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The map label for this gene is gmdA

Identifier: 41407329

GI number: 41407329

Start: 1293453

End: 1294484

Strand: Direct

Name: gmdA

Synonym: MAP1231

Alternate gene names: 41407329

Gene position: 1293453-1294484 (Clockwise)

Preceding gene: 41407328

Following gene: 41407330

Centisome position: 26.78

GC content: 59.69

Gene sequence:

>1032_bases
GTGAAGCGAGCGCTTATAACAGGGATCACGGGGCAGGATGGTTCCTACCTCGCCGAGCTACTACTGAGCAAGGGATACGA
GGTTCACGGGCTCGTTCGTCGAGCTTCGACGTTTAACACGTCGCGGATCGATCACCTCTACGTTGACCCACACCAACCGG
GCGCGCGCTTGTTCTTGCACTATGCAGACCTCACTGACGGCACCCGGTTGGTGACCCTGCTCAGCAGTATCGACCCGGAT
GAGGTCTACAACCTCGCAGCGCAGTCCCATGTGCGCGTCAGCTTTGACGAGCCAGTGCATACCGGAGACACCACCGGCAT
GGGATCGATCCGACTTCTGGAAGCAGTCCGCCTTTCTCGGGTGGACTGCCGGTTCTATCAGGCTTCCTCGTCGGAGATGT
TCGGCGCATCTCCGCCACCGCAGAACGAATCGACGCCGTTCTATCCCCGTTCGCCATACGGCGCGGCCAAGGTCTTCTCG
TACTGGACGACTCGCAACTATCGAGAGGCGTACGGATTATTCGCAGTGAATGGCATCTTGTTCAACCATGAGTCCCCCCG
GCGCGGCGAGACTTTCGTGACCCGAAAGATCACGCGTGCCGTGGCGCGCATCCGAGCTGGCGTCCAATCGGAGGTCTATA
TGGGCAACCTCGATGCGATCCGCGACTGGGGCTACGCGCCCGAATATGTCGAGGGGATGTGGAGGATGTTGCAAGCGCCT
GAACCTGATGACTACGTCCTGGCGACAGGGCGTGGTTACACCGTACGTGAGTTCGCTCAAGCTGCTTTTGACCACGTCGG
GCTCGACTGGCAAAAGCACGTCAAGTTTGACGACCGCTATTTGCGCCCCACCGAGGTCGATTCGCTAGTAGGAGATGCCG
ACAGGGCGGCCCAGTCACTCGGCTGGAAAGCTTCGGTTCATACTGGTGAACTCGCGCGCATCATGGTGGACGCGGACATC
GCCGCGTTGGAGTGCGATGGCACACCATGGATCGACACGCCGATGTTGCCTGGTTGGGGCAGAGTAAGTTGA

Upstream 100 bases:

>100_bases
ACGATTCGGATCCCTTGCACCACAACGAGAGTGAGACCGCCATGATGACGAAATATCGGCTGGGCGGAGTCAACGCCGGA
GTGACAAAAGTGAGAACCCG

Downstream 100 bases:

>100_bases
CGACTACACCTGGGCCTCTGGACCGCGCAACGCCCGTGTATATCGCCGGTCATCGGGGGCTGGTCGGCTCAGCGCTCGTA
CGTAGATTTGAGGCCGAGGG

Product: GmdA

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKRALITGITGQDGSYLAELLLSKGYEVHGLVRRASTFNTSRIDHLYVDPHQPGARLFLHYADLTDGTRLVTLLSSIDPD
EVYNLAAQSHVRVSFDEPVHTGDTTGMGSIRLLEAVRLSRVDCRFYQASSSEMFGASPPPQNESTPFYPRSPYGAAKVFS
YWTTRNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAVARIRAGVQSEVYMGNLDAIRDWGYAPEYVEGMWRMLQAP
EPDDYVLATGRGYTVREFAQAAFDHVGLDWQKHVKFDDRYLRPTEVDSLVGDADRAAQSLGWKASVHTGELARIMVDADI
AALECDGTPWIDTPMLPGWGRVS

Sequences:

>Translated_343_residues
MKRALITGITGQDGSYLAELLLSKGYEVHGLVRRASTFNTSRIDHLYVDPHQPGARLFLHYADLTDGTRLVTLLSSIDPD
EVYNLAAQSHVRVSFDEPVHTGDTTGMGSIRLLEAVRLSRVDCRFYQASSSEMFGASPPPQNESTPFYPRSPYGAAKVFS
YWTTRNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAVARIRAGVQSEVYMGNLDAIRDWGYAPEYVEGMWRMLQAP
EPDDYVLATGRGYTVREFAQAAFDHVGLDWQKHVKFDDRYLRPTEVDSLVGDADRAAQSLGWKASVHTGELARIMVDADI
AALECDGTPWIDTPMLPGWGRVS
>Mature_343_residues
MKRALITGITGQDGSYLAELLLSKGYEVHGLVRRASTFNTSRIDHLYVDPHQPGARLFLHYADLTDGTRLVTLLSSIDPD
EVYNLAAQSHVRVSFDEPVHTGDTTGMGSIRLLEAVRLSRVDCRFYQASSSEMFGASPPPQNESTPFYPRSPYGAAKVFS
YWTTRNYREAYGLFAVNGILFNHESPRRGETFVTRKITRAVARIRAGVQSEVYMGNLDAIRDWGYAPEYVEGMWRMLQAP
EPDDYVLATGRGYTVREFAQAAFDHVGLDWQKHVKFDDRYLRPTEVDSLVGDADRAAQSLGWKASVHTGELARIMVDADI
AALECDGTPWIDTPMLPGWGRVS

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=55.0724637681159, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=51.2820512820513, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI48994969, Length=245, Percent_Identity=28.1632653061224, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788353, Length=201, Percent_Identity=28.3582089552239, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=346, Percent_Identity=54.9132947976879, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI133901790, Length=346, Percent_Identity=54.9132947976879, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI133901786, Length=346, Percent_Identity=54.9132947976879, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17539422, Length=346, Percent_Identity=54.9132947976879, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI133901788, Length=346, Percent_Identity=54.9132947976879, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17507723, Length=346, Percent_Identity=54.9132947976879, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=53.3527696793003, Blast_Score=372, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38409; Mature: 38409

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRALITGITGQDGSYLAELLLSKGYEVHGLVRRASTFNTSRIDHLYVDPHQPGARLFLH
CCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEE
YADLTDGTRLVTLLSSIDPDEVYNLAAQSHVRVSFDEPVHTGDTTGMGSIRLLEAVRLSR
EECCCCCHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
VDCRFYQASSSEMFGASPPPQNESTPFYPRSPYGAAKVFSYWTTRNYREAYGLFAVNGIL
HHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCEEEEEEEE
FNHESPRRGETFVTRKITRAVARIRAGVQSEVYMGNLDAIRDWGYAPEYVEGMWRMLQAP
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHCCC
EPDDYVLATGRGYTVREFAQAAFDHVGLDWQKHVKFDDRYLRPTEVDSLVGDADRAAQSL
CCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHC
GWKASVHTGELARIMVDADIAALECDGTPWIDTPMLPGWGRVS
CCEEECCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRALITGITGQDGSYLAELLLSKGYEVHGLVRRASTFNTSRIDHLYVDPHQPGARLFLH
CCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEE
YADLTDGTRLVTLLSSIDPDEVYNLAAQSHVRVSFDEPVHTGDTTGMGSIRLLEAVRLSR
EECCCCCHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
VDCRFYQASSSEMFGASPPPQNESTPFYPRSPYGAAKVFSYWTTRNYREAYGLFAVNGIL
HHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCEEEEEEEE
FNHESPRRGETFVTRKITRAVARIRAGVQSEVYMGNLDAIRDWGYAPEYVEGMWRMLQAP
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHCCC
EPDDYVLATGRGYTVREFAQAAFDHVGLDWQKHVKFDDRYLRPTEVDSLVGDADRAAQSL
CCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHC
GWKASVHTGELARIMVDADIAALECDGTPWIDTPMLPGWGRVS
CCEEECCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]