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The map label for this gene is sbcD [H]

Identifier: 57650350

GI number: 57650350

Start: 1383605

End: 1384726

Strand: Direct

Name: sbcD [H]

Synonym: SACOL1381

Alternate gene names: 57650350

Gene position: 1383605-1384726 (Clockwise)

Preceding gene: 57650349

Following gene: 57650351

Centisome position: 49.25

GC content: 30.66

Gene sequence:

>1122_bases
ATGAAAATTATACATACAGCAGACTGGCACTTAGGGAAAATATTAAATGGCAAACAGCTTTTAGAAGATCAAGCGTATAT
TTTAGATATGTTCGTAGAAAAAATGAAAGAAGAAGAACCTGATATCATTGTGATAGCTGGAGATTTATATGACACAACAT
ATCCAAGTAAAGATGCAATCATGTTATTAGAACAAGCGATTGGAAAGTTAAATTTAGAACTGCGTATACCAATAATTATT
ATTAGTGGAAATCACGATGGTAAAGAGAGGTTAAACTATGGGGCGAGTTGGTTTGAACATAATCAGTTATTTATAAGAAC
AGATTTTACATCGATTAATTCACCAATAGAGATAAATGGGGTTAATTTTTATACACTCCCTTATGCTACTGTGAGCGAAA
TGAAACACTACTTTGAAGATGACACCATTGAAACACATCAACAGGGAATTACGCGCTGTATTGAAACAATAGCACCGGAA
ATTGATGAAGATGCCGTCAATATTTTAATTAGTCATCTGACTGTTCAAGGTGGAAAGACATCTGATTCTGAAAGACCATT
AACTATTGGAACGGTTGAATCAGTTCAGAAAGGTGTTTTTGATATATTTGATTATGTCATGCTAGGTCACTTGCATCATC
CATTTAGTATAGAAGACGACAAAATTAAATATAGTGGCTCCTTATTGCAGTATTCATTTTCGGAAGCGGGTCAAGCTAAA
GGGTATAGACGTGTAACAATTAATGATGGCATTATTAACGATGTATTTATTCCTCTTAAGCCACTTAGACAATTGGAAAT
TATCTCAGGCGAATATAATGATGTTATTAATGAAAAAGTTCATGTGAAAAATAAAGATAATTATTTACATTTTAAACTTA
AAAATATGTCTCATATTACTGATCCAATGATGAGTTTAAAACAAATTTATCCTAATACTTTAGCGCTGACGAATGAAACT
TTTAATTACAATGAAGAAAATAATGCTATAGAAATAAGTGAAAAAGATGACATGTCAATTATCGAAATGTTTTATAAACA
TATAACTGATAAAGAATTATCGGATATCCAATCTAAAAAGATAAAAAATATTTTAGAAAACGAATTGAGAAAGGAGGATT
AA

Upstream 100 bases:

>100_bases
CGACTTCTAAATCAGTCAATGAGTGGTTTTAATTTTACTTGAAAAGGTGAAATGATTATATTAAAATAAAAGCGAACAAA
TGTTCTATATGGAGGCAGTA

Downstream 100 bases:

>100_bases
TGAATGAAACCATTACATTTAAAGTTGAATAATTTCGGCCCCTTTTTAAAAGAAGAAATTGATTTTTCTAAAATTGATAA
TAATGAATTGTTTTTAATAA

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIVIAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIII
ISGNHDGKERLNYGASWFEHNQLFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIEDDKIKYSGSLLQYSFSEAGQAK
GYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVINEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNET
FNYNEENNAIEISEKDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED

Sequences:

>Translated_373_residues
MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIVIAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIII
ISGNHDGKERLNYGASWFEHNQLFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIEDDKIKYSGSLLQYSFSEAGQAK
GYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVINEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNET
FNYNEENNAIEISEKDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED
>Mature_373_residues
MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIVIAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIII
ISGNHDGKERLNYGASWFEHNQLFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIEDDKIKYSGSLLQYSFSEAGQAK
GYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVINEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNET
FNYNEENNAIEISEKDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=415, Percent_Identity=24.0963855421687, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 42936; Mature: 42936

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIVIAGDLYDTTYPSKDAI
CCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHH
MLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQLFIRTDFTSINSPIEING
HHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHCCCCEEECCEEEEEECCCCCCCCEEECC
VNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPEIDEDAVNILISHLTVQGGKT
EEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCC
SDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIEDDKIKYSGSLLQYSFSEAGQAK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEHHHHHCCC
GYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVINEKVHVKNKDNYLHFKLKNMSHIT
CEEEEEECCCCCCCEECCCCCCCCEEEECCCHHHHCCCEEEEECCCCEEEEEECCCHHHH
DPMMSLKQIYPNTLALTNETFNYNEENNAIEISEKDDMSIIEMFYKHITDKELSDIQSKK
HHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHH
IKNILENELRKED
HHHHHHHHHHCCC
>Mature Secondary Structure
MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIVIAGDLYDTTYPSKDAI
CCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHH
MLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQLFIRTDFTSINSPIEING
HHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHCCCCEEECCEEEEEECCCCCCCCEEECC
VNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPEIDEDAVNILISHLTVQGGKT
EEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCC
SDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIEDDKIKYSGSLLQYSFSEAGQAK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEHHHHHCCC
GYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVINEKVHVKNKDNYLHFKLKNMSHIT
CEEEEEECCCCCCCEECCCCCCCCEEEECCCHHHHCCCEEEEECCCCEEEEEECCCHHHH
DPMMSLKQIYPNTLALTNETFNYNEENNAIEISEKDDMSIIEMFYKHITDKELSDIQSKK
HHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHH
IKNILENELRKED
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA