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The map label for this gene is fadD21_2

Identifier: 41408694

GI number: 41408694

Start: 2921621

End: 2923360

Strand: Direct

Name: fadD21_2

Synonym: MAP2596

Alternate gene names: 41408694

Gene position: 2921621-2923360 (Clockwise)

Preceding gene: 41408693

Following gene: 41408698

Centisome position: 60.49

GC content: 69.54

Gene sequence:

>1740_bases
ATGCACGGTTCGTCGGTGGTGTCCCTGATGCGGGAACGTGCCGGCCTGCAACCCGACGATGTGGCGTTCCGCTACACCGA
TTACGAGCAGGACTGGGCGGGCGTCGCCGAGACGCTGACCTGGGCCCAGCTCTATCAGCGGACGTCGAACCTCGCCCGCG
AGGTCGCGCGGCACGGCGACACCGGCGACCGGGCGGTCATTCTCGCGCCCCAGGGCCTGTCCTACATCGTGGCGTTCCTG
GCCGCGATCCAGGCCGGGCTGATCGCGGTTCCGCTGTCGGTGCCCCAGCCCGGTTCGCACGACGAACGGGTCAGCGCCGT
GCTGGCCGACACCGGCCCCGCCGTGGTGCTGACCACCGCCGCGGCCGCCGCGACCGTCACCGAATACCTTCGTCGGCCGG
ACACCGGGCCCGCCCCGGCCGTCGTCGAAATCGACTCGCTGGACCTGGATGAACCGAATTCGTCGAGCATCCGGATCAGC
GCGGCCCCGGACATCGCCTACCTGCAGTACACCTCGGGCTCGACCCGCCTGCCGGCCGGTGTCATGGTCTCGCACCGCAA
CCTGCAGGTGAACTTCCAGCAGCTGATGGCCGCGTTCTTCCCCGACTTCGACGGGGTGGCCCCGCGCAATACGGTCTGCG
TGTCGTGGCTGCCATTCTACCACGACATGGGGCTGGTCCAGGGCGTCATCGCGCCGATCCTGGGCGGCTACCCCGCCGAC
CTCACCAGCCCGGTCGCGTTCCTGCAGCGCCCCGCGCGCTGGATCCAGGCGATGTCGCGGGCGGATCCGGTCTTCTCCGC
GGCGCCCAACTTCGCCTTCGAGCTGGCCGTGCGCAAGACGACCGACGCCGACCTGGCCGGCGTCGACCTGGGCAACATCA
TCAGCATCGTCAGCGGCGCCGAGCGCATCCACCCGGCAACGCTGCGGCGGTTCTGTAAACGCTTCGCGCCGTACAACTTT
CACGAGCACATGATGCAGCCCTCCTACGGCCTGGCCGAGGCGACGGTCTACGTCGCCAGCCGCGCCGAGGCCGGCGGACC
CGAGGTCGTCCACTTCGAGCCGGAAAAGCTGTCGGCCGGCAGCGCGCAGCGGTGCCCGGCGCAGACCGGGTCGCCGCTGC
TGAGCTACGGCGCGCCGACGTCGCCGACGGTGCGGATCGTCGACCCGGACACCAACACCGAACGCGCGGACGGAACGGTC
GGGGAAATCTGGGTCAACGGCGAGAACGTGGCGCAGGGGTATTGGCGCAAACCGGAGGAAACCCGACGCACCTTCGGCGG
CGTGCTGGTCAACCCGTCGCCGGGCACCCCGCGGCAGCCGTGGCTGCGCACCGGCGACCTCGGCTTCATCTCCGCCGGGG
AGTTGTTCATCGTCGGCCGGATGAAGGATTTGCTGATCGTCTACGGCCGCAACCACTATCCCGAGGACATCGAGTCGACG
GTGCAGGCCGTCACCGGCGGCCGGGTCGCGGCCATCTCGGTGCCGGCGGACGAGACCGAGAAATTGGTCACCATCATCGA
GCTGAAGCGGCGCGGCGATTCCGACGAGGACGCGCGGCGCAAGCTCGACGCGGTCAAAAACGAAGTCACCGCGGCGATCT
CGAATTCGCACGGGCTCAACGTCGCGGATCTGGTGCCGGTGGCCCCGGGATCGATTCCCACCACGACCAGCGGCAAGATC
CGGCGGGCGGCCTGCGTCGAGCACTACCTCCGGGGTGCCTTCATCCGGTTGGACGGCTGA

Upstream 100 bases:

>100_bases
ACGCTATTGGCGTGCACCAGTCTAAGGTGTCCCGGTCCGCACGCCCGCCCCGCACGGCGGCGGGCCGGCGCGAAAACCCC
GGAGCGGTAACCTAACCCGC

Downstream 100 bases:

>100_bases
GCGTCCGGCGCTACGCCGGCTCGGTCTCCTCCAGGAAACGGCCCATCACGTCGGGGCCGGTGGAATGCATCGCGATCGAC
AGCTCCAACAGCCGGTCCTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MHGSSVVSLMRERAGLQPDDVAFRYTDYEQDWAGVAETLTWAQLYQRTSNLAREVARHGDTGDRAVILAPQGLSYIVAFL
AAIQAGLIAVPLSVPQPGSHDERVSAVLADTGPAVVLTTAAAAATVTEYLRRPDTGPAPAVVEIDSLDLDEPNSSSIRIS
AAPDIAYLQYTSGSTRLPAGVMVSHRNLQVNFQQLMAAFFPDFDGVAPRNTVCVSWLPFYHDMGLVQGVIAPILGGYPAD
LTSPVAFLQRPARWIQAMSRADPVFSAAPNFAFELAVRKTTDADLAGVDLGNIISIVSGAERIHPATLRRFCKRFAPYNF
HEHMMQPSYGLAEATVYVASRAEAGGPEVVHFEPEKLSAGSAQRCPAQTGSPLLSYGAPTSPTVRIVDPDTNTERADGTV
GEIWVNGENVAQGYWRKPEETRRTFGGVLVNPSPGTPRQPWLRTGDLGFISAGELFIVGRMKDLLIVYGRNHYPEDIEST
VQAVTGGRVAAISVPADETEKLVTIIELKRRGDSDEDARRKLDAVKNEVTAAISNSHGLNVADLVPVAPGSIPTTTSGKI
RRAACVEHYLRGAFIRLDG

Sequences:

>Translated_579_residues
MHGSSVVSLMRERAGLQPDDVAFRYTDYEQDWAGVAETLTWAQLYQRTSNLAREVARHGDTGDRAVILAPQGLSYIVAFL
AAIQAGLIAVPLSVPQPGSHDERVSAVLADTGPAVVLTTAAAAATVTEYLRRPDTGPAPAVVEIDSLDLDEPNSSSIRIS
AAPDIAYLQYTSGSTRLPAGVMVSHRNLQVNFQQLMAAFFPDFDGVAPRNTVCVSWLPFYHDMGLVQGVIAPILGGYPAD
LTSPVAFLQRPARWIQAMSRADPVFSAAPNFAFELAVRKTTDADLAGVDLGNIISIVSGAERIHPATLRRFCKRFAPYNF
HEHMMQPSYGLAEATVYVASRAEAGGPEVVHFEPEKLSAGSAQRCPAQTGSPLLSYGAPTSPTVRIVDPDTNTERADGTV
GEIWVNGENVAQGYWRKPEETRRTFGGVLVNPSPGTPRQPWLRTGDLGFISAGELFIVGRMKDLLIVYGRNHYPEDIEST
VQAVTGGRVAAISVPADETEKLVTIIELKRRGDSDEDARRKLDAVKNEVTAAISNSHGLNVADLVPVAPGSIPTTTSGKI
RRAACVEHYLRGAFIRLDG
>Mature_579_residues
MHGSSVVSLMRERAGLQPDDVAFRYTDYEQDWAGVAETLTWAQLYQRTSNLAREVARHGDTGDRAVILAPQGLSYIVAFL
AAIQAGLIAVPLSVPQPGSHDERVSAVLADTGPAVVLTTAAAAATVTEYLRRPDTGPAPAVVEIDSLDLDEPNSSSIRIS
AAPDIAYLQYTSGSTRLPAGVMVSHRNLQVNFQQLMAAFFPDFDGVAPRNTVCVSWLPFYHDMGLVQGVIAPILGGYPAD
LTSPVAFLQRPARWIQAMSRADPVFSAAPNFAFELAVRKTTDADLAGVDLGNIISIVSGAERIHPATLRRFCKRFAPYNF
HEHMMQPSYGLAEATVYVASRAEAGGPEVVHFEPEKLSAGSAQRCPAQTGSPLLSYGAPTSPTVRIVDPDTNTERADGTV
GEIWVNGENVAQGYWRKPEETRRTFGGVLVNPSPGTPRQPWLRTGDLGFISAGELFIVGRMKDLLIVYGRNHYPEDIEST
VQAVTGGRVAAISVPADETEKLVTIIELKRRGDSDEDARRKLDAVKNEVTAAISNSHGLNVADLVPVAPGSIPTTTSGKI
RRAACVEHYLRGAFIRLDG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=601, Percent_Identity=24.459234608985, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI225735629, Length=604, Percent_Identity=24.8344370860927, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI45827692, Length=604, Percent_Identity=24.8344370860927, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI55749758, Length=545, Percent_Identity=23.6697247706422, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI187761345, Length=567, Percent_Identity=23.8095238095238, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI187761343, Length=567, Percent_Identity=23.8095238095238, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI225735625, Length=586, Percent_Identity=25.4266211604096, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI45827694, Length=530, Percent_Identity=25.8490566037736, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=580, Percent_Identity=25.3448275862069, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=613, Percent_Identity=23.0016313213703, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI193202679, Length=620, Percent_Identity=23.8709677419355, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859661, Length=440, Percent_Identity=25.9090909090909, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24654656, Length=484, Percent_Identity=22.1074380165289, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62365; Mature: 62365

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGSSVVSLMRERAGLQPDDVAFRYTDYEQDWAGVAETLTWAQLYQRTSNLAREVARHGD
CCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGDRAVILAPQGLSYIVAFLAAIQAGLIAVPLSVPQPGSHDERVSAVLADTGPAVVLTTA
CCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHEEEECCCCEEEEEHH
AAAATVTEYLRRPDTGPAPAVVEIDSLDLDEPNSSSIRISAAPDIAYLQYTSGSTRLPAG
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCC
VMVSHRNLQVNFQQLMAAFFPDFDGVAPRNTVCVSWLPFYHDMGLVQGVIAPILGGYPAD
EEEECCCCEEEHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCH
LTSPVAFLQRPARWIQAMSRADPVFSAAPNFAFELAVRKTTDADLAGVDLGNIISIVSGA
HHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCH
ERIHPATLRRFCKRFAPYNFHEHMMQPSYGLAEATVYVASRAEAGGPEVVHFEPEKLSAG
HHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCHHEEEEEEECCCCCCCEEEEECHHHCCCC
SAQRCPAQTGSPLLSYGAPTSPTVRIVDPDTNTERADGTVGEIWVNGENVAQGYWRKPEE
CCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHCCCCCCHH
TRRTFGGVLVNPSPGTPRQPWLRTGDLGFISAGELFIVGRMKDLLIVYGRNHYPEDIEST
HHHHHCCEEECCCCCCCCCCCEEECCCCEEECCCEEEEECCCEEEEEECCCCCCHHHHHH
VQAVTGGRVAAISVPADETEKLVTIIELKRRGDSDEDARRKLDAVKNEVTAAISNSHGLN
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VADLVPVAPGSIPTTTSGKIRRAACVEHYLRGAFIRLDG
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECC
>Mature Secondary Structure
MHGSSVVSLMRERAGLQPDDVAFRYTDYEQDWAGVAETLTWAQLYQRTSNLAREVARHGD
CCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGDRAVILAPQGLSYIVAFLAAIQAGLIAVPLSVPQPGSHDERVSAVLADTGPAVVLTTA
CCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHEEEECCCCEEEEEHH
AAAATVTEYLRRPDTGPAPAVVEIDSLDLDEPNSSSIRISAAPDIAYLQYTSGSTRLPAG
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCC
VMVSHRNLQVNFQQLMAAFFPDFDGVAPRNTVCVSWLPFYHDMGLVQGVIAPILGGYPAD
EEEECCCCEEEHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCH
LTSPVAFLQRPARWIQAMSRADPVFSAAPNFAFELAVRKTTDADLAGVDLGNIISIVSGA
HHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCH
ERIHPATLRRFCKRFAPYNFHEHMMQPSYGLAEATVYVASRAEAGGPEVVHFEPEKLSAG
HHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCHHEEEEEEECCCCCCCEEEEECHHHCCCC
SAQRCPAQTGSPLLSYGAPTSPTVRIVDPDTNTERADGTVGEIWVNGENVAQGYWRKPEE
CCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHCCCCCCHH
TRRTFGGVLVNPSPGTPRQPWLRTGDLGFISAGELFIVGRMKDLLIVYGRNHYPEDIEST
HHHHHCCEEECCCCCCCCCCCEEECCCCEEECCCEEEEECCCEEEEEECCCCCCHHHHHH
VQAVTGGRVAAISVPADETEKLVTIIELKRRGDSDEDARRKLDAVKNEVTAAISNSHGLN
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VADLVPVAPGSIPTTTSGKIRRAACVEHYLRGAFIRLDG
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]