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The map label for this gene is prfA

Identifier: 57652140

GI number: 57652140

Start: 2169855

End: 2170931

Strand: Reverse

Name: prfA

Synonym: SACOL2110

Alternate gene names: 57652140

Gene position: 2170931-2169855 (Counterclockwise)

Preceding gene: 57652141

Following gene: 57652139

Centisome position: 77.27

GC content: 35.19

Gene sequence:

>1077_bases
GTGTTTGATCAATTAGATATTGTAGAAGAAAGATACGAACAGTTAAATGAACTGTTAAGTGACCCAGATGTTGTAAATGA
TTCAGATAAATTACGTAAATATTCTAAAGAGCAAGCTGATTTACAAAAAACTGTAGATGTTTATCGTAACTATAAAGCTA
AAAAAGAAGAATTAGCTGATATTGAAGAAATGTTAAGTGAGACTGATGATAAAGAAGAAGTAGAAATGTTAAAAGAGGAG
AGTAATGGTATTAAAGCTGAACTTCCAAATCTTGAAGAAGAGCTTAAAATATTATTGATTCCTAAAGATCCTAATGATGA
CAAAGACGTTATTGTAGAAATAAGAGCAGCAGCAGGTGGTGATGAGGCTGCGATTTTTGCTGGTGATTTAATGCGTATGT
ATTCAAAGTATGCTGAATCACAAGGATTCAAAACTGAAATAGTAGAAGCGTCTGAAAGTGACCATGGTGGTTACAAAGAA
ATTAGTTTCTCAGTTTCTGGTAATGGCGCGTATAGTAAATTGAAATTTGAAAATGGTGCGCACCGCGTTCAACGTGTGCC
TGAAACAGAATCAGGTGGACGTATTCATACTTCAACAGCTACAGTGGCAGTTTTACCAGAAGTTGAAGATGTAGAAATTG
AAATTAGAAATGAAGATTTAAAAATCGACACGTATCGTTCAAGTGGTGCAGGTGGTCAGCACGTAAACACAACTGACTCT
GCAGTACGTATTACCCATTTACCAACTGGTGTCATTGCAACATCTTCTGAGAAGTCTCAAATTCAAAACCGTGAAAAAGC
AATGAAAGTGTTAAAAGCACGTTTATACGATATGAAAGTTCAAGAAGAACAACAAAAGTATGCGTCACAACGTAAATCAG
CAGTCGGTACTGGTGATCGTTCAGAACGTATTCGAACTTATAATTATCCACAAAGCCGTGTAACAGACCATCGTATAGGT
CTAACGCTTCAAAAATTAGGGCAAATTATGGAAGGCCATTTAGAAGAAATTATAGATGCACTGACTTTATCAGAGCAGAC
AGATAAATTGAAAGAACTTAATAATGGTGAATTATAA

Upstream 100 bases:

>100_bases
TGATCCGATTATCTTAGTAGGTGCCAATGAAAGTTATGAGCCACGTTGTCGCGCGCACCATATCGTAGCACCTAGTGATA
ATAATAAGGAGGAATTATAA

Downstream 100 bases:

>100_bases
AGAAAAGTTAGATGAAGCAATTCATTTAACACAACAAAAAGGGTTTGAACAAACACGAGCTGAATGGTTAATGTTAGATG
TATTTCAATGGACGCGTACG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEE
SNGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIG
LTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL

Sequences:

>Translated_358_residues
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEE
SNGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIG
LTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL
>Mature_358_residues
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEE
SNGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIG
LTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=46.5753424657534, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=37.3937677053824, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI166795305, Length=236, Percent_Identity=41.1016949152542, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=49.7191011235955, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=39.766081871345, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=303, Percent_Identity=36.963696369637, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=333, Percent_Identity=40.5405405405405, Blast_Score=228, Evalue=9e-61,
Organism=Drosophila melanogaster, GI19921226, Length=335, Percent_Identity=40.2985074626866, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_STAA1 (A7X4W4)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442692.1
- ProteinModelPortal:   A7X4W4
- SMR:   A7X4W4
- STRING:   A7X4W4
- EnsemblBacteria:   EBSTAT00000003777
- GeneID:   5560208
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2102
- eggNOG:   COG0216
- GeneTree:   EBGT00050000024287
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   SAUR418127:SAHV_2102-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40350; Mature: 40350

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELAD
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGG
HHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCC
DEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKEISFSVSGNGAYSKLKFENGA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDR
EEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL
CCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELAD
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGG
HHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCC
DEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKEISFSVSGNGAYSKLKFENGA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDR
EEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL
CCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA