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The map label for this gene is cls2

Identifier: 57650761

GI number: 57650761

Start: 2144652

End: 2146136

Strand: Direct

Name: cls2

Synonym: SACOL2079

Alternate gene names: 57650761

Gene position: 2144652-2146136 (Clockwise)

Preceding gene: 57650757

Following gene: 57650762

Centisome position: 76.34

GC content: 32.66

Gene sequence:

>1485_bases
ATGATAGAGTTATTATCCATTGCACTCAAGCATTCTAATATTATTTTAAATTCAATATTTATTGGTGCATTTATTTTAAA
CTTATTATTCGCCTTTACCATTATTTTCATGGAAAGACGTTCTGCCAATTCTATCTGGGCTTGGTTACTAGTCTTAGTTT
TCTTGCCTTTATTCGGCTTCATTTTATACTTACTATTAGGACGACAAATTCAACGTGACCAAATTTTCAAAATTGATAAG
GAAGATAAAAAAGGATTAGAGTTAATCGTTGATGAGCAATTAGCTGCTTTAAAAAATGAAAACTTTTCAAATTCCAATTA
TCAAATTGTAAAATTTAAAGAAATGATTCAAATGTTGTTATATAATAACGCAGCATTTTTAACAACAGACAACGATTTAA
AAATATACACAGACGGCCAAGAAAAATTTGATGACCTAATACAAGACATCCGTAATGCTACTGATTATATTCATTTTCAG
TACTATATTATTCAAAATGATGAATTAGGTCGTACCATTTTAAATGAACTTGGTAAAAAAGCGGAACAAGGTGTAGAAGT
TAAAATTCTTTATGATGACATGGGTTCTCGTGGACTGCGTAAAAAAGGCTTACGCCCGTTTCGCAATAAAGGTGGACATG
CTGAAGCATTTTTCCCATCAAAATTACCTTTAATTAACTTGCGTATGAACAATCGAAACCATCGAAAAATTGTTGTAATA
GATGGGCAAATTGGATATGTTGGTGGTTTTAATGTTGGTGATGAGTACTTAGGTAAATCAAAAAAATTCGGCTATTGGCG
AGATACGCATTTACGAATTGTCGGGGATGCAGTGAATGCATTGCAATTACGATTTATTCTAGATTGGAATTCACAAGCCA
CACGTGACCACATCTCCTATGATGATCGTTATTTCCCAGATGTAAATTCTGGTGGAACAATTGGCGTTCAAATAGCTTCT
AGTGGTCCTGACGAAGAATGGGAACAGATTAAATACGGCTATTTGAAAATGATTTCATCTGCTAAAAAATCGATTTATAT
TCAATCTCCCTATTTCATACCTGATCAAGCCTTTTTAGATTCTATTAAAATTGCGGCATTAGGTGGTGTTGATGTCAATA
TCATGATTCCTAATAAACCTGACCATCCGTTTGTTTTTTGGGCTACTTTAAAAAATGCAGCATCCTTATTAGATGCCGGT
GTTAAAGTATTTCACTACGACAATGGCTTTTTACACTCAAAAACACTTGTTATAGATGATGAAATTGCAAGTGTGGGAAC
AGCTAATATGGACCATCGCAGTTTCACATTGAATTTCGAAGTCAACGCTTTTATTTATGACCAACAAATTGCCAAAAAAT
TAAAACAAGCTTTTATAGATGATTTAGCAGTATCTTCTGAATTAACAAAAGCACGTTATGCTAAGCGAAGTCTTTGGATT
AAATTTAAAGAAGGTATTTCACAATTATTGTCACCTATCTTATAA

Upstream 100 bases:

>100_bases
AGTGTGTTTTTCAAACTAAATTGTCATGATATTTAAATTGCTTCATGATACAATTAATAAGATATACTACTCGTTTAAGA
TTTTCGATGGGAGGTTTCAA

Downstream 100 bases:

>100_bases
AATAGAAATATGAGGAGTGTAACTTTAATGCAACAATCAGACGTCATTAGTGCTGCCAAAAAATATATGGAATCTATTCA
TCAAAATGATTATACAGGCC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDK
EDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
DGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIAS
SGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAG
VKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
KFKEGISQLLSPIL

Sequences:

>Translated_494_residues
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDK
EDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
DGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIAS
SGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAG
VKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
KFKEGISQLLSPIL
>Mature_494_residues
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDK
EDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
DGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIAS
SGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAG
VKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
KFKEGISQLLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=478, Percent_Identity=30.5439330543933, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787007, Length=362, Percent_Identity=27.9005524861878, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS2_STAAC (Q5HEB2)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186895.1
- ProteinModelPortal:   Q5HEB2
- STRING:   Q5HEB2
- EnsemblBacteria:   EBSTAT00000010211
- GeneID:   3238401
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL2079
- TIGR:   SACOL2079
- eggNOG:   COG1502
- GeneTree:   EBGT00050000023642
- HOGENOM:   HBG694312
- OMA:   HRFILDW
- ProtClustDB:   CLSK885759
- BioCyc:   SAUR93062:SACOL2079-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56519; Mature: 56519

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50035 PLD; PS00136 SUBTILASE_ASP

Important sites: ACT_SITE 234-234 ACT_SITE 236-236 ACT_SITE 241-241 ACT_SITE 412-412 ACT_SITE 414-414 ACT_SITE 419-419

Signals:

None

Transmembrane regions:

HASH(0xfbd7a80)-; HASH(0xcc69110)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGF
CHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLL
HHHHHHCCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
YNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKK
HCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHH
AEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
HHCCCEEEEEEECCCCCCHHHHCCCCHHCCCCCEEEECCCCCCEEEEEECCCCCCEEEEE
DGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISY
ECCEEEECCCCCCHHHHCCCCCCCCEECCEEEEEECCCCEEEEEEEEECCCCCCCCCCCC
DDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHC
SIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDD
CEEEEEECCEEEEEEECCCCCCCEEEEEEHHHHHHHHHCCEEEEEECCCEEECEEEEEEC
EIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
HHHHCCCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
KFKEGISQLLSPIL
EHHHHHHHHHHHCH
>Mature Secondary Structure
MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGF
CHHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLL
HHHHHHCCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
YNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKK
HCCEEEEEECCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHH
AEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVI
HHCCCEEEEEEECCCCCCHHHHCCCCHHCCCCCEEEECCCCCCEEEEEECCCCCCEEEEE
DGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISY
ECCEEEECCCCCCHHHHCCCCCCCCEECCEEEEEECCCCEEEEEEEEECCCCCCCCCCCC
DDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLD
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHC
SIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDD
CEEEEEECCEEEEEEECCCCCCCEEEEEEHHHHHHHHHCCEEEEEECCCEEECEEEEEEC
EIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWI
HHHHCCCCCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
KFKEGISQLLSPIL
EHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA