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Identifier: 57652048

GI number: 57652048

Start: 1847832

End: 1848680

Strand: Reverse

Name: dat

Synonym: SACOL1800

Alternate gene names: 57652048

Gene position: 1848680-1847832 (Counterclockwise)

Preceding gene: 57652049

Following gene: 57652047

Centisome position: 65.8

GC content: 32.86

Gene sequence:

>849_bases
ATGGAAAAAATTTTTTTAAATGGTGAGTTTGTAAGTCCAAGTGAAGCAAAGGTTTCATACAACGACAGAGGATACGTATT
TGGCGATGGTATTTATGAATACATTCGAGTATATAATGGTAAGTTATTTACAGTAACAGAACATTATGAAAGATTTTTAC
GTAGTGCCAATGAGATTGGTTTAGATTTAAATTATTCTGTAGAAGAATTAATTGAACTATCTCGTAAATTAGTTGATATG
AATCAAATTGAAACTGGGGCAATTTATATTCAAGCAACGCGTGGTGTAGCTGAAAGGAATCATAGCTTCCCGACACCTGA
AGTAGAACCAGCAATTGTTGCTTATACAAAGAGTTATGATCGTCCTTATGATCATTTAGAAAATGGTGTGAATGGTGTTA
CCGTTGAAGATATCCGATGGTTACGTTGCGACATTAAAAGCTTGAACTTATTAGGAAATGTATTAGCAAAAGAATATGCT
GTGAAATATAATGCAGTTGAAGCAATTCAACATCGAGGTGAAACTGTAACTGAAGGATCTTCAAGTAATGCTTATGCAAT
TAAAGACGGTGTGATTTATACACATCCGATTAACAACTATATTCTTAATGGTATTACACGAATTGTAATTAAAAAAATTG
CCGAAGACTATAACATCCCATTTAAAGAAGAAACGTTTACTGTAGATTTCTTGAAAAACGCAGATGAAGTTATTGTTTCA
AGTACTTCAGCTGAGGTTACACCTGTTATTAAATTAGATGGTGAACCAGTTAATGATGGTAAAGTTGGCCCAATTACACG
TCAACTACAAGAAGGATTTGAAAAGTATATAGAGTCACACAGTATTTAA

Upstream 100 bases:

>100_bases
AATGCATCAGAAAAATGAATATATCACTAAAAAACAGTTATTTAACGCAACTAGTATTTACTTAGAAGCAATTTATTCAT
TATGCGTGGAGGAATAATAT

Downstream 100 bases:

>100_bases
AATTCTTTCATCATATTTTTAGATTAAATTACTTAAATTTATCAACTTTCGTAAGTGAATTATGTTATAATTTAAAACGA
AGCTTAAAAGTGTTGTTTAG

Product: D-alanine aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDM
NQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEETFTVDFLKNADEVIVS
STSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKYIESHSI

Sequences:

>Translated_282_residues
MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDM
NQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEETFTVDFLKNADEVIVS
STSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKYIESHSI
>Mature_282_residues
MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDM
NQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEETFTVDFLKNADEVIVS
STSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKYIESHSI

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Escherichia coli, GI48994963, Length=286, Percent_Identity=28.6713286713287, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787338, Length=241, Percent_Identity=24.896265560166, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DAAA_STAAC (Q5HF24)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186633.1
- ProteinModelPortal:   Q5HF24
- SMR:   Q5HF24
- STRING:   Q5HF24
- EnsemblBacteria:   EBSTAT00000010484
- GeneID:   3236054
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1800
- TIGR:   SACOL1800
- eggNOG:   COG0115
- GeneTree:   EBGT00050000025413
- HOGENOM:   HBG617123
- OMA:   RDGWLTE
- ProtClustDB:   CLSK885534
- BioCyc:   SAUR93062:SACOL1800-MONOMER
- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784
- PANTHER:   PTHR11825
- TIGRFAMs:   TIGR01121

Pfam domain/function: PF01063 Aminotran_4; SSF56752 Aminotrans_IV

EC number: =2.6.1.21

Molecular weight: Translated: 31894; Mature: 31894

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: ACT_SITE 146-146 BINDING 32-32 BINDING 51-51 BINDING 99-99 BINDING 101-101 BINDING 178-178 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIG
CCEEEECCCEECCCCCEEEECCCCEEECCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCC
LDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYD
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCCCCCCEEEEEECCCC
RPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYAVKYNAVEAIQHRGETVTEGS
CCHHHHHCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
SSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEETFTVDFLKNADEVIVS
CCCEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCEEEE
STSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKYIESHSI
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIG
CCEEEECCCEECCCCCEEEECCCCEEECCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCC
LDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYD
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCCCCCCEEEEEECCCC
RPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYAVKYNAVEAIQHRGETVTEGS
CCHHHHHCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
SSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEETFTVDFLKNADEVIVS
CCCEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCEEEE
STSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKYIESHSI
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA