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The map label for this gene is lacE [H]

Identifier: 57650777

GI number: 57650777

Start: 2263934

End: 2265652

Strand: Reverse

Name: lacE [H]

Synonym: SACOL2181

Alternate gene names: 57650777

Gene position: 2265652-2263934 (Counterclockwise)

Preceding gene: 57650778

Following gene: 57650776

Centisome position: 80.64

GC content: 35.78

Gene sequence:

>1719_bases
ATGACAATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCACGTAACATATATTT
AAGAGCCATCCGTGATGGATTTATTTCTGCAATGCCTGTCATCTTATTCTCAAGTATCTTTCTATTAATTGCATATGTGC
CAAATATATTTGGTTTTAAATGGGATAAAGGCATGGAAGCCATTTTAATGAAACCATATAACTATACGATGGGGCTTGTA
GCATTTTTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTAGAAAGTACAAATCAAATTAA
CTTTATTTCTACAATGCTAGCAGCGATGTGTGGATTCTTGTTCTTAGCTTCTGATCCAGCCAAAGATGGTGGATTCTTAA
GTGCATTTATGGGAACTAAAGGTTTATTAACAGCATTCTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTG
AAGCGCAATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTTAAGGACTTAATTCCATTTTC
AGCGGTAATCATCATTCTTTATGCATTAGATTTAGTCATTCGCAACAGCTTTAAATCAAATGTAGCGGAAGGTATTTTAA
AATTATTCGAACCATTATTTACAGCAGCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGG
TTTGTAGGTATTCATGGTCCGTCAATTGTAGAGCCAGCAATTGCAGCCATTACATATGCGAATATCGAAGCGAACTTCAA
GTTGCTTCAAGCTGGAGAACACGCAGATAAAATTATTACATCAGGTACACAAATGTTTATCGTTACATTTGGAGGTACGG
GTGCAACATTGGTCGTCCCGTTCATGTTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTT
GTACCAACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAATCCTGTGTTCTTTATTCCATT
TGTATTAGCACCAATTGTTAACGTATGGATTTTCAAACTTTTCGTTGAAGTGTTAGGAATGAATAGTTTTAGTGTGAATT
TACCTTGGACAACACCAGGTCCATTGGGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACT
TTGATTGTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATTCTTGATGAAGAAGAAGGACG
TAAAGAAAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCAAACTTTGATACGAAAAAAGCTGATTCAATTTTAGCGGCAA
GTGGTGTATCAGACGATGCAGCTAAAGCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGT
ACAAGTGGATTATTAGCCAATGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTGAAAGCGGCAGCTGGCGGATA
TGGCGCACATATGGACATTATGAAAGAGTACCAATTAATCATTTTAGCGCCACAAGTTGCGTCTAACTATGAAGATATTA
AGCAAGATACAGATAGATTAGGTATTAAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAA
GCAGCCCTTGATTTCGTTCAACAACAATTTGAAAATTAA

Upstream 100 bases:

>100_bases
CATACAGTGTAACGATGATGCATGGTCAAGATCATTTAATGACAACAATTTTACTGAAAGATTTAATGAAGCATTTATTA
GAATTTTATAAAAGAGGGTG

Downstream 100 bases:

>100_bases
GAATAGGAGTTTTTCATATGACTAAAACATTACCTGAAGATTTTATTTTTGGTGGAGCAACAGCAGCATATCAAGCAGAA
GGTGCGACAAATACGGATGG

Product: PTS system, lactose-specific IIBC components

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MTMMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLV
AFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCV
KRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFW
FVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASV
VPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAIT
LIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGG
TSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQ
AALDFVQQQFEN

Sequences:

>Translated_572_residues
MTMMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLV
AFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCV
KRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFW
FVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASV
VPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAIT
LIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGG
TSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQ
AALDFVQQQFEN
>Mature_571_residues
TMMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVA
FLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVK
RNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWF
VGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVV
PTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITL
IVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGT
SGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQA
ALDFVQQQFEN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=477, Percent_Identity=26.8343815513627, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 62665; Mature: 62534

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEECCCCCCEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TMMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFK
CHHHHHHHHHHCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFL
CCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRNITIKMPKEVPPNISQVF
HHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHH
KDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVP
HHHCCCCHHHHHHHHHHEEECCCCCCCEEECCCHHHHHHHCCCEEEEEEECCCCHHHHHH
FMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKL
HHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHH
FVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLIVVDIIIYYPFLKVYDSEI
HHHHHCCCCEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGG
HCCHHCCCCCCHHHHHHHHCCCCCHHHCCHHHCCCCCCHHHHHCCCCCCCCEEEEECCCC
TSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRL
CHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHCCEEEEECCHHHCCHHHHHHHHHHH
GIKLAKTQGAEYIKLTRDGQAALDFVQQQFEN
CEEEECCCCCCEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA