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The map label for this gene is lacG [H]

Identifier: 57650776

GI number: 57650776

Start: 2262504

End: 2263916

Strand: Reverse

Name: lacG [H]

Synonym: SACOL2180

Alternate gene names: 57650776

Gene position: 2263916-2262504 (Counterclockwise)

Preceding gene: 57650777

Following gene: 57650775

Centisome position: 80.58

GC content: 34.82

Gene sequence:

>1413_bases
ATGACTAAAACATTACCTGAAGATTTTATTTTTGGTGGAGCAACAGCAGCATATCAAGCAGAAGGTGCGACAAATACGGA
TGGTAAAGGTCGCGTAGCATGGGATACGTATTTAGAAGAAAACTATTGGTACACTGCAGAACCAGCGAGTGATTTTTATG
ACAGATATCCAGTGGATTTAGAATTAAGTGAAAAATTCGGTGTTAATGGTATCCGAATTTCAATTGCATGGTCTCGTATC
TTCCCAAATGGATATGGCGAAGTTAACCCTAAAGGTGTTGAGTATTACCATAAGTTATTTGCAGAGTGTCATAAACGTCA
TGTTGAACCATTTGTAACATTGCATCATTTTGATACACCAGAAGTGTTACACAAAGATGGCGATTTTCTAAATCGTAAAA
CGATTGACTACTTTGTAGATTATGCTGAATATTGTTTTAAAGAATTCCCAGAAGTAAAATATTGGACTACATTCAATGAA
ATTGGACCTATTGGTGATGGTCAATATTTAGTAGGTAAATTCCCTCCAGGTATTAAATATGATTTTGAAAAAGTATTCCA
ATCACATCATAACATGATGGTGGCTCATGCTAGAGCAGTAAAATTATTTAAAGATGGCGGATACAAAGGAGAAATTGGTG
TTGTACACGCATTGCCAACAAAATATCCGTTCGATCCATCAAATCCTGAAGATGTTCGTGCAGCAGAATTAGAAGATATC
ATCCATAATAAATTCATCTTAGATGCAACATATTTAGGTAAGTATTCTCGTGAAACGATGGAAGGTGTGCAACATATCTT
ATCTGTTAATGGCGGTAAATTAAACATTACAGATGAAGATTATGCCATTTTAGATGCAGCCAAAGATTTAAACGACTTCT
TAGGTATCAATTACTACATGAGTGATTGGATGAGAGGTTACGATGGAGAATCTGAAATCACGCATAATGCGACAGGTGAT
AAAGGCGGTTCTAAATACCAACTTAAAGGTGTCGGACAACGTGAATTTGATGTCGACGTACCTCGCACAGACTGGGACTG
GATGATCTATCCTCAAGGTTTATATGATCAAATCATGCGTGTTGTTAAAGATTATCCTAACTATCATAAGATTTATATCA
CTGAAAATGGTTTAGGATATAAAGATGAATTTATTGAATCTGAAAAAACAGTTCATGATGATGCACGTATTGATTATGTA
AGACAACATTTGAACGTTATTGCAGATGCTATTATAGATGGTGCAAATGTTAAAGGTTACTTTATTTGGTCATTAATGGA
TGTGTTCTCTTGGTCAAATGGTTATGAAAAACGATATGGTTTATTCTATGTCGATTTTGAGACTCAAGAACGTTATCCTA
AGAAAAGTGCTTATTGGTACAAAGAGTTAGCCGAAACAAAAGAAATTAAATAA

Upstream 100 bases:

>100_bases
AGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAAGCAGCCCTTGATTTCGTTCAACAACAATTTGAAAAT
TAAGAATAGGAGTTTTTCAT

Downstream 100 bases:

>100_bases
TTATAGATATTAAAAGGCCTATCAGTCGAGTGCTGGTAGGTCTTTATTTATAGAAAGAGATAAATTAAACATGCATATAC
ATATTTATATATTAATCAAT

Product: 6-phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase; PGALase; P-beta-Gal; PBG [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYDRYPVDLELSEKFGVNGIRISIAWSRI
FPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGD
KGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYV
RQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK

Sequences:

>Translated_470_residues
MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYDRYPVDLELSEKFGVNGIRISIAWSRI
FPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGD
KGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYV
RQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK
>Mature_469_residues
TKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYDRYPVDLELSEKFGVNGIRISIAWSRIF
PNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEI
GPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHALPTKYPFDPSNPEDVRAAELEDII
HNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDK
GGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYVR
QHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK

Specific function: Can Hydrolyze Salicin, Cellobiose, And Probably Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=479, Percent_Identity=31.5240083507307, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI110681710, Length=480, Percent_Identity=29.7916666666667, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI32481206, Length=484, Percent_Identity=29.9586776859504, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI24497614, Length=492, Percent_Identity=28.2520325203252, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI28376633, Length=201, Percent_Identity=36.81592039801, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI1789070, Length=494, Percent_Identity=34.6153846153846, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI2367174, Length=493, Percent_Identity=32.0486815415822, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI2367270, Length=498, Percent_Identity=32.7309236947791, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17552856, Length=495, Percent_Identity=31.5151515151515, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17539390, Length=493, Percent_Identity=29.6146044624746, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21356577, Length=487, Percent_Identity=28.7474332648871, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.85 [H]

Molecular weight: Translated: 54551; Mature: 54420

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYDRYPVDL
CCCCCCCHHHCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHCCCCCE
ELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTP
EEHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEIGPIGDGQYLVGKFPPGIKY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEECCCCCCCH
DFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
HHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYM
HHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHH
SDWMRGYDGESEITHNATGDKGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMR
HHHHHCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHH
VVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYVRQHLNVIADAIIDGANVKGY
HHHCCCCCEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
FIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK
HHHHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYDRYPVDL
CCCCCCHHHCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHCCCCCE
ELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTP
EEHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEIGPIGDGQYLVGKFPPGIKY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEECCCCCCCH
DFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
HHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYM
HHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHH
SDWMRGYDGESEITHNATGDKGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMR
HHHHHCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHH
VVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYVRQHLNVIADAIIDGANVKGY
HHHCCCCCEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
FIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK
HHHHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA