Click here to switch to the map view.

The map label for this gene is metB

Identifier: 41407124

GI number: 41407124

Start: 1071753

End: 1072919

Strand: Direct

Name: metB

Synonym: MAP1026

Alternate gene names: 41407124

Gene position: 1071753-1072919 (Clockwise)

Preceding gene: 41407123

Following gene: 41407127

Centisome position: 22.19

GC content: 71.12

Gene sequence:

>1167_bases
ATGAGCGACGACGGCAACGCACACCCCGAGTTCAGCGGACTGGCCACCAAGGCGATTCACGCCGGCTACCGGCCGGACCC
GGCGACCGGAGCGGTCAACACGCCGATCTACGCCAGCAGCACCTTCGCCCAGGACGGGGTGGGCGCGCTGCGCGGCGGGT
ACGAGTACGCCCGCACCGGCAACCCGACCCGGGCCGCGCTGGAGGCCACGCTGGCCGCGGTGGAGGCCGGCAGCCACGGG
CGGGCCTTCGCCTCCGGCATGGCCGCCACCGACTGCGCGCTGCGGGCGGTGCTGCGGCCCGGCGACCATGTGGTGATCCC
GAATGACGCCTACGGCGGCACCTTTCGGCTGATCGACAAGGTGTTCACTCGCTGGAACGTCGAATACACGCCGGTGCCGT
TGCATCAGCTCGACGCCGTGGCCGCCGCGATCACGCCGCGCACCCGGCTGATCTGGGTGGAGACCCCGACCAACCCGTTG
CTGTCGATCGCCGACATCGCCGCGCTGGCCCAGCTGGGCCACCAGCACTCGGCAAAGGTGTTGGTGGACAACACCTTTGC
CTCGCCGGCGCTGCAGCAGCCGTTGACGCTGGGCGCCGACATCGTGCTGCATTCCACCACCAAGTACATCGGCGGGCACT
CCGACGTGGTGGGCGGTGCGCTGGTCACCAACGACGCCGAGCTGGACGACGCGTTCGGGTTCTTGCAGAACGGGGCGGGC
GCGGTGCCCGGCCCGTTCGACGCCTACCTGACCATGCGCGGGCTGAAGACCCTGGTGCTGCGGATGCAGCGGCACACCGA
AAACGCCTCTGCCGTAGCGCAATTCCTCGCCGGGCACCCCGCGGTGAGCGCGGTGCTCTATCCCGGCCTGCCCGGCCATC
CGGGCCACGACGTCGCCGCCCGGCAGATGTCGGGCTTCGGCGGCATGGTCTCGGTGCGGATGCGCGGCGGCCGGCAGGCC
GCCCGAAAGCTGTGCGCCAACACCCGGGTGTTCATCCTGGCCGAGTCGCTGGGCGGGGTCGAGTCGCTGATCGAGCACCC
CAGCGCGATGACGCACGCGTCGACGGCGGGGTCGCAACTGGAGGTGCCCGACGATCTGGTGCGGCTGTCGGTCGGCATCG
AGGACGTCGACGACCTGCTGGCCGACCTCGACCGGGCGCTCAGTTGA

Upstream 100 bases:

>100_bases
GCGGACAAGATCATGACGACCGTGTGCCTGCCGCTCGACGGCTCCGAAAGCCCGCCGAGCTAGGGCCGGCCACCGCGGCC
GTCCCCTAGGCTGATCGTCG

Downstream 100 bases:

>100_bases
TACGCCGAGCGCTCGGACTCAGGAGTGGTAGGGCTCCGCGCTGACCAGCGTGACCTCGACGGTGCTGCCGTTGGGCACGG
TGTAGCTGCGGGTCTCGCCC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSDDGNAHPEFSGLATKAIHAGYRPDPATGAVNTPIYASSTFAQDGVGALRGGYEYARTGNPTRAALEATLAAVEAGSHG
RAFASGMAATDCALRAVLRPGDHVVIPNDAYGGTFRLIDKVFTRWNVEYTPVPLHQLDAVAAAITPRTRLIWVETPTNPL
LSIADIAALAQLGHQHSAKVLVDNTFASPALQQPLTLGADIVLHSTTKYIGGHSDVVGGALVTNDAELDDAFGFLQNGAG
AVPGPFDAYLTMRGLKTLVLRMQRHTENASAVAQFLAGHPAVSAVLYPGLPGHPGHDVAARQMSGFGGMVSVRMRGGRQA
ARKLCANTRVFILAESLGGVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDVDDLLADLDRALS

Sequences:

>Translated_388_residues
MSDDGNAHPEFSGLATKAIHAGYRPDPATGAVNTPIYASSTFAQDGVGALRGGYEYARTGNPTRAALEATLAAVEAGSHG
RAFASGMAATDCALRAVLRPGDHVVIPNDAYGGTFRLIDKVFTRWNVEYTPVPLHQLDAVAAAITPRTRLIWVETPTNPL
LSIADIAALAQLGHQHSAKVLVDNTFASPALQQPLTLGADIVLHSTTKYIGGHSDVVGGALVTNDAELDDAFGFLQNGAG
AVPGPFDAYLTMRGLKTLVLRMQRHTENASAVAQFLAGHPAVSAVLYPGLPGHPGHDVAARQMSGFGGMVSVRMRGGRQA
ARKLCANTRVFILAESLGGVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDVDDLLADLDRALS
>Mature_387_residues
SDDGNAHPEFSGLATKAIHAGYRPDPATGAVNTPIYASSTFAQDGVGALRGGYEYARTGNPTRAALEATLAAVEAGSHGR
AFASGMAATDCALRAVLRPGDHVVIPNDAYGGTFRLIDKVFTRWNVEYTPVPLHQLDAVAAAITPRTRLIWVETPTNPLL
SIADIAALAQLGHQHSAKVLVDNTFASPALQQPLTLGADIVLHSTTKYIGGHSDVVGGALVTNDAELDDAFGFLQNGAGA
VPGPFDAYLTMRGLKTLVLRMQRHTENASAVAQFLAGHPAVSAVLYPGLPGHPGHDVAARQMSGFGGMVSVRMRGGRQAA
RKLCANTRVFILAESLGGVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDVDDLLADLDRALS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=369, Percent_Identity=47.1544715447154, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI299473759, Length=369, Percent_Identity=43.0894308943089, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI25453487, Length=368, Percent_Identity=41.304347826087, Blast_Score=265, Evalue=4e-71,
Organism=Escherichia coli, GI1790375, Length=373, Percent_Identity=42.627345844504, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=31.6326530612245, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=371, Percent_Identity=45.2830188679245, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17538055, Length=371, Percent_Identity=46.6307277628032, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI71980708, Length=379, Percent_Identity=38.7862796833773, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319307, Length=389, Percent_Identity=48.586118251928, Blast_Score=326, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6321254, Length=399, Percent_Identity=31.077694235589, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=30.1149425287356, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=334, Percent_Identity=30.2395209580838, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=29.3670886075949, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=48.1675392670157, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40591; Mature: 40460

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDGNAHPEFSGLATKAIHAGYRPDPATGAVNTPIYASSTFAQDGVGALRGGYEYARTG
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCHHHCCC
NPTRAALEATLAAVEAGSHGRAFASGMAATDCALRAVLRPGDHVVIPNDAYGGTFRLIDK
CCHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
VFTRWNVEYTPVPLHQLDAVAAAITPRTRLIWVETPTNPLLSIADIAALAQLGHQHSAKV
HHHHCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
LVDNTFASPALQQPLTLGADIVLHSTTKYIGGHSDVVGGALVTNDAELDDAFGFLQNGAG
EEECCCCCHHHHCCHHCCCEEEEECCHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCC
AVPGPFDAYLTMRGLKTLVLRMQRHTENASAVAQFLAGHPAVSAVLYPGLPGHPGHDVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHEEEEECCCCCCCCCHHHH
RQMSGFGGMVSVRMRGGRQAARKLCANTRVFILAESLGGVESLIEHPSAMTHASTAGSQL
HHHCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECHHCCHHHHHHCCCHHHHHHCCCCCC
EVPDDLVRLSVGIEDVDDLLADLDRALS
CCCHHHHEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDDGNAHPEFSGLATKAIHAGYRPDPATGAVNTPIYASSTFAQDGVGALRGGYEYARTG
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCHHHCCC
NPTRAALEATLAAVEAGSHGRAFASGMAATDCALRAVLRPGDHVVIPNDAYGGTFRLIDK
CCHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
VFTRWNVEYTPVPLHQLDAVAAAITPRTRLIWVETPTNPLLSIADIAALAQLGHQHSAKV
HHHHCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
LVDNTFASPALQQPLTLGADIVLHSTTKYIGGHSDVVGGALVTNDAELDDAFGFLQNGAG
EEECCCCCHHHHCCHHCCCEEEEECCHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCC
AVPGPFDAYLTMRGLKTLVLRMQRHTENASAVAQFLAGHPAVSAVLYPGLPGHPGHDVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHEEEEECCCCCCCCCHHHH
RQMSGFGGMVSVRMRGGRQAARKLCANTRVFILAESLGGVESLIEHPSAMTHASTAGSQL
HHHCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECHHCCHHHHHHCCCHHHHHHCCCCCC
EVPDDLVRLSVGIEDVDDLLADLDRALS
CCCHHHHEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]