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The map label for this gene is nrdE [H]

Identifier: 57651568

GI number: 57651568

Start: 815735

End: 817840

Strand: Direct

Name: nrdE [H]

Synonym: SACOL0792

Alternate gene names: 57651568

Gene position: 815735-817840 (Clockwise)

Preceding gene: 57651567

Following gene: 57651569

Centisome position: 29.04

GC content: 32.86

Gene sequence:

>2106_bases
ATGAAAACCATGGAAGAGAAAAAGTACAATCATATTGAATTAAATAATGAGGTCACTAAACGAAGAGAAGATGGATTCTT
TAGTTTAGAAAAAGACCAAGAAGCTTTAGTAGCTTATTTAGAAGAAGTAAAAGACAAAACAATCTTCTTCGACACTGAAA
TCGAGCGTTTACGTTATTTAGTAGACAACGATTTTTATTTCAATGTGTTTGATATTTATAGTGAAGCGGATCTAATTGAA
ATCACTGATTATGCAAAATCAATCCCGTTTAATTTTGCAAGTTATATGTCAGCTAGTAAATTTTTCAAAGATTACGCTTT
GAAAACAAATGATAAAAGTCAATACTTAGAAGACTATAATCAACACGTTGCCATTGTTGCTTTATACCTAGCAAATGGTA
ATAAAGCACAAGCTAAACAATTTATTTCTGCTATGGTTGAACAAAGATATCAACCAGCGACACCAACATTTTTAAACGCA
GGCCGTGCGCGTCGTGGTGAGCTAGTGTCATGTTTCTTATTAGAAGTGGATGACAGCTTAAATTCAATTAACTTTATTGA
TTCAACTGCAAAACAATTAAGTAAAATTGGGGGCGGCGTTGCAATTAACTTATCTAAATTGCGTGCACGTGGTGAAGCAA
TTAAAGGAATTAAAGGCGTAGCGAAAGGCGTTTTACCTATTGCTAAGTCACTTGAAGGTGGCTTTAGCTATGCAGATCAA
CTTGGTCAACGCCCTGGTGCTGGTGCTGTGTACTTAAATATCTTCCATTATGATGTAGAAGAATTTTTAGATACTAAAAA
AGTAAATGCGGATGAAGATTTACGTTTATCTACAATATCAACTGGTTTAATTGTTCCATCTAAATTCTTCGATTTAGCTA
AAGAAGGTAAGGACTTTTATATGTTTGCACCTCATACAGTTAAAGAAGAATATGGTGTGACATTAGACGATATCGATTTA
GAAAAATATTATGATGACATGGTTGCAAACCCAAATGTTGAGAAAAAGAAAAAGAATGCGCGTGAAATGTTGAATTTAAT
TGCGCAAACACAATTACAATCAGGTTATCCATATTTAATGTTTAAAGATAATGCTAACAGAGTGCATCCGAATTCAAACA
TTGGACAAATTAAAATGAGTAACTTATGTACGGAAATTTTCCAACTACAAGAAACTTCAATTATTAATGACTATGGTATT
GAAGACGAAATTAAACGTGATATTTCTTGTAACTTGGGCTCATTAAATATTGTTAATGTAATGGAAAGCGGAAAATTCAG
AGATTCAGTTCACTCTGGTATGGACGCATTAACTGTTGTGAGTGATGTAGCAAATATTCAAAATGCACCAGGAGTTAGAA
AAGCTAACAGTGAATTACATTCAGTTGGTCTTGGTGTGATGAATTTACACGGTTACCTAGCAAAAAATAAAATTGGTTAT
GAGTCAGAAGAAGCAAAAGATTTTGCAAATATCTTCTTTATGATGATGAATTTCTACTCAATCGAACGTTCAATGGAAAT
CGCTAAAGAGCGTGGTATCAAATATCAAGACTTTGAAAAGTCTGATTATGCTAATGGCAAATATTTCGAGTTCTATACAA
CTCAAGAATTTGAACCTCAATTCGAAAAAGTACGTGAATTATTCGATGGTATGGCTATTCCTACTTCTGAGGATTGGAAG
AAACTACAACAAGATGTTGAACAATATGGTTTATATCATGCATATAGATTAGCAATTGCTCCAACACAAAGTATTTCTTA
TGTTCAAAATGCAACAAGTTCTGTAATGCCAATCGTTGACCAAATTGAACGTCGTACTTATGGTAATGCGGAAACATTTT
ACCCTATGCCATTCTTATCACCACAAACAATGTGGTACTACAAATCAGCATTCAATACTGATCAGATGAAATTAATCGAT
TTAATTGCGACAATTCAAACGCATATTGACCAAGGTATCTCAACGATCCTTTATGTTAATTCTGAAATTTCTACACGTGA
GTTAGCAAGATTATATGTATATGCGCACTATAAAGGATTAAAATCACTTTACTATACTAGAAATAAATTATTAAGTGTAG
AAGAATGTACAAGTTGTTCTATCTAA

Upstream 100 bases:

>100_bases
ACGATATCAGAAGAGTATAATGTCCCTTTATTAATGAAGTTTGAGTTACATGGAAAAAACAAAGACGTTATTGAATTTAA
GAACAAGGTGGGTAATTTTA

Downstream 100 bases:

>100_bases
CAATTAAATGTTGAAAATGACAAACAGCTAATCATCTGGTCTGAATTAGCAGATGATTAGACTGCTATGTCTGTATTTGT
CAATTATTGAGTAACATTAC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIE
ITDYAKSIPFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPIAKSLEGGFSYADQ
LGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDL
EKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGI
EDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVGLGVMNLHGYLAKNKIGY
ESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWK
KLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLID
LIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI

Sequences:

>Translated_701_residues
MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIE
ITDYAKSIPFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPIAKSLEGGFSYADQ
LGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDL
EKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGI
EDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVGLGVMNLHGYLAKNKIGY
ESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWK
KLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLID
LIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI
>Mature_701_residues
MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIE
ITDYAKSIPFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPIAKSLEGGFSYADQ
LGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDL
EKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGI
EDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVGLGVMNLHGYLAKNKIGY
ESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWK
KLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLID
LIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=635, Percent_Identity=26.9291338582677, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1789030, Length=687, Percent_Identity=41.6302765647744, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI2367133, Length=572, Percent_Identity=23.9510489510489, Blast_Score=152, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17555242, Length=642, Percent_Identity=26.01246105919, Blast_Score=184, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=649, Percent_Identity=25.7318952234206, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6320914, Length=646, Percent_Identity=24.3034055727554, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17352481, Length=631, Percent_Identity=27.2583201267829, Blast_Score=188, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80127; Mature: 80127

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYL
CCCCCCCCCCEEECCCHHHHHHCCCCEEECCHHHHHHHHHHHHCCCEEEECCHHHHHHHH
VDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFFKDYALKTNDKSQYLEDYN
HCCCCEEEEEECCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
QHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSL
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEEEEEECCCC
NSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPIAKSLEGGFSYADQ
CCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFY
HCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEE
MFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLM
EECCCHHHHHHCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGIEDEIKRDISCNLGSLNIVNV
EECCCCEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEH
MESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVGLGVMNLHGYLAKNKIGY
HHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHCCHHHHHHHHHHHCCCCC
ESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHH
FEKVRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEEEEECCCHHHHHHHHHHHHHHHHHH
QIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVN
HHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
SEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYL
CCCCCCCCCCEEECCCHHHHHHCCCCEEECCHHHHHHHHHHHHCCCEEEECCHHHHHHHH
VDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFFKDYALKTNDKSQYLEDYN
HCCCCEEEEEECCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
QHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSL
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEEEEEECCCC
NSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPIAKSLEGGFSYADQ
CCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFY
HCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEE
MFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLM
EECCCHHHHHHCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGIEDEIKRDISCNLGSLNIVNV
EECCCCEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEH
MESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVGLGVMNLHGYLAKNKIGY
HHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHHCCHHHHHHHHHHHCCCCC
ESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHH
FEKVRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEEEEECCCHHHHHHHHHHHHHHHHHH
QIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVN
HHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
SEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]