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The map label for this gene is queA [H]

Identifier: 57650521

GI number: 57650521

Start: 1727737

End: 1728762

Strand: Reverse

Name: queA [H]

Synonym: SACOL1695

Alternate gene names: 57650521

Gene position: 1728762-1727737 (Counterclockwise)

Preceding gene: 57650522

Following gene: 57650520

Centisome position: 61.53

GC content: 33.33

Gene sequence:

>1026_bases
GTGAATATTGAAGAATTTGACTATGACTTACCAGAATCATTAATTGCTCAAACGCCTTTAAAAGATCGTGATCATAGTCG
TTTATTAGTCATGGATAGAGAAACTGGTGAAATGAAACATTTACATTTCAAAGATATCATTGAGTATTTTAGACCTGGTG
ATACATTAGTGCTTAACGATACGCGAGTAATGCCAGCTAGACTTTTTGGTTTAAAAGAAGAAACTGGTGCAAAAGTTGAA
ATGTTAATGTTAACTCAAATTGAAGGTAATGATTGGGAAGTCTTACTGAAACCAGCTAAGCGTATTAAAGTTGGTAATAA
ATTGAATTTTGGTAATGGCAAAATTATAGCTGAATGCATAAAAGAAATGGATCAAGGTGGACGCATCATGCGTTTACATT
ATGAAGGTATTTTACAAGAAAGATTAGATGAATTAGGGGAAATGCCACTGCCACCATACATCAAAGAACGTTTAGATGAT
CCAGATCGTTATCAAACAGTTTACGCTAAAGAAAGTGGTTCAGCGGCAGCACCAACAGCAGGATTACATTTTACTGATGA
GTTATTAATTGAAATTAAAAATAAAGGTGTTAATATCGCATTTGTTACATTACATGTTGGGTTAGGTACGTTTAGACCGG
TGAGCGTAGACGATGTGAATGACCACGAAATGCATAGTGAATATTATCAAATGACACAAGAAACAGCTGATTTATTAAAT
GATACTAAGTCCAAAGGACATCGCATTATATCAGTTGGTACAACTTCAACACGTACACTTGAAACAATTCGACGCGATCA
TGATAAATTTGTTGAAACGAGTGGCTGGACTAATATATTTATTTATCCAGGATTTGATTTTAAAGCAATTGATGGCCAGA
TTACTAATTTTCATTTACCAAAATCAACATTAGTTATGCTAGTATCAGCGTTTAGTAGTCGTGAAAATGTTCTGAATGCT
TATAAAACGGCAGTAAATTTAGAATATAGATTCTTTAGTTTTGGCGATGCAATGTTAATTATATAA

Upstream 100 bases:

>100_bases
CTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCATTAGCTTATGAACATTTTGCAAAGTC
GAATGAGGAGAGAGAATAAC

Downstream 100 bases:

>100_bases
AAAGAATGTGAGGATTTTGAATATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAG
GTATCGTGCACACACCACAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVE
MLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNA
YKTAVNLEYRFFSFGDAMLII

Sequences:

>Translated_341_residues
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVE
MLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNA
YKTAVNLEYRFFSFGDAMLII
>Mature_341_residues
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVE
MLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNA
YKTAVNLEYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=45.3488372093023, Blast_Score=303, Evalue=8e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38969; Mature: 38969

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEC
TRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECI
CCCCHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHEECCCEEECCCCHHHHHHH
KEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTVYAKESGSAAAPTA
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHEEEEEECCCCCCCCCC
GLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
CCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLP
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEECC
KSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSFGDAMLII
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEC
>Mature Secondary Structure
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEC
TRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECI
CCCCHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHEECCCEEECCCCHHHHHHH
KEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTVYAKESGSAAAPTA
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHEEEEEECCCCCCCCCC
GLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
CCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLP
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEECC
KSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSFGDAMLII
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA