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The map label for this gene is pcrA

Identifier: 57650656

GI number: 57650656

Start: 2028699

End: 2030891

Strand: Reverse

Name: pcrA

Synonym: SACOL1966

Alternate gene names: 57650656

Gene position: 2030891-2028699 (Counterclockwise)

Preceding gene: 57650657

Following gene: 57650655

Centisome position: 72.29

GC content: 34.15

Gene sequence:

>2193_bases
ATGAATGCGTTATTAAATCATATGAATACAGAGCAAAGTGAAGCTGTAAAGACAACAGAAGGACCATTGTTAATTATGGC
AGGTGCTGGTTCAGGGAAGACACGTGTTTTAACACATAGAATTGCTTATTTATTAGACGAAAAAGATGTCTCACCATACA
ATGTTTTGGCTATTACTTTTACAAATAAAGCTGCAAGAGAAATGAAAGAACGTGTTCAAAAATTAGTAGGTGATCAAGCA
GAAGTTATTTGGATGTCAACATTCCACTCAATGTGTGTTCGTATTTTACGTCGTGATGCAGATCGAATTGGTATAGAACG
CAATTTTACGATAATTGATCCTACAGACCAAAAATCTGTTATTAAAGACGTCTTAAAAAATGAAAATATTGATAGTAAAA
AGTTTGAACCTCGTATGTTTATCGGTGCGATCAGTAATTTGAAAAATGAACTTAAAACACCTGCAGATGCTCAAAAAGAA
GCCACAGATTATCACTCGCAAATGGTAGCAACGGTTTATAGTGGATATCAACGCCAATTGTCACGTAATGAAGCGTTAGA
TTTTGATGACCTTATTATGACAACGATTAACTTATTTGAGCGTGTACCAGAAGTTCTAGAATATTATCAGAACAAATTCC
AATATATTCATGTAGATGAGTATCAAGATACTAATAAAGCACAATACACATTAGTTAAATTATTAGCAAGTAAGTTTAAA
AACTTATGTGTTGTAGGTGACTCAGATCAGTCAATTTATGGTTGGCGTGGTGCTGATATTCAAAATATCTTATCATTTGA
AAAAGACTATCCAGAAGCGAATACAATCTTTTTAGAGCAAAATTATCGTTCGACGAAAACGATTTTAAATGCGGCTAACG
AAGTGATTAAAAATAATTCTGAACGTAAGCCAAAAGGACTGTGGACTGCAAATACGAATGGTGAGAAAATTCATTACTAT
GAAGCAATGACGGAACGTGATGAAGCGGAATTTGTAATACGAGAAATTATGAAGCATCAACGTAATGGTAAGAAATATCA
AGATATGGCAATTTTATATAGAACGAATGCACAATCACGTGTACTTGAGGAAACATTCATGAAATCTAATATGCCATACA
CAATGGTTGGTGGCCAAAAGTTCTATGACCGTAAAGAAATCAAAGATTTATTAAGTTATTTGCGTATAATTGCCAATAGT
AATGATGACATTAGTTTGCAACGTATTATTAATGTTCCAAAAAGAGGTGTAGGTCCTTCATCTGTTGAAAAAGTTCAAAA
CTATGCACTTCAAAACAATATCAGTATGTTTGATGCACTTGGAGAAGCTGATTTTATCGGATTGTCAAAAAAGGTGACAC
AAGAGTGTCTTAATTTTTACGAGTTAATCCAAAGCTTGATAAAAGAACAAGAATTTTTAGAAATTCATGAAATAGTTGAT
GAAGTATTACAAAAGTCTGGCTATCGAGAAATGCTTGAACGTGAAAATACATTAGAATCTCGAAGTAGATTAGAAAACAT
CGATGAATTTATGTCAGTACCAAAAGACTATGAGGAAAATACCCCATTAGAAGAACAGTCATTAATTAACTTTTTAACGG
ATTTATCGTTAGTAGCTGATATTGATGAGGCAGATACTGAAAATGGCGTAACACTAATGACGATGCACTCGGCTAAGGGC
CTTGAATTTCCAATTGTCTTTATAATGGGGATGGAAGAATCTTTATTCCCACATATTAGAGCGATTAAGAGTGAAGATGA
TCATGAAATGCAAGAAGAACGTCGTATTTGTTATGTAGCAATTACAAGGGCTGAAGAGGTGTTATATATCACTCATGCGA
CATCAAGAATGTTATTTGGTCGCCCTCAGTCAAATATGCCATCCAGATTTTTAAAGGAAATTCCAGAATCACTATTAGAA
AATCATTCAAGTGGCAAACGACAAACGATACAACCTAAGGCAAAACCTTTTGCTAAACGCGGATTTAGTCAACGAACAAC
GTCAACGAAAAAACAAGTATTGTCATCTGATTGGAATGTAGGTGACAAAGTGATGCATAAAGCCTGGGGAGAAGGCATGG
TGAGTAATGTAAACGAGAAAAATGGCTCAATCGAACTAGATATTATCTTTAAATCACAAGGGCCAAAACGTTTGTTAGCG
CAATTTGCACCAATTGAAAAAAAGGAGGATTAA

Upstream 100 bases:

>100_bases
CAGAGATGGCAGCTATTGCAGATACAATTATCGTCGGTGATATTATTTATAAAGATATTAAAAAAGCTTTAAAAACAGTA
AAAATAAAGGAGTCTAGTAA

Downstream 100 bases:

>100_bases
GGGATGGCTGATTTATCGTCTCGTGTGAACGAGTTACATGATTTATTAAATCAATACAGTTATGAATACTATGTAGAGGA
TAATCCATCTGTACCAGATA

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQA
EVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFK
NLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYY
EAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANS
NDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVD
EVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKG
LEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLE
NHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLA
QFAPIEKKED

Sequences:

>Translated_730_residues
MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQA
EVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFK
NLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYY
EAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANS
NDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVD
EVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKG
LEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLE
NHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLA
QFAPIEKKED
>Mature_730_residues
MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQA
EVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFK
NLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYY
EAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANS
NDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVD
EVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKG
LEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLE
NHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLA
QFAPIEKKED

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367296, Length=726, Percent_Identity=39.8071625344353, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI48994965, Length=679, Percent_Identity=38.880706921944, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1787196, Length=362, Percent_Identity=27.0718232044199, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=752, Percent_Identity=29.1223404255319, Blast_Score=260, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6324477, Length=660, Percent_Identity=23.3333333333333, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): PCRA_STAA8 (Q53727)

Other databases:

- EMBL:   M63176
- EMBL:   CP000253
- PIR:   S39923
- RefSeq:   YP_500614.1
- ProteinModelPortal:   Q53727
- SMR:   Q53727
- STRING:   Q53727
- EnsemblBacteria:   EBSTAT00000028219
- GeneID:   3921194
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_02123
- eggNOG:   COG0210
- GeneTree:   EBGT00050000024857
- HOGENOM:   HBG467281
- OMA:   GIKWLIA
- ProtClustDB:   CLSK885640
- BioCyc:   SAUR93061:SAOUHSC_02123-MONOMER
- GO:   GO:0005737
- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016
- Gene3D:   G3DSA:1.10.10.160
- PANTHER:   PTHR11070
- TIGRFAMs:   TIGR01073

Pfam domain/function: PF00580 UvrD-helicase

EC number: =3.6.4.12

Molecular weight: Translated: 84075; Mature: 84075

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS51198 UVRD_HELICASE_ATP_BIND; PS51217 UVRD_HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITF
CCHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
TNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSV
CCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHH
IKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKEATDYHSQMVATVYSGYQRQL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
NLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNS
CEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
ERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSR
CCCCCCCEEECCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCHHH
VLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANSNDDISLQRIINVPKRGVGPS
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHH
SVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVD
HHHHHHHHHHHCCCHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVAD
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
IDEADTENGVTLMTMHSAKGLEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVA
CCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHCCEEEEE
ITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKR
EECCCEEEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHC
GFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLA
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCCCHHHHHH
QFAPIEKKED
HHCCCCCCCC
>Mature Secondary Structure
MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITF
CCHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
TNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSV
CCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHH
IKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKEATDYHSQMVATVYSGYQRQL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
NLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNS
CEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
ERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSR
CCCCCCCEEECCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCHHH
VLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANSNDDISLQRIINVPKRGVGPS
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHH
SVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVD
HHHHHHHHHHHCCCHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVAD
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
IDEADTENGVTLMTMHSAKGLEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVA
CCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHCCEEEEE
ITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKR
EECCCEEEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHC
GFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLA
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCCCHHHHHH
QFAPIEKKED
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203