTGTGTTTCTTGCATACAGACACATCTTATACGTATTTAATAGTCGTATATGCAATTAGAATGTTTTCAGTTTCTTTAC
TCATGATGCCGATAAATACTACAGGAATTAATTCTTTGAGAAATGAAGAAATCTCACATGGCACGGCTATTATGAACTTT
GGTCGTGTAATGGCTGGTTCACTAGGCACAGCTTTAATGGTTACATTAATGAGTTTTGGTGCAAAAATATTTTTATCTAC
ATCGCCATCGCATTTAACTGCAACTGAAATTAAACAGCAATCCATTGCTATAGGGGTGGATATCTCATTTGCTTTTGTAG
CTGTGCTTGTTATGGCAGCTTATGTGATAGCACTTTTTATAAGAGAACCTAAAGAAATAGAAAGTAATAGAAGGAAATTT
TAA

Upstream 100 bases:

>100_bases
TGTAGTGCTTCTTTATTATTTGAGTACTACTCCCATTATGTTCATGGCTATACGTATTAAAGGGACAAGTGTATATCTGT
TTTAAAAAGGAGGAATTATG

Downstream 100 bases:

>100_bases
AATAATTATAGTAGTTGGTCTATTTAAAATAATAGGCTAACTGCTTTTTTTATTTAATAAAAAGTTTTATACTTTTAGTG
ATAGACTAAGCAAAAATTGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPL
YLAAMGTFLLGSIVAALAPNFGVLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
IDQASWRVPFIIIVGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALILSMV
IIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMIIMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVF
TGKFYDKYGPRPLIYTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEISHGTAIMNF
GRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISFAFVAVLVMAAYVIALFIREPKEIESNRRKF

Sequences:

>Translated_480_residues
MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPL
YLAAMGTFLLGSIVAALAPNFGVLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
IDQASWRVPFIIIVGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALILSMV
IIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMIIMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVF
TGKFYDKYGPRPLIYTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEISHGTAIMNF
GRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISFAFVAVLVMAAYVIALFIREPKEIESNRRKF
>Mature_479_residues
AKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLY
LAAMGTFLLGSIVAALAPNFGVLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLII
DQASWRVPFIIIVGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALILSMVI
IYLFIRRQFNITNALLNLRVFKNRTFALCTISSMIIMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFT
GKFYDKYGPRPLIYTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEISHGTAIMNFG
RVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISFAFVAVLVMAAYVIALFIREPKEIESNRRKF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=486, Percent_Identity=25.9259259259259, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1789042, Length=407, Percent_Identity=26.2899262899263, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790195, Length=415, Percent_Identity=24.0963855421687, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=24.2352941176471, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788392, Length=489, Percent_Identity=23.3128834355828, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323735, Length=316, Percent_Identity=22.7848101265823, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52639; Mature: 52508

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLV
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
NGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFGVLMLARVIQAMGAGVLMP
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
LMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLIIDQASWRVPFIIIVGIAILA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCHHHHHHHHHHHH
FVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALILSMV
HHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMIIMMSMVGPALLIPLYVQNSLSLSA
HHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLIYTGFTILTITTIMLCFLHTDTSYTYL
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHH
IVVYAIRMFSVSLLMMPINTTGINSLRNEEISHGTAIMNFGRVMAGSLGTALMVTLMSFG
HHHHHHHHHHHHHHEEECCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
AKIFLSTSPSHLTATEIKQQSIAIGVDISFAFVAVLVMAAYVIALFIREPKEIESNRRKF
CEEEEECCCCCCHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
AKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLV
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
NGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFGVLMLARVIQAMGAGVLMP
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
LMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLIIDQASWRVPFIIIVGIAILA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCHHHHHHHHHHHH
FVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALILSMV
HHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMIIMMSMVGPALLIPLYVQNSLSLSA
HHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLIYTGFTILTITTIMLCFLHTDTSYTYL
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHH
IVVYAIRMFSVSLLMMPINTTGINSLRNEEISHGTAIMNFGRVMAGSLGTALMVTLMSFG
HHHHHHHHHHHHHHEEECCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
AKIFLSTSPSHLTATEIKQQSIAIGVDISFAFVAVLVMAAYVIALFIREPKEIESNRRKF
CEEEEECCCCCCHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]