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The map label for this gene is nucI [H]

Identifier: 57650326

GI number: 57650326

Start: 1363130

End: 1363663

Strand: Direct

Name: nucI [H]

Synonym: SACOL1357

Alternate gene names: 57650326

Gene position: 1363130-1363663 (Clockwise)

Preceding gene: 57650325

Following gene: 57650328

Centisome position: 48.52

GC content: 31.84

Gene sequence:

>534_bases
ATGAAGTCAAATAAATCGCTTGCTATGATTGTGGTAGCCATCATTATTGTAGGTGTATTAGCATTTCAATTTATGAATCA
TACGGGTCCTTTCAAAAAGGGGACGAATCATGAAACTGTACAAGATTTAAATGGTAAAGATAAAGTACATGTTCAAAGAG
TTGTGGATGGTGATACATTTATTGCAAATCAAAATGGTAAAGAAATTAAAGTTAGGCTTATAGGGGTTGATACGCCAGAA
ACGGTGAAACCGAATACGCCTGTACAACCATTTGGCAAAGAAGCATCAAATTATAGTAAGAAGACATTAACAAATCAAGA
TGTTTATTTAGAATATGATAAAGAAAAACAAGATCGCTATGGTAGAACATTGGCGTATGTATGGATAAGTAAAGATCGTA
TGTACAATAAGGAATTAGTGGAAAAGGGACTTGCTAGAGAGAAGTATTTTTCACCAAATGGCAAATATAGAAATGTATTT
ATAGAAGCACAAAATAAAGCTAAACAACAGAAATTAAATATTTGGAGTAAATAA

Upstream 100 bases:

>100_bases
TAGCTTTCCAAAATATTGAAATATGTAGATATGGCTATTGACGATATTTCTTAACTTTTATATGATTAATCGGAATGAAA
AAAGAGAAGTAGGTGGCAAT

Downstream 100 bases:

>100_bases
TTAAATAGGTGATTATATATATTAAAGTAAAATATACTTGAAGCATCGATTTATTATTACCATAAATTTATATATAGAAA
TAATTTAAAGGCATAATAAC

Product: thermonuclease

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPE
TVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
IEAQNKAKQQKLNIWSK

Sequences:

>Translated_177_residues
MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPE
TVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
IEAQNKAKQQKLNIWSK
>Mature_177_residues
MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPE
TVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
IEAQNKAKQQKLNIWSK

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5'-position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 20329; Mature: 20329

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS01284 TNASE_2 ; PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEEEECCCEE
IANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRY
EECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCHHHHC
GRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVFIEAQNKAKQQKLNIWSK
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCC
>Mature Secondary Structure
MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEEEECCCEE
IANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRY
EECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCHHHHC
GRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVFIEAQNKAKQQKLNIWSK
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1408843 [H]