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The map label for this gene is queE [H]

Identifier: 57651549

GI number: 57651549

Start: 791665

End: 792378

Strand: Reverse

Name: queE [H]

Synonym: SACOL0770

Alternate gene names: 57651549

Gene position: 792378-791665 (Counterclockwise)

Preceding gene: 57651550

Following gene: 57651548

Centisome position: 28.2

GC content: 36.13

Gene sequence:

>714_bases
ATGGCTAAAATTCCTGTACTCGAAATATTCGGTCCAACCATTCAGGGTGAAGGTCGAGTAATCGGTAGAAAAACGATGTT
TGTGAGAACAGCTGGATGTGATTATCGCTGTAGCTGGTGTGATTCTGCATTTACTTGGGATGGTAGTGCTAAAGGCGATA
TAAAACTCATGACCGCTGAAGAAATTTATGATGAATTAAAACGAATTGGTGGCGATTTATTTAACCACGTTACAATTTCT
GGTGGTAACCCAGCATTAATTAAAGGTATCCAAGAGTTAGTTGACTTATTTCAAGATAAAGGTATTTTCAGCGCACTGGA
GACACAAGGCAGTAAATTCCAACCTTGGATGACACAAATTGATGATTTAACGATTAGTCCAAAACCACCAAGTTCAACTA
TGACACCTGATTTAAAAAAGTTAGATGAAGTGATTACACAATGCGTACCTTCATCATTAAACTTAAAAGTAGTTGTATTC
GACGATAAAGATTATGATTTTGCCAAAATGATACACCACCGTTATCCAGATATTCCCTTCTATTTACAAGTTGGTAACCC
ATATTTATCAGACAGCGTAGATAATCATACCGAAAAGTTGTTAGAACGCTACGAGCAGTTGGTGGATTTAGTTATGCAAA
GTAATGATATGAATCACGTTTATGTGTTACCACAACTTCATACATTACTTTGGAGCAATAAAAAAGGTGTATAA

Upstream 100 bases:

>100_bases
TTTGGCATCGCTCGAACACCAACCAAAATGTATTCAAGTATTTGTAAGAGAAACACCAACAAGTTATGTCGTATTTAGAC
CAAAGGAACAGGTGTAACTA

Downstream 100 bases:

>100_bases
AGGATAACTTATTTTACTTTTTGCAAGTCATATCTAATGAATAACAATATATTAATTGATATAATAGTTGATTGAAGTAT
AACGAACAGGAGTCCATCAT

Product: radical activating enzyme family protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTIS
GGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV

Sequences:

>Translated_237_residues
MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTIS
GGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
>Mature_236_residues
AKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISG
GNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFD
DKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=138, Percent_Identity=32.6086956521739, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 26821; Mature: 26690

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAE
CCCCCEEEEECCCCCCCCEEEEEEEEEEEECCCCCEEECCCCCEECCCCCCCCEEEEEHH
EIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQI
HHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHH
DDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFDDKDYDFAKMIHHRYPDIPF
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCE
YLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
EEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCC
>Mature Secondary Structure 
AKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAE
CCCCEEEEECCCCCCCCEEEEEEEEEEEECCCCCEEECCCCCEECCCCCCCCEEEEEHH
EIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQI
HHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHH
DDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFDDKDYDFAKMIHHRYPDIPF
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCE
YLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
EEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]