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The map label for this gene is bfmBAB [H]

Identifier: 57650473

GI number: 57650473

Start: 1596543

End: 1597526

Strand: Reverse

Name: bfmBAB [H]

Synonym: SACOL1561

Alternate gene names: 57650473

Gene position: 1597526-1596543 (Counterclockwise)

Preceding gene: 57650474

Following gene: 57650472

Centisome position: 56.86

GC content: 35.47

Gene sequence:

>984_bases
ATGGCTAAATTATCTTATTTAGAGGCGATACGCCAGGCACAAGACTTAGCATTACAACAAAATAAAGATGTGTTTATTCT
TGGTGAAGATGTTGGTAGAAAAGGTGGCGTATTTGGTACTACTCAAGGACTGCAACAAAAATATGGTGAAGACAGAGTTA
TCGATACGCCATTAGCTGAGTCAAATATTGTTGGAACTGCCATTGGTGCAGCTATGGTAGGTAAAAGACCAATTGCAGAA
ATTCAATTTGCAGATTTTATTTTACCTGCGACAAATCAAATTATTAGTGAAGCTGCGAAAATGCGTTATCGTTCAAATAA
TGATTGGCAATGCCCATTAACGATTCGTGCACCATTTGGTGGCGGTGTTCACGGAGGACTATATCATTCACAAAGTATTG
AAAGTATATTTGCTTCATCGCCAGGTTTAACAATTGTTATTCCTTCCACACCGTATGATGCTAAAGGGCTTTTATTATCA
TCTATCGAATCAAATGATCCAGTATTGTACTTTGAGCATAAAAAAGCTTATCGTTTCTTAAAGGAAGAAGTGCCAGAAGA
ATATTACACTGTACCTTTAGGAAAAGCAGATGTGAAACGTGAAGGTGAAGATTTAACAGTATTTTGTTATGGATTAATGG
TCAATTATTGTTTACAAGCAGCGGATATTTTAGCTGCAGACGGTATCAATGTTGAAGTCGTTGATTTACGAACTGTTTAT
CCATTAGATAAAGAAACAATTATTGATCGTGCTAAACATACAGGTAAAGTTTTACTTGTAACTGAAGATAATTTAGAAGG
TAGTATTATGTCAGAAGTGTCAGCGATTATTGCAGAGCATTGCTTGTTCGATTTAGATGCACCAATCATGCGTTTAGCTG
CTCCAGATGTACCATCTATGCCATTTTCTCCTGTATTAGAAAATGAAATTATGATGAATCCAGAAAAAATCTTAAATAAA
ATGCGTGAATTAGCAGAATTCTAG

Upstream 100 bases:

>100_bases
AAGACATCATCAATAAAGCGACAAAGGCTGCTGAAGACGCGCCATATCCAAGTGTCGAAGAAGCCTATGCATTTGTGTAC
GAAGAAGGGAGTCTTAACTA

Downstream 100 bases:

>100_bases
GGAGGGAAAGTCATGGAAATAACAATGCCTAAGTTAGGTGAGAGTGTTCATGAAGGCACCATTGAACAATGGTTAGTTTC
TGTTGGTGATCATATTGATG

Product: 2-oxoisovalerate dehydrogenase, E1 component subunit beta

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGLQQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAE
IQFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVY
PLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNK
MRELAEF

Sequences:

>Translated_327_residues
MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGLQQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAE
IQFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVY
PLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNK
MRELAEF
>Mature_326_residues
AKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGLQQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEI
QFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLSS
IESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVYP
LDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKM
RELAEF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=43.7308868501529, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=43.7308868501529, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI156564403, Length=310, Percent_Identity=36.4516129032258, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI291084858, Length=310, Percent_Identity=35.4838709677419, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI205277463, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4507521, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI133778974, Length=303, Percent_Identity=27.3927392739274, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI225637463, Length=306, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI225637459, Length=306, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI225637461, Length=306, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1786622, Length=248, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17506935, Length=299, Percent_Identity=41.8060200668896, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=34.6625766871166, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=34.3558282208589, Blast_Score=200, Evalue=3e-52,
Organism=Drosophila melanogaster, GI160714832, Length=327, Percent_Identity=44.6483180428135, Blast_Score=270, Evalue=8e-73,
Organism=Drosophila melanogaster, GI160714828, Length=327, Percent_Identity=44.6483180428135, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI21358145, Length=309, Percent_Identity=36.8932038834951, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24650940, Length=309, Percent_Identity=36.8932038834951, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=39.5604395604396, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=39.5604395604396, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645119, Length=284, Percent_Identity=26.7605633802817, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45551847, Length=284, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550715, Length=284, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36062; Mature: 35931

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGLQQKYGEDRVIDTPLAE
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEECCCCC
SNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFG
CCCHHHHHHHHHHCCCCCHHEEHHHEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
GGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLSSIESNDPVLYFEHKKAYRFL
CCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHH
KEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVY
HHHCCHHHEECCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEC
PLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSM
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
PFSPVLENEIMMNPEKILNKMRELAEF
CCCHHHCHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGLQQKYGEDRVIDTPLAE
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEECCCCC
SNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFG
CCCHHHHHHHHHHCCCCCHHEEHHHEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
GGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLSSIESNDPVLYFEHKKAYRFL
CCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHH
KEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVY
HHHCCHHHEECCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHEECCCCEEEEEEEEEC
PLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSM
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
PFSPVLENEIMMNPEKILNKMRELAEF
CCCHHHCHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]