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The map label for this gene is mleN [H]

Identifier: 57650868

GI number: 57650868

Start: 2379517

End: 2380830

Strand: Reverse

Name: mleN [H]

Synonym: SACOL2319

Alternate gene names: 57650868

Gene position: 2380830-2379517 (Counterclockwise)

Preceding gene: 57650869

Following gene: 57650867

Centisome position: 84.74

GC content: 35.46

Gene sequence:

>1314_bases
ATGGAAGTACATAAAAAGAGCAATGCTTGGGCATTATTCCCCTTGTTATTATTTGTGGCGTTGTTTTTAGGCGTAGGTAT
TATCACAGGTGATTTTACTTCAATGCCATTAAATGTTGCAATTACGATAACGGTAATTGTGGCGTTATTAATGAATCGAA
AAGAATCATTTGCTAAAAAAGTTGAGGTCTTCACGAAAGGTGCAGGCCATTCAAACATTGTTTTAATGATGTTGATTTTT
ATTTTAGCAGGTGCATTTTCAAATACAGCTGAAAAAATGGGTGGCGTAAAGTCGACAGTTAATTTAGGACTATCATTAAT
CCCAGAAAATTTAATTATTGTAGGATTGTTTGTGATTTGTATGTTTGTTTCTATATCTATGGGGACGTCTGTAGGAACTG
TTGCTGCAATAGCACCTGTTGGTTACGGATTTGCACAAGCGACAGATGTACCAACTGCGCTAGCAATGGCTACAGTTGTT
GGAGGTGCAATGTTCGGCGATAATCTATCAATGATTTCAGATACAACAATTGCTGCGGTTAGAACACAACATACGAAAAT
GAAAGATAAATTCAGAGTAAACTTTAAAATTGTATTGCCAGGTGCTATTTTAACGATTATTATTCTGTATTTCTTAACGA
ATGGCATTTCTTTAAATCATGCTAAAAACTATGATTATAATCTAATTAAAGTTGTACCGTATATCTTAGTATTGGTATTA
GCATTATTAGGTGTAAATGTCATTATCGTATTGATTGGCGGAACATTATTAGCCGGAATCATTGGTCTCATAGATGGTTC
ATTCGGTTGGATGGGGCTACTAGATGCAGTGTCCAAAGGTATTATAAGCATGGAAGATATTGCTATGATTGCGTTGCTAA
TTGGTGGTTTAGTCGGTATTATTCAACACAATGGTGGTATTGAATGGTTGTTGCAATTTGTTCGCTCTAAAGTGAAATCA
AAGCGTGGCGCAGAATTAGGAATTGCTAGTTTGGTAAGTGTCGCAGATATTGCCACGGCAAACAACACCATCTCTATTAT
TATGTCAGGTCCTTTAGCTAAAAATATTGCAGATGAATATGATGTAGATTCAAGGAAATCAGCAAGTATTCTTGATATAT
TTGGAGGCTGCTTCCAAGGTTTATTACCATATAGCCCGCAAGTCATTTCAGCGGCTGGTGTAGCTGGTATTTCACCGTTA
ATGTTATTCCCATACAGCATTTATCCTATATTATTAGGTGTATGCGGACTTATTGCGATTTTATTTAATTTTCCAAAGCT
AAAAAAGAACACTAGTCAAGAAATGAAACATTAA

Upstream 100 bases:

>100_bases
ACCGTAATGGTTTGAAGATAAGCAGGTAAAGCACATGAAAACCTCTTTCTTCATCGTTTGTGAGAAAGAGGTATTTTTAA
TTGGAAAGCAGGTAAAAAGG

Downstream 100 bases:

>100_bases
TGCTTAACAATATATCGATGTAGCCAAGTAAGGTGGCTTGTTATATTAACAGATCATGTTGTACTCACCGTATAACGCTA
ATCTTTTAAAAATGATGATC

Product: Na+/H+ antiporter family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIF
ILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVL
ALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKS
KRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPL
MLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

Sequences:

>Translated_437_residues
MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIF
ILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVL
ALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKS
KRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPL
MLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH
>Mature_437_residues
MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIF
ILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVL
ALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKS
KRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPL
MLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 46325; Mature: 46325

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVIC
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCHHHCCHHHHHHHHHHHH
MFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAV
HHHHHHCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHH
RTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVL
HHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEY
HHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHC
DVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAI
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFNFPKLKKNTSQEMKH
HHCCCHHCCCCHHHCCC
>Mature Secondary Structure
MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVIC
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCHHHCCHHHHHHHHHHHH
MFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAV
HHHHHHCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHH
RTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVL
HHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEY
HHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHC
DVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAI
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFNFPKLKKNTSQEMKH
HHCCCHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]