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The map label for this gene is pfkA

Identifier: 57650569

GI number: 57650569

Start: 1783705

End: 1784673

Strand: Reverse

Name: pfkA

Synonym: SACOL1746

Alternate gene names: 57650569

Gene position: 1784673-1783705 (Counterclockwise)

Preceding gene: 57650570

Following gene: 57650568

Centisome position: 63.52

GC content: 37.05

Gene sequence:

>969_bases
ATGAAGAAAATTGCAGTTTTAACTAGTGGTGGAGATTCACCTGGAATGAATGCTGCCGTAAGAGCAGTTGTTCGTACAGC
AATTTACAATGAAATTGAAGTTTATGGTGTGTATCATGGTTACCAAGGATTGTTAAATGATGATATTCATAAACTTGAAT
TAGGATCAGTTGGGGATACGATTCAGCGTGGAGGTACATTCTTGTATTCAGCAAGATGTCCAGAGTTTAAGGAGCAAGAA
GTACGTAAAGTTGCAATCGAAAACTTACGTAAAAGAGGGATTGAGGGCCTTGTAGTTATTGGTGGTGACGGTAGTTATCG
CGGTGCACAACGCATCAGTGAGGAATGTAAAGAAATTCAAACTATCGGTATTCCTGGTACGATTGACAATGATATCAATG
GTACTGATTTTACAATTGGATTTGACACAGCATTAAATACGATTATTGGCTTAGTCGACAAAATTAGAGATACTGCGTCA
AGTCACGCACGAACATTTATCATTGAAGCAATGGGCCGTGATTGTGGAGATCTAGCATTATGGGCTGGATTATCAGTTGG
TGCTGAGACAATTGTAGTTCCAGAAGTGAAAACAGATATTAAAGAAATAGCTGATAAAATTGAACAAGGTATTAAACGTG
GTAAGAAACACTCAATCGTTCTTGTAGCAGAAGGTTGTATGACTGCGCAAGATTGTCAAAAAGAATTATCACAATACATC
AATGTTGATAATAGAGTGTCTGTGTTAGGTCACGTTCAACGTGGTGGTAGCCCAACAGGTGCGGATAGAGTTTTAGCATC
ACGTTTAGGTGGATATGCGGTAGACTTATTAATGCAAGGTGAAACAGCTAAGGGTGTTGGAATTAAGAACAATAAAATTG
TAGCAACATCTTTTGATGAAATTTTTGATGGTAAAGATCATAAATTTGATTATAGTCTATATGAACTTGCTAACAAGTTA
TCTATATAA

Upstream 100 bases:

>100_bases
ACGTTTACATCAATTCAATACATTAGTTTTGATGGAATGACATATCAATTTGTGGTAATTTAGAGTTAAAGATAAATCAG
TTATAGAAAGGTATGTCGTC

Downstream 100 bases:

>100_bases
GATTTCAGGAGGAATTATAAAATGAGAAAAACTAAAATTGTATGTACAATTGGACCAGCTTCAGAATCAGAAGAAATGAT
TGAGAAATTAATCAATGCTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQE
VRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYI
NVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKL
SI

Sequences:

>Translated_322_residues
MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQE
VRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYI
NVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKL
SI
>Mature_322_residues
MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQE
VRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYI
NVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKL
SI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=43.9102564102564, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=43.9102564102564, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=43.9102564102564, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=43.9102564102564, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=42.9487179487179, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=40.8360128617363, Blast_Score=215, Evalue=5e-56,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=50.3125, Blast_Score=318, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=42.1221864951768, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=324, Percent_Identity=35.4938271604938, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI72003601, Length=348, Percent_Identity=31.3218390804598, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321679, Length=330, Percent_Identity=41.2121212121212, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323861, Length=331, Percent_Identity=38.0664652567976, Blast_Score=205, Evalue=9e-54,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=44.5512820512821, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24652337, Length=312, Percent_Identity=44.5512820512821, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=44.5512820512821, Blast_Score=237, Evalue=7e-63,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_STAA2 (A6U2G5)

Other databases:

- EMBL:   CP000736
- RefSeq:   YP_001316920.1
- ProteinModelPortal:   A6U2G5
- SMR:   A6U2G5
- STRING:   A6U2G5
- EnsemblBacteria:   EBSTAT00000015063
- GeneID:   5315767
- GenomeReviews:   CP000736_GR
- KEGG:   sah:SaurJH1_1789
- eggNOG:   COG0205
- GeneTree:   EBGT00050000024765
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- ProtClustDB:   PRK03202
- BioCyc:   SAUR359787:SAURJH1_1789-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34840; Mature: 34840

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 129-129 BINDING 164-164 BINDING 245-245 BINDING 251-251 BINDING 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDT
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEECCCCCHH
IQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQ
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLAL
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCHHHH
WAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYI
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHC
NVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDE
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEHHH
IFDGKDHKFDYSLYELANKLSI
HHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDT
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEECCCCCHH
IQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQ
HHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLAL
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCHHHH
WAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYI
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHC
NVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDE
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEHHH
IFDGKDHKFDYSLYELANKLSI
HHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA