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The map label for this gene is kipI [H]

Identifier: 57651553

GI number: 57651553

Start: 796518

End: 797228

Strand: Direct

Name: kipI [H]

Synonym: SACOL0776

Alternate gene names: 57651553

Gene position: 796518-797228 (Clockwise)

Preceding gene: 57651552

Following gene: 57651554

Centisome position: 28.35

GC content: 29.96

Gene sequence:

>711_bases
ATGAAAATATATAGTCAAGGTGACCAAGCCATTGTAGTCGCAATTGAAAAAGAAGTATCTAAAAGTTTAACCGAAGATTT
ATTAACACTTCGCTCATATTTAATTGAACAAAATTATCCATTTATTATAGAAATTGTGCCATCAGAATCAGACATGATGA
TTGTCTATGACGCAAGGGATATGATTAAACACCATAATATACAATCACCTTTTTTATACATGAAAGCACTAATAGAATCG
ATTCATTTAAACATAAAACATGATTTTAACCAGCAAGATTTGATTGAAATACCAATTGTGTATGGTTCGAAATATGGTCC
GGATTTAGAATCACTTTTAAAACATTACAAAATCAAGCTAGAAACTTTTATTGAATTACATTCTAAGGCGCAATATTTTG
TTTCGATGATGGGATATTCACCTGGGTTTCCTTATTTAACTGGATTAAATAAGAAATTGTATATTAATCACACGAGTAAA
CAGAAAAAATTCATTCCAGCTGGTTCTGTAGTACTTGAAGGGAAAAAATGCGGTATTGTAACTACGGATACAATTAATGA
TTGGTTAGTTATTGGTTATACACCATTATCACTTTTTAATCCGAAAGAATCAGATTTCGCACGCTTAAAGTTAGGCGATA
ATATTAAATTTAGACCTATCAATGAAAATGAATTAGAAGTAGGAGCGTTTAAAGATGTCAATCATAATTGA

Upstream 100 bases:

>100_bases
GTACCACCAGTAAATTTAGTGAGTAGGTAAAATGAACTATTCAATCGATTCTATAATTAAAAATGAAAGTAAAGTAACCA
AATATTGAAGAGGTGTACAA

Downstream 100 bases:

>100_bases
AAAAAGTGGCTTATTCAGTAGCTTTCAGGACTTTGGCAGAAGGGGATATGAACATGATGGTGTAATTCCATGTGGTGCAC
TTGATACTTTAGCACATGAA

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIEIVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIES
IHLNIKHDFNQQDLIEIPIVYGSKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFRPINENELEVGAFKDVNHN

Sequences:

>Translated_236_residues
MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIEIVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIES
IHLNIKHDFNQQDLIEIPIVYGSKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFRPINENELEVGAFKDVNHN
>Mature_236_residues
MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIEIVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIES
IHLNIKHDFNQQDLIEIPIVYGSKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFRPINENELEVGAFKDVNHN

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=222, Percent_Identity=29.7297297297297, Blast_Score=120, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 27124; Mature: 27124

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIEIVPSESDMMIVYDARD
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCHH
MIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYGSKYGPDLESLLKHYKIKL
HHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHH
ETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSKQKKFIPAGSVVLEGKKCGIV
HHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEECCCCCEECCCCCEEECCCCCCEE
TTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFRPINENELEVGAFKDVNHN
EECCCCCEEEEEEEEEEECCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIEIVPSESDMMIVYDARD
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCHH
MIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYGSKYGPDLESLLKHYKIKL
HHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHH
ETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSKQKKFIPAGSVVLEGKKCGIV
HHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEECCCCCEECCCCCEEECCCCCCEE
TTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFRPINENELEVGAFKDVNHN
EECCCCCEEEEEEEEEEECCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]