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The map label for this gene is nudF [H]

Identifier: 57650455

GI number: 57650455

Start: 1579105

End: 1579647

Strand: Reverse

Name: nudF [H]

Synonym: SACOL1542

Alternate gene names: 57650455

Gene position: 1579647-1579105 (Counterclockwise)

Preceding gene: 57650457

Following gene: 57650454

Centisome position: 56.23

GC content: 31.86

Gene sequence:

>543_bases
ATGGATTTAAATGAAAAAACAATTGATCGAACAGTTATTTATAATGGCAAAATTGTAGATGTAGAAATTCATACAGTGAC
ATTACCAAACGGTGAAACGTCAACAAGAGAATTAGTTTATCATAATGGTGCAGTTGCTGTTTGTGCAGTGACACCTAAAA
AAGAAGTCGTATTAGTGAAACAGTATCGTAAACCAGTAGAAAAACCATTGCTAGAAATTCCAGCTGGTAAATTAGAAGAT
GATGAAGATAGAGTCGAAGCGGCAAAACGTGAATTAGAAGAAGAAACAGGATATATTGCCAAAGAGTTAACACATGTTGT
AGATATGTATGGTTCACCAGGTTTTTGCGATGAACAATTATCAATATATTTTACGGATAATTTAGAAGAAGGTACAGTTC
ACTTAGATGAAGATGAATTTGTCGAAGTCATTAAAGTTCCGATTGAGAATGTTAAATCGATGCTAATGAATAAAGAAATA
GAAGATGCGAAGACTATCATTGCATTGCAACACCTATTATTAAATTATAATCATTCTAAATAA

Upstream 100 bases:

>100_bases
TTAATATATTTTTTTGCATGTCTGTTCTCCTTTCGATACACTTTAGCTAACAAGCATAGTGTATCAATATATAATATGAA
TTTAAAGGAACGTGATTATA

Downstream 100 bases:

>100_bases
CAAATGAGAAAAGCTTGCATTTTATTGAGAAAACTGTTAGTTTTAATTGTGAAGTTTGAAATAATTTGTAATGATTTTAA
TTATTAGTAGGGGAGTGGAC

Product: MutT/nudix family protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGAVAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLED
DEDRVEAAKRELEEETGYIAKELTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
EDAKTIIALQHLLLNYNHSK

Sequences:

>Translated_180_residues
MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGAVAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLED
DEDRVEAAKRELEEETGYIAKELTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
EDAKTIIALQHLLLNYNHSK
>Mature_180_residues
MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGAVAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLED
DEDRVEAAKRELEEETGYIAKELTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
EDAKTIIALQHLLLNYNHSK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788810, Length=140, Percent_Identity=33.5714285714286, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1789412, Length=187, Percent_Identity=32.0855614973262, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319587, Length=98, Percent_Identity=36.734693877551, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20439; Mature: 20439

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGAVAVCAVTPKKEVVLVK
CCCCCCCCCEEEEECCEEEEEEEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCEEEHH
QYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKELTHVVDMYGSPGFCDEQL
HHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
SIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEIEDAKTIIALQHLLLNYNHSK
EEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGAVAVCAVTPKKEVVLVK
CCCCCCCCCEEEEECCEEEEEEEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCEEEHH
QYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKELTHVVDMYGSPGFCDEQL
HHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
SIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEIEDAKTIIALQHLLLNYNHSK
EEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]