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The map label for this gene is nodT [H]

Identifier: 52842722

GI number: 52842722

Start: 2841752

End: 2843314

Strand: Reverse

Name: nodT [H]

Synonym: lpg2514

Alternate gene names: 52842722

Gene position: 2843314-2841752 (Counterclockwise)

Preceding gene: 52842723

Following gene: 52842721

Centisome position: 83.68

GC content: 41.71

Gene sequence:

>1563_bases
CTGGTATACATGTTGAAAATCCTTGCTCTCTTAAGTTGTTTGATTCTAAGCTCCTGTCTGGTCGGGCCAAATTTTAAAGA
GCCTAAAAAAGATGCGGCCAATTATTGGCTTACTTCAAGCCCTGCAATAAAACAATCCGCTTTTCAAAATGCGAATTGGT
GGAAAGTCTTTAATGATCCCACTTTAACCTCATTAATCGATAACGGTTATCACAACAATCTTTCCTTGCAAATGGCAGGA
GTACGAGTTTTGCAAGCAAGAGCTCAACTTGCTCAATCAGTTGGGGAGCTTTATCCCCAGCAACAAGCTATGGTAGGAGA
TTATAATTACAATCGAATTGGGGGCAATCAATTACAAGGGCTTCTACCGCCCGATTTTTTAACAGCCAGCCTCGGATTTT
CCGCAAGCTGGGAACTTGATTTTTGGGGAAAATACCGAAGAGCGATTCAATCTAATGATGCCCTGTTCCTTGCCTCTCTG
GCAGCTTATGATTCCGCATTGGTTACTCTCACCTCAGATATTGCCAGTACTTTTATTAAAATCCGCACCAGCGAAGAATT
AATCAAAGTAACAAAAACTAATATCCAGGTGCAGGCGATGAGTCTTAAAATCGCAAAGGATCGTTATTATGGTGGTCAAA
CCAGTTTACTGGATGTGGAACAAGCGGCAACAGAACTTGCGGAAACTCAATCAAAACTGCCCCAATACGTGAGTGACCTC
CAGCACCAAAAAGATATTTTGGCTGTATTACTTGGTACCACTCCTGATAAGGTGGATGACTTGCTTAAAAAAAATAAAGG
AATACCAAAAGCTTTAGCGGTAGTGGAAGTTGGAATTCCTAAAGAAGTGATAGCGCGAAGGCCAGATGTTTATCAAGCCA
GAATGGAGGCTATAGCCCAATCAGCTGCAATTGGCGCTGTGAAGGCTAATCTTTATCCTTCACTTTCATTAGTGGGGACT
TTTGTTTTTGCCTCAAATAATATTGGCAATTCTACTATTAGTGATATATTTAACTGGTCTAATCGCAATATAGTCGCAGG
CCCGTCACTGGCATGGTCTTTATTAAATTATGGTCAAATCACGAACGCGGTACGAGCTCAAGACGCTGCTTTCCAGCAAG
CATTGCTGAACTATCTTAATTTGGTGCTCAAAGTGCAGCAGGAAGTTCAAGATAACATTACGCGCTATGTAGAGGGGCAA
AAAACCGTCTCTTCACTTAAAACAGCCAATGCTGCTGCCATAGAATCGACAAGACTTGCTTTAGTACGATACAAGGAAGG
AGAGGCTAATTACACCACTGTATTGAATGCAGAACAGCAACAATTACGAGTACAAACTTCACTGGTTAATGCCACAGGAG
AATTGGCTTTGTCTCTGGTTGGCTTATACCGCTCGCTTGGAGGCGGTTGGCAAATACGCGGCAAGAATGATATTGTTTCA
AATCAAATAAAAAGTGAAATGGCTGCCCGTACTAATTGGGGTAGTTTATTGAAGCAAGAAAATCATTTGCCTCCAGACTC
TAACGGACAACGAATGAAACAACTATATTTACCAACCTGGTGA

Upstream 100 bases:

>100_bases
TTCAATTGAGTATTCATAGATAATATCTAATGATTGATGAACCAATAGTTCAAATCCATCTTCAATCTACATAAGCTGCT
TTTGAACAGATAGGGAAATA

Downstream 100 bases:

>100_bases
TGAGAATGAACTCTCAAAATAAACAAAATTTAGTTAAAACCGTAGCAATTGTATTTTTTGGTATTGTGTTTGTTTATTTA
GTATATGACCATTTTACAAG

Product: outer membrane efflux protein (RND multidrug efflux)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MVYMLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSAFQNANWWKVFNDPTLTSLIDNGYHNNLSLQMAG
VRVLQARAQLAQSVGELYPQQQAMVGDYNYNRIGGNQLQGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASL
AAYDSALVTLTSDIASTFIKIRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQAATELAETQSKLPQYVSDL
QHQKDILAVLLGTTPDKVDDLLKKNKGIPKALAVVEVGIPKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGT
FVFASNNIGNSTISDIFNWSNRNIVAGPSLAWSLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITRYVEGQ
KTVSSLKTANAAAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATGELALSLVGLYRSLGGGWQIRGKNDIVS
NQIKSEMAARTNWGSLLKQENHLPPDSNGQRMKQLYLPTW

Sequences:

>Translated_520_residues
MVYMLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSAFQNANWWKVFNDPTLTSLIDNGYHNNLSLQMAG
VRVLQARAQLAQSVGELYPQQQAMVGDYNYNRIGGNQLQGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASL
AAYDSALVTLTSDIASTFIKIRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQAATELAETQSKLPQYVSDL
QHQKDILAVLLGTTPDKVDDLLKKNKGIPKALAVVEVGIPKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGT
FVFASNNIGNSTISDIFNWSNRNIVAGPSLAWSLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITRYVEGQ
KTVSSLKTANAAAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATGELALSLVGLYRSLGGGWQIRGKNDIVS
NQIKSEMAARTNWGSLLKQENHLPPDSNGQRMKQLYLPTW
>Mature_520_residues
MVYMLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSAFQNANWWKVFNDPTLTSLIDNGYHNNLSLQMAG
VRVLQARAQLAQSVGELYPQQQAMVGDYNYNRIGGNQLQGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASL
AAYDSALVTLTSDIASTFIKIRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQAATELAETQSKLPQYVSDL
QHQKDILAVLLGTTPDKVDDLLKKNKGIPKALAVVEVGIPKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGT
FVFASNNIGNSTISDIFNWSNRNIVAGPSLAWSLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITRYVEGQ
KTVSSLKTANAAAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATGELALSLVGLYRSLGGGWQIRGKNDIVS
NQIKSEMAARTNWGSLLKQENHLPPDSNGQRMKQLYLPTW

Specific function: In Pathogenic Strains It Allows The Bacteria To Invade Brain Microvascular Endothelial Cells (Bmec) Thus Allowing It To Cross The Blood-Brain Barrier And Cause Neonatal Meningitis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=478, Percent_Identity=23.8493723849372, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI2367348, Length=493, Percent_Identity=23.7322515212982, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 57303; Mature: 57303

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYMLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSAFQNANWWKVFNDP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEECCCCHHHHHHHCCCCCEEEECCC
TLTSLIDNGYHNNLSLQMAGVRVLQARAQLAQSVGELYPQQQAMVGDYNYNRIGGNQLQG
HHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCC
LLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASLAAYDSALVTLTSDIASTFIK
CCCHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEE
IRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQAATELAETQSKLPQYVSDL
EECHHHHHHHHHCCEEEEEEEEEEEHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QHQKDILAVLLGTTPDKVDDLLKKNKGIPKALAVVEVGIPKEVIARRPDVYQARMEAIAQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHEEECCCCHHHHHCCCHHHHHHHHHHHH
SAAIGAVKANLYPSLSLVGTFVFASNNIGNSTISDIFNWSNRNIVAGPSLAWSLLNYGQI
HHHHHHHHHCCCCCHHHHEEEEEECCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHH
TNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITRYVEGQKTVSSLKTANAAAIESTRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHEEE
LVRYKEGEANYTTVLNAEQQQLRVQTSLVNATGELALSLVGLYRSLGGGWQIRGKNDIVS
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHH
NQIKSEMAARTNWGSLLKQENHLPPDSNGQRMKQLYLPTW
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MVYMLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSAFQNANWWKVFNDP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEECCCCHHHHHHHCCCCCEEEECCC
TLTSLIDNGYHNNLSLQMAGVRVLQARAQLAQSVGELYPQQQAMVGDYNYNRIGGNQLQG
HHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCC
LLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASLAAYDSALVTLTSDIASTFIK
CCCHHHHHHHCCCCEEECCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEE
IRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQAATELAETQSKLPQYVSDL
EECHHHHHHHHHCCEEEEEEEEEEEHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QHQKDILAVLLGTTPDKVDDLLKKNKGIPKALAVVEVGIPKEVIARRPDVYQARMEAIAQ
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHEEECCCCHHHHHCCCHHHHHHHHHHHH
SAAIGAVKANLYPSLSLVGTFVFASNNIGNSTISDIFNWSNRNIVAGPSLAWSLLNYGQI
HHHHHHHHHCCCCCHHHHEEEEEECCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHH
TNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITRYVEGQKTVSSLKTANAAAIESTRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHEEE
LVRYKEGEANYTTVLNAEQQQLRVQTSLVNATGELALSLVGLYRSLGGGWQIRGKNDIVS
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHH
NQIKSEMAARTNWGSLLKQENHLPPDSNGQRMKQLYLPTW
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2338917; 2622339 [H]