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The map label for this gene is hdhA [C]

Identifier: 41407352

GI number: 41407352

Start: 1333854

End: 1334795

Strand: Direct

Name: hdhA [C]

Synonym: MAP1254

Alternate gene names: 41407352

Gene position: 1333854-1334795 (Clockwise)

Preceding gene: 41407349

Following gene: 41407353

Centisome position: 27.62

GC content: 68.68

Gene sequence:

>942_bases
ATGAACCTTGGTGACCTGACGAACCTGGTGGAAAAGCCCCTCGCGGCGGTCTCCAACATCATCAACACCCCGAACTCGGC
CGGGCGATACCGGCCGCTCTATCTGCGGAACCTGCTGGACGCGGTGCAGGGCCGCACCCTCGAGGAGGCCGTCGACGCCA
AGACCGTGCTGATCACCGGCGCCTCCTCGGGCATCGGTGAGGCCGCCGCCAAGAAGATCGCCGAGGCGGGCGGTGTCGTG
GCGCTGGTGGCCCGCACCCGGGAGAACCTGGAGAAGGTCGCCTCCGAAATCCGGGAGAACGGTGGCAGCGCCCACGTGTA
CCCGTGCGACCTGTCCGACATGGACGCCATCGCCGCGATGGCCGATCGGGTGCTCGACGACCTGGGCGGCGTCGACATCC
TGATCAACAACGCGGGCCGCTCGATCCGGCGCTCGCTGGAGCTGTCCTACGACCGCATCCACGACTACCAGCGCACCATG
CAGTTGAACTACCTGGGCGCGGTGCAGCTCATCCTCAAGTTCATCCCCGGGCATGCGCGAGCGCGGCTTCGGCCAGATCA
TCAACGTGTCGTCGGTCGGGGTGCAGACCCGCGCGCCGCGGTTCGGGGCGTACATCGCCAGCAAGGCCGCGCTGGACAGC
CTGTGCGACGCGCTGCAGGCCGAAGTCGTCAACGACAACGTCAAATTCACCACCGTGCACATGGCGCTGGTGCGCACCCC
GATGATCAGCCCGACCACGTTGTACGACAAGTTCCCGGCGCTGACGCCCGAGCAGGCGGCCGGGGTGATCGCCGACGCGA
TCGTGCACCGGCCGCGGCGGGCCAGCTCGCCGTTCGGGCAGTTCGCCGCCGTCGCCGACGCGGTCAACCCGGCCGTGATG
GACCGGGTGCGCAACCGGGCGTTCGCCATGTTCGGCGACTCCAGCGCCGCCAAGGGCGATGA

Upstream 100 bases:

>100_bases
CGCATGACCGCAATCCGCATCGACGCGGGGCGCTTTTGTCCTAGGTTGGCGTCATGACGCACGTCACATCAGTTGCGCGA
GGGCGGGGGAGCCGAAGCCG

Downstream 100 bases:

>100_bases
ATCCGCAACCGACGCAACCGAATTCGACGCGCGCAGCGAAACGTTCGTGCGGGCGACCCGAGGGATACATTGGTGAGAGG
ATGAGCCTTCCCAAACCCGA

Product: hypothetical protein

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLGDLTNLVEKPLAAVSNIINTPNSAGRYRPLYLRNLLDAVQGRTLEEAVDAKTVLITGASSGIGEAAAKKIAEAGGVV
ALVARTRENLEKVASEIRENGGSAHVYPCDLSDMDAIAAMADRVLDDLGGVDILINNAGRSIRRSLELSYDRIHDYQRTM
QLNYLGAVQLILKFIPGHARARLRPDHQRVVGRGADPRAAVRGVHRQQGRAGQPVRRAAGRSRQRQRQIHHRAHGAGAHP
DDQPDHVVRQVPGADARAGGRGDRRRDRAPAAAGQLAVRAVRRRRRRGQPGRDGPGAQPGVRHVRRLQRRQGR

Sequences:

>Translated_313_residues
MNLGDLTNLVEKPLAAVSNIINTPNSAGRYRPLYLRNLLDAVQGRTLEEAVDAKTVLITGASSGIGEAAAKKIAEAGGVV
ALVARTRENLEKVASEIRENGGSAHVYPCDLSDMDAIAAMADRVLDDLGGVDILINNAGRSIRRSLELSYDRIHDYQRTM
QLNYLGAVQLILKFIPGHARARLRPDHQRVVGRGADPRAAVRGVHRQQGRAGQPVRRAAGRSRQRQRQIHHRAHGAGAHP
DDQPDHVVRQVPGADARAGGRGDRRRDRAPAAAGQLAVRAVRRRRRRGQPGRDGPGAQPGVRHVRRLQRRQGR
>Mature_313_residues
MNLGDLTNLVEKPLAAVSNIINTPNSAGRYRPLYLRNLLDAVQGRTLEEAVDAKTVLITGASSGIGEAAAKKIAEAGGVV
ALVARTRENLEKVASEIRENGGSAHVYPCDLSDMDAIAAMADRVLDDLGGVDILINNAGRSIRRSLELSYDRIHDYQRTM
QLNYLGAVQLILKFIPGHARARLRPDHQRVVGRGADPRAAVRGVHRQQGRAGQPVRRAAGRSRQRQRQIHHRAHGAGAHP
DDQPDHVVRQVPGADARAGGRGDRRRDRAPAAAGQLAVRAVRRRRRRGQPGRDGPGAQPGVRHVRRLQRRQGR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7706318, Length=146, Percent_Identity=37.6712328767123, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI32455239, Length=129, Percent_Identity=35.6589147286822, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI5031765, Length=129, Percent_Identity=35.6589147286822, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI20149619, Length=204, Percent_Identity=29.9019607843137, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787905, Length=120, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=128, Percent_Identity=34.375, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71997402, Length=178, Percent_Identity=32.0224719101124, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21358495, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007236, Length=114, Percent_Identity=41.2280701754386, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI116007238, Length=114, Percent_Identity=41.2280701754386, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364892, Length=172, Percent_Identity=29.0697674418605, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007232, Length=84, Percent_Identity=40.4761904761905, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007234, Length=84, Percent_Identity=40.4761904761905, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 34139; Mature: 34139

Theoretical pI: Translated: 11.97; Mature: 11.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLGDLTNLVEKPLAAVSNIINTPNSAGRYRPLYLRNLLDAVQGRTLEEAVDAKTVLITG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCEEEEEC
ASSGIGEAAAKKIAEAGGVVALVARTRENLEKVASEIRENGGSAHVYPCDLSDMDAIAAM
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
ADRVLDDLGGVDILINNAGRSIRRSLELSYDRIHDYQRTMQLNYLGAVQLILKFIPGHAR
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARLRPDHQRVVGRGADPRAAVRGVHRQQGRAGQPVRRAAGRSRQRQRQIHHRAHGAGAHP
HCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DDQPDHVVRQVPGADARAGGRGDRRRDRAPAAAGQLAVRAVRRRRRRGQPGRDGPGAQPG
CCCHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
VRHVRRLQRRQGR
HHHHHHHHHHCCC
>Mature Secondary Structure
MNLGDLTNLVEKPLAAVSNIINTPNSAGRYRPLYLRNLLDAVQGRTLEEAVDAKTVLITG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCEEEEEC
ASSGIGEAAAKKIAEAGGVVALVARTRENLEKVASEIRENGGSAHVYPCDLSDMDAIAAM
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
ADRVLDDLGGVDILINNAGRSIRRSLELSYDRIHDYQRTMQLNYLGAVQLILKFIPGHAR
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARLRPDHQRVVGRGADPRAAVRGVHRQQGRAGQPVRRAAGRSRQRQRQIHHRAHGAGAHP
HCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DDQPDHVVRQVPGADARAGGRGDRRRDRAPAAAGQLAVRAVRRRRRRGQPGRDGPGAQPG
CCCHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
VRHVRRLQRRQGR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]