Click here to switch to the map view.

The map label for this gene is epiA

Identifier: 41407330

GI number: 41407330

Start: 1294481

End: 1295443

Strand: Direct

Name: epiA

Synonym: MAP1232

Alternate gene names: 41407330

Gene position: 1294481-1295443 (Clockwise)

Preceding gene: 41407329

Following gene: 41407331

Centisome position: 26.8

GC content: 58.88

Gene sequence:

>963_bases
TTGACGACTACACCTGGGCCTCTGGACCGCGCAACGCCCGTGTATATCGCCGGTCATCGGGGGCTGGTCGGCTCAGCGCT
CGTACGTAGATTTGAGGCCGAGGGGTTCACCAATCTCATTGTGCGATCACGCGATGAGATTGATCTGACGGACCGAGCCG
CAACGTTTGATTTTGTGTCTGAGACAAGACCACAGGTGATCATCGATGCGGCCGCACGGGTCGGCGGCATCATGGCGAAT
AACACCTATCCCGCGGACTTCTTGTCCGAAAACCTCCGAATCCAGACCAATTTGCTCGACGCAGCTGTCGCCGTGCGTGT
GCCGCGGCTCCTTTTCCTCGGTTCGTCATGCATCTACCCGAAGTACGCTCCGCAACCTATCCACGAGAGTGCTTTATTGA
CTGGCCCTTTGGAGCCCACCAACGACGCGTATGCGATCGCCAAGATCGCCGGTATCCTGCAAGTTCAGGCGGTTAGGCGC
CAATATGGGCTGGCGTGGATCTCTGCGATGCCGACTAACCTCTACGGACCCGGCGACAACTTCTCCCCGTCCGGGTCGCA
TCTCTTGCCGGCGCTCATCCGTCGATATGAGGAAGCCAAAGCTGGTGGTGCAGAAGAGGTGACGAATTGGGGGACCGGTA
CTCCGCGGCGCGAACTTCTGCATGTCGACGATCTGGCGAGCGCATGCCTGTTCCTTTTGGAACATTTCGATGGTCCGAAC
CACGTCAACGTGGGCACCGGCGTCGATCACAGCATTAGCGAGATCGCAGACATGGTCGCTACGGCGGTGGGCTACATCGG
CGAAACACGTTGGGATCCAACTAAACCCGATGGAACCCCGCGCAAACTATTGGACGTCTCCGCGCTACGCGAGTTGGGTT
GGCGCCCGCGAATCGCACTGAAAGAGGGCATCGATGCAACGGTGTCGTGGTACCGCACAAATGCCGATGCCGTGAGGAGG
TAA

Upstream 100 bases:

>100_bases
AACTCGCGCGCATCATGGTGGACGCGGACATCGCCGCGTTGGAGTGCGATGGCACACCATGGATCGACACGCCGATGTTG
CCTGGTTGGGGCAGAGTAAG

Downstream 100 bases:

>100_bases
AGCTGCGGGCCGGCCGATGTTATCCCTCCGGCCGGACGGGTAGGGCGACCTGCCATCGAGTGGTACGGCAGTCGCCTGGC
CGGCGAGGCGCATGGCCTAT

Product: EpiA

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTTTPGPLDRATPVYIAGHRGLVGSALVRRFEAEGFTNLIVRSRDEIDLTDRAATFDFVSETRPQVIIDAAARVGGIMAN
NTYPADFLSENLRIQTNLLDAAVAVRVPRLLFLGSSCIYPKYAPQPIHESALLTGPLEPTNDAYAIAKIAGILQVQAVRR
QYGLAWISAMPTNLYGPGDNFSPSGSHLLPALIRRYEEAKAGGAEEVTNWGTGTPRRELLHVDDLASACLFLLEHFDGPN
HVNVGTGVDHSISEIADMVATAVGYIGETRWDPTKPDGTPRKLLDVSALRELGWRPRIALKEGIDATVSWYRTNADAVRR

Sequences:

>Translated_320_residues
MTTTPGPLDRATPVYIAGHRGLVGSALVRRFEAEGFTNLIVRSRDEIDLTDRAATFDFVSETRPQVIIDAAARVGGIMAN
NTYPADFLSENLRIQTNLLDAAVAVRVPRLLFLGSSCIYPKYAPQPIHESALLTGPLEPTNDAYAIAKIAGILQVQAVRR
QYGLAWISAMPTNLYGPGDNFSPSGSHLLPALIRRYEEAKAGGAEEVTNWGTGTPRRELLHVDDLASACLFLLEHFDGPN
HVNVGTGVDHSISEIADMVATAVGYIGETRWDPTKPDGTPRKLLDVSALRELGWRPRIALKEGIDATVSWYRTNADAVRR
>Mature_319_residues
TTTPGPLDRATPVYIAGHRGLVGSALVRRFEAEGFTNLIVRSRDEIDLTDRAATFDFVSETRPQVIIDAAARVGGIMANN
TYPADFLSENLRIQTNLLDAAVAVRVPRLLFLGSSCIYPKYAPQPIHESALLTGPLEPTNDAYAIAKIAGILQVQAVRRQ
YGLAWISAMPTNLYGPGDNFSPSGSHLLPALIRRYEEAKAGGAEEVTNWGTGTPRRELLHVDDLASACLFLLEHFDGPNH
VNVGTGVDHSISEIADMVATAVGYIGETRWDPTKPDGTPRKLLDVSALRELGWRPRIALKEGIDATVSWYRTNADAVRR

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=314, Percent_Identity=32.8025477707006, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI7657641, Length=227, Percent_Identity=25.5506607929515, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788365, Length=315, Percent_Identity=53.3333333333333, Blast_Score=340, Evalue=7e-95,
Organism=Caenorhabditis elegans, GI17554428, Length=314, Percent_Identity=26.4331210191083, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922778, Length=321, Percent_Identity=31.7757009345794, Blast_Score=147, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 34885; Mature: 34754

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPGPLDRATPVYIAGHRGLVGSALVRRFEAEGFTNLIVRSRDEIDLTDRAATFDFVS
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHH
ETRPQVIIDAAARVGGIMANNTYPADFLSENLRIQTNLLDAAVAVRVPRLLFLGSSCIYP
CCCCCEEEEHHHHHCCEEECCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCC
KYAPQPIHESALLTGPLEPTNDAYAIAKIAGILQVQAVRRQYGLAWISAMPTNLYGPGDN
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCC
FSPSGSHLLPALIRRYEEAKAGGAEEVTNWGTGTPRRELLHVDDLASACLFLLEHFDGPN
CCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
HVNVGTGVDHSISEIADMVATAVGYIGETRWDPTKPDGTPRKLLDVSALRELGWRPRIAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
KEGIDATVSWYRTNADAVRR
HCCCCCEEHEEECCHHHHCC
>Mature Secondary Structure 
TTTPGPLDRATPVYIAGHRGLVGSALVRRFEAEGFTNLIVRSRDEIDLTDRAATFDFVS
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHH
ETRPQVIIDAAARVGGIMANNTYPADFLSENLRIQTNLLDAAVAVRVPRLLFLGSSCIYP
CCCCCEEEEHHHHHCCEEECCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCC
KYAPQPIHESALLTGPLEPTNDAYAIAKIAGILQVQAVRRQYGLAWISAMPTNLYGPGDN
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCC
FSPSGSHLLPALIRRYEEAKAGGAEEVTNWGTGTPRRELLHVDDLASACLFLLEHFDGPN
CCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
HVNVGTGVDHSISEIADMVATAVGYIGETRWDPTKPDGTPRKLLDVSALRELGWRPRIAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
KEGIDATVSWYRTNADAVRR
HCCCCCEEHEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]