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The map label for this gene is truB [H]

Identifier: 57651841

GI number: 57651841

Start: 1303208

End: 1304125

Strand: Direct

Name: truB [H]

Synonym: SACOL1290

Alternate gene names: 57651841

Gene position: 1303208-1304125 (Clockwise)

Preceding gene: 57651840

Following gene: 57651842

Centisome position: 46.39

GC content: 32.35

Gene sequence:

>918_bases
ATGTATAATGGGATATTACCAGTATATAAAGAGCGCGGTTTAACAAGTCATGACGTTGTATTCAAATTGCGTAAAATATT
AAAAACTAAAAAAATAGGTCACACGGGTACGCTTGATCCCGAAGTTGCAGGCGTGTTACCGGTATGTATAGGTAATGCAA
CGAGAGTTAGTGATTATGTTATGGATATGGGCAAAGCTTATGAAGCAACTGTATCGATAGGAAGAAGTACAACGACTGAA
GATCAAACGGGTGATACATTGGAAACAAAAGGTGTACACTCAGCAGATTTTAATAAGGACGATATTGACCGATTGTTAGA
AAGTTTTAAAGGTATCATTGAACAAATTCCGCCGATGTACTCATCCGTCAAAGTAAATGGTAAAAAATTATATGAATATG
CGCGTAATAATGAAACAGTTGAAAGACCAAAGCGTAAAGTTAATATTAAAGACATTGGGCGTATATCTGAATTAGATTTT
AAAGAAAATGAGTGTCATTTTAAAATACGCGTCATCTGTGGTAAAGGTACATATATTAGAACGCTAGCAACTGATATTGG
TGTGAAATTAGGCTTTCCGGCACATATGTCGAAATTAACACGAATCGAGTCTGGTGGATTTGTGTTGAAAGATAGCCTTA
CATTAGAACAAATAAAAGAACTTCATGAGCAGGATTCATTGCAAAATAAATTGTTTCCTTTAGAATATGGATTAAAGGGT
TTGCCAAGCATTAAAATTAAAGATTCGCACATAAAAAAACGTATTTTAAATGGGCAGAAATTTAATAAAAATGAATTTGA
TAACAAAATTAAAGACCAAATTGTATTTATTGATGATGATTCAGAAAAAGTATTAGCAATTTATATGGTACACCCTACAA
AAGAATCAGAAATTAAACCTAAAAAAGTCTTTAATTAA

Upstream 100 bases:

>100_bases
ATACATAGATGTGTAGAAATAGTTAACATTTTCCAGTTTTTTTATGAATAAATTTAGTTGATACGCTATTAAAATATATT
TTAAAAAAGAAGGTGACTAT

Downstream 100 bases:

>100_bases
AGGAGATAGAATTTATGAAAGTCATAGAAGTGACACATCCTATACAATCTAAACAGTATATTACAGAGGATGTTGCAATG
GCATTCGGATTTTTCGATGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTE
DQTGDTLETKGVHSADFNKDDIDRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN

Sequences:

>Translated_305_residues
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTE
DQTGDTLETKGVHSADFNKDDIDRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN
>Mature_305_residues
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTE
DQTGDTLETKGVHSADFNKDDIDRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=260, Percent_Identity=34.2307692307692, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4503337, Length=305, Percent_Identity=28.1967213114754, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI215599015, Length=260, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI2367200, Length=227, Percent_Identity=37.8854625550661, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17553978, Length=257, Percent_Identity=25.2918287937743, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=297, Percent_Identity=28.956228956229, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=139, Percent_Identity=39.568345323741, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281364189, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281364187, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281364185, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281364183, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI62471759, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17975520, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34593; Mature: 34593

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYV
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
MDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDIDRLLESFKGIIEQIPPMY
HHHCCHHHHHEECCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
SSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDFKENECHFKIRVICGKGTYIR
HHEEECHHHHHHHHHCCCHHHHHHHCCCHHHCCCHHCCCCCCCCCEEEEEEEECCCCEEE
TLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
HHHHHHCEECCCHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCC
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKP
CCCEEEEHHHHHHHHHCCCCCCCHHHCCHHHCEEEEEECCCCEEEEEEEECCCCCCCCCC
KKVFN
HHCCC
>Mature Secondary Structure
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYV
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
MDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDIDRLLESFKGIIEQIPPMY
HHHCCHHHHHEECCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
SSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDFKENECHFKIRVICGKGTYIR
HHEEECHHHHHHHHHCCCHHHHHHHCCCHHHCCCHHCCCCCCCCCEEEEEEEECCCCEEE
TLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
HHHHHHCEECCCHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCC
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKP
CCCEEEEHHHHHHHHHCCCCCCCHHHCCHHHCEEEEEECCCCEEEEEEEECCCCCCCCCC
KKVFN
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA