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The map label for this gene is ypdA [H]

Identifier: 57650434

GI number: 57650434

Start: 1558965

End: 1559951

Strand: Reverse

Name: ypdA [H]

Synonym: SACOL1520

Alternate gene names: 57650434

Gene position: 1559951-1558965 (Counterclockwise)

Preceding gene: 57650435

Following gene: 57650432

Centisome position: 55.53

GC content: 33.64

Gene sequence:

>987_bases
ATGCAAAAAGTTGAAAGTATCATAATTGGTGGAGGGCCATGCGGATTAAGTGCGGCTATTGAACAAAAAAGAAAAGGTAT
TGATACCTTAATTATTGAAAAGGGTAATGTCGTTGAATCAATCTACAATTATCCTACTCACCAAACATTTTTCTCATCAA
GTGATAAATTAAGTATTGGGGACGTACCGTTTATCGTTGAAGAAAGTAAACCAAGACGTAATCAAGCGCTAGTTTATTAC
CGAGAAGTTGTAAAACATCATCAATTAAAAGTAAATGCATTTGAAGAAGTATTAACTGTTAAAAAAATGAATAATAAATT
TACTATTACTACGACGAAAGATGTTTATGAATGTCGATTTTTAACAATCGCGACAGGCTATTATGGTCAGCATAATACAT
TAGAAGTTGAAGGTGCGGATTTACCTAAAGTGTTCCATTATTTTAAAGAGGCACATCCGTATTTTGATCAAGATGTTGTA
ATTATCGGTGGTAAGAATTCGGCTATCGATGCTGCTTTGGAGTTGGAAAAAGCTGGTGCTAACGTGACGGTTCTATATCG
TGGTGGAGATTATTCGCCTTCAATTAAACCGTGGATACTTCCAAATTTCACAGCATTAGTAAATCATGAAAAAATTGACA
TGGAATTTAATGCTAATGTTACCCAAATAACTGAAGATACTGTGACTTATGAAGTAAATGGTGAAAGTAAAACGATACAC
AATGATTATGTATTTGCGATGATTGGTTATCATCCCGATTATGAATTTTTAAAATCTGTAGGCATTCAAATTAATACAAA
TGAATTTGGAACAGCGCCTATGTATAATAAAGAAACATACGAAACAAATATCGAAAATTGCTATATTGCAGGTGTAATTG
CTGCAGGGAACGATGCGAATACCATTTTTATTGAAAATGGTAAATTCCACGGGGGCATTATTGCTCAAAGCATGCTAGCT
AAGAAACAAACGCCCTTAGAATCATAA

Upstream 100 bases:

>100_bases
AAACAAATTGTCAAAAATATATCATAAATCTTCAAGCACAGACTTAGCGCATCAATCACTGAACTGTTATAATAGTTTGG
GATTAAAGGAGGCCGAAACA

Downstream 100 bases:

>100_bases
AAATAAAGGTCTATGTACTAAATAACTTAGTTTTACAACGACTGACATTCATGATATGTCAAATGAGGTTGATGACTAAT
GATTGTACATAGACCTTTTT

Product: pyridine nucleotide-disulfide oxidoreductase

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYY
REVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIH
NDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLA
KKQTPLES

Sequences:

>Translated_328_residues
MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYY
REVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIH
NDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLA
KKQTPLES
>Mature_328_residues
MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYY
REVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIH
NDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLA
KKQTPLES

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 36737; Mature: 36737

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIG
CCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCEEECCCCCEEEC
DVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRF
CCCEEEECCCCCCCCEEHHHHHHHHHHEEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEE
LTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGA
EEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHEEEHHHCCC
NVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIH
CEEEEEECCCCCCCCCCEECCCEEEEECCEEEEEEECCCEEEEECCEEEEEECCCCEEEE
NDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDAN
CCEEEEEEECCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCC
TIFIENGKFHGGIIAQSMLAKKQTPLES
EEEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIG
CCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCCEEECCCCCEEEC
DVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRF
CCCEEEECCCCCCCCEEHHHHHHHHHHEEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEE
LTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGA
EEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHEEEHHHCCC
NVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIH
CEEEEEECCCCCCCCCCEECCCEEEEECCEEEEEEECCCEEEEECCEEEEEECCCCEEEE
NDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDAN
CCEEEEEEECCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCC
TIFIENGKFHGGIIAQSMLAKKQTPLES
EEEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]