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The map label for this gene is tgt [H]

Identifier: 57650520

GI number: 57650520

Start: 1726575

End: 1727714

Strand: Reverse

Name: tgt [H]

Synonym: SACOL1694

Alternate gene names: 57650520

Gene position: 1727714-1726575 (Counterclockwise)

Preceding gene: 57650521

Following gene: 57650519

Centisome position: 61.5

GC content: 35.7

Gene sequence:

>1140_bases
ATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAGGTATCGTGCACACACCACACGG
TTCATTTGAAACACCTATGTTTATGCCAGTTGGTACTAAAGCAACCGTTAAAACAATGAGTCCAGAAGAGTTAAGACAAA
TTGAAGCAAAAATCATTTTGGGCAACACATATCATTTGTGGTTACAACCCGGAAATGATATTATCAAACACGCTGGGGGA
TTACATAAATTCATGAATTGGGATGGTCCGATTCTTACAGATTCAGGCGGTTTCCAAGTGTTTAGTTTAAGTAATTTACG
TAAAATTACAGAAGAAGGCGTGGAATTTAGACATCATACTAATGGGTCTAAATTATTTTTGAGTCCTGAGAAATCAATGC
AAATTCAAAATGATTTAGGATCTGATATTATGATGGCATTTGATGAATGTCCACCGATGCCTGCTGAATATGATTATGTA
AAAAAATCTATTGAACGTACAACACGTTGGGCGAAAAGATGTCTAGATGCACACCAAAGACCTGAAGATCAAGCATTGTT
CGGCATTATACAAGGTGGCGAATATGAAGATTTAAGAGAACAAAGTGCAAAGGATTTAGTAGAATTAGATTTTCCTGGTT
ATGCAATCGGCGGTTTGTCAGTTGGTGAACCTAAACCGGTTATGTATAAAATGGTTGAACATACAGAGCAGTTTATGCCT
AAAGATAAACCAAGATATTTAATGGGTGTAGGATCTCCAGATGCGTTAATCGAATGTAGTATTCGCGGCATGGATATGTT
TGATTGTGTCTTACCGACACGTATTGCCAGAAATGGTACTTGTATGACATCGCAAGGTCGTTTAGTTATTAAAAATGCAA
AATTTGCAGATGATTTAAGACCGTTAGATGAGAATTGTGACTGTTATACATGTCAAAACTATTCAAGAGCGTATATACGT
CATTTAATCAAGGCAGAGGAAACTTTTGGTATTCGTCTTACTACTATTCATAATTTACATTTTCTGCTAAAATTAATGGA
AGATATAAGACAAGCCATTCGAGAAGATCGTCTTTTAGATTTCAAAGAAGAATTCTTCGAGCAATATGGATTAAATGTTG
AGAACCCAAAAAACTTTTAA

Upstream 100 bases:

>100_bases
GTTCTGAATGCTTATAAAACGGCAGTAAATTTAGAATATAGATTCTTTAGTTTTGGCGATGCAATGTTAATTATATAAAA
AGAATGTGAGGATTTTGAAT

Downstream 100 bases:

>100_bases
GCAAGAGGAGCGTATAAAATGCAATTTTCATTACTAATATATATAGTCGTAATTTTTGCGGTTATGTATTTCTTGATGAT
CAGACCACAACAAAAACGTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGG
LHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIR
HLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF

Sequences:

>Translated_379_residues
MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGG
LHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIR
HLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF
>Mature_378_residues
PAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGL
HKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVK
KSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMPK
DKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRH
LIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=47.1074380165289, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI13375872, Length=291, Percent_Identity=24.3986254295533, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1786607, Length=358, Percent_Identity=56.9832402234637, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=47.3829201101928, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146502, Length=249, Percent_Identity=25.7028112449799, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=44.9864498644986, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI21355311, Length=270, Percent_Identity=27.4074074074074, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43310; Mature: 43179

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIIL
CCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEECCCHHHHHHHHEEEEE
GNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHT
CCEEEEEEECCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHEEECC
NGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQR
CCCEEEECCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
CCCCEEEEEECCCCHHHHHHHHHHHEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCC
KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLR
CCCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHCC
PLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLD
CCCCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCH
FKEEFFEQYGLNVENPKNF
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVKTMSPEELRQIEAKIIL
CCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEECCCHHHHHHHHEEEEE
GNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFSLSNLRKITEEGVEFRHHT
CCEEEEEEECCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHEEECC
NGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYVKKSIERTTRWAKRCLDAHQR
CCCEEEECCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGGLSVGEPKPVMYKMVEHTEQFMP
CCCCEEEEEECCCCHHHHHHHHHHHEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCC
KDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIARNGTCMTSQGRLVIKNAKFADDLR
CCCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHCC
PLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLTTIHNLHFLLKLMEDIRQAIREDRLLD
CCCCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCH
FKEEFFEQYGLNVENPKNF
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA