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The map label for this gene is yitJ [H]

Identifier: 39998065

GI number: 39998065

Start: 3265450

End: 3267267

Strand: Reverse

Name: yitJ [H]

Synonym: GSU2974

Alternate gene names: 39998065

Gene position: 3267267-3265450 (Counterclockwise)

Preceding gene: 39998066

Following gene: 39998064

Centisome position: 85.66

GC content: 66.39

Gene sequence:

>1818_bases
ATGACCTTTCTCGAACGGCTGCATGACGAGGTCCTCATCGGCGACGGAGCCATCGGCACCATGCTTTATGCCAAGGGGGT
ATCCCTCGACGCCAACGTTGAGCACCTGAACCTGGTGCGCCCGTCGCTGGTGCTGGAACTCCACCGGGAGTACCTGGCGG
CCGGGGCCCGCGTTATCGAAACCAACACCTTCGGCGCCAACTGGACCCGGCTCGCGGCCATCGGTCTTGAGAAGAAGGAG
CGTGAGATCAACCTCCGGGGTGCCCAACTGGCCCGGGAGGCCGCCCAGGGCACCGACGCCTTCGTGGCCGGCTCCGTGGG
GCCCCTGGTCCGGATGAAGGGGGACGAACAGGAACTCTCCGCCCAAGAAACCGTCGACATCTTCCGGAGGCAGACCCATG
CCCTGGCCGAGGGGGAGGTGGATCTCCTGATCCTGGAGACCTTCACCGATCTGGCGCAGATGAGGCACGCCCTGACCGCG
GCCCGGGAAACGGGGCTGCCGGTGGTGGCCAACATGGCGTTCCTGGAAAACGGGAGACTCGCCGGCGGGATCGAGGTGGA
GCGGGTAGCCGTGGAGCTGACTGCCGCCGGTGCCTGCGTGGTGGGCGCCAACTGCGGCGCCGGGCCATTGGAAATCCTCG
GCACCATCCAGCGGATGGGCCGGGTGACGGATCTTCCCCTGGCGGCCTATCCCAACAGCGGATTTCCCGAATACGTGAAC
GGCCGCCATGTCTACCGGACCACCCCTGATTACTTCGCGGACCGGGCCGTGGAGATGGTCGGGGCGGGAGCGGCACTGGT
GGGGGGATGCTGCGGCACCACGCCGGAGCACGTGCGGTGCCTGGCGCAGCGGCTGGCGGGGCTGCGGCCCGCGGCACGCA
CGGTTGCCGTTTCCGCCCTGCCCAAGGATGAGCGCAGACCGGAGTCCCGCGAGTATGCCGGCTTCCTGGCCCGCTGGGGA
CGTGAGCCCATTGTCACCGTGGAGCTGGATCCGCCCAAGGGGCTCGACTGCGCGAAGATCATCGAGGGGAGCAGGGCGCT
GCGGGAGGCTGGGGCGGACGCCATCAACATCGCCGAGAACCCGCTGGCCCGGATCCGGATGGGTAACATCGCCCTGGGGA
GCCTCATTCAGCGGGAGGCGGGAATCGACGTCATTGTTCACGTGACCTGCCGGGACCGCAACCTGCTGGGGCTCCAGTCG
GACCTCATGGGGGCGAGCCTGTTGGGGATTCGCCACGTGCTGGCCGTCACCGGTGATCCCGCCCGTATCGGCGAACAGGC
CGGGGCCACATCGGTGTACGATCTGAATTCCTTCGGCCTCATCAAGCTGCTGGCCGATTTGAACGCCGGAGTGAACGCTC
TGGGCTCGCCCATAGGCGGAGGGACGGGGTTCGTCATCGGAGCCGCTTTCAATCCCAACAGCGCCCGGATGGAGGTCCAG
GTGGAGCGCTTGGCCAGGAAGGTGGCCAACGGCGCCCGTTTTGTCCAAACCCAGCCCCTCTATGATGTGGCGCGGCTGGA
GGAGATGGCGGAGCGGACGGCGCACCTGGGTATCCCCATCCTGCCCGGAATTCTGCCACTGGTGAGCGAGCGCAACTGCG
AATTTCTCCATAACGAGGTGCCGGGCATCGTGATCCCCGAAGAAATCCGGCACCGGATGCGGGGAAAGGAAAAAGAGGAC
GGAGTGAGGGAAGGCCTCGCCATCGCCCGGGAGTTCATTGATGCGGTGCGGGACCGGGTGGGCGGATTCTACCTCATCCC
TCCCTTCGGCAAATACGGTATTGCCGTGGAGTTGACGCGGTACATCAAGGGGACCTGA

Upstream 100 bases:

>100_bases
ATCCCCAAGTGGAGCCTCACCTCTATGAGCTCAAGGGGGTCATGTCCCGCAAGAAGCAGATGGTTCCCCACTTGCTCGGG
GTGCTCGGGAAGGTGCAGGC

Downstream 100 bases:

>100_bases
TCCCCAGCGAGGCTGCCCATGAACGTTCGTCGAAACCTGTCACTTCTCCTCCTGGCCCTTGTCCTTGCCGGCTGCCGCAA
CCCCTACCTCATTGCTCAGC

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MTFLERLHDEVLIGDGAIGTMLYAKGVSLDANVEHLNLVRPSLVLELHREYLAAGARVIETNTFGANWTRLAAIGLEKKE
REINLRGAQLAREAAQGTDAFVAGSVGPLVRMKGDEQELSAQETVDIFRRQTHALAEGEVDLLILETFTDLAQMRHALTA
ARETGLPVVANMAFLENGRLAGGIEVERVAVELTAAGACVVGANCGAGPLEILGTIQRMGRVTDLPLAAYPNSGFPEYVN
GRHVYRTTPDYFADRAVEMVGAGAALVGGCCGTTPEHVRCLAQRLAGLRPAARTVAVSALPKDERRPESREYAGFLARWG
REPIVTVELDPPKGLDCAKIIEGSRALREAGADAINIAENPLARIRMGNIALGSLIQREAGIDVIVHVTCRDRNLLGLQS
DLMGASLLGIRHVLAVTGDPARIGEQAGATSVYDLNSFGLIKLLADLNAGVNALGSPIGGGTGFVIGAAFNPNSARMEVQ
VERLARKVANGARFVQTQPLYDVARLEEMAERTAHLGIPILPGILPLVSERNCEFLHNEVPGIVIPEEIRHRMRGKEKED
GVREGLAIAREFIDAVRDRVGGFYLIPPFGKYGIAVELTRYIKGT

Sequences:

>Translated_605_residues
MTFLERLHDEVLIGDGAIGTMLYAKGVSLDANVEHLNLVRPSLVLELHREYLAAGARVIETNTFGANWTRLAAIGLEKKE
REINLRGAQLAREAAQGTDAFVAGSVGPLVRMKGDEQELSAQETVDIFRRQTHALAEGEVDLLILETFTDLAQMRHALTA
ARETGLPVVANMAFLENGRLAGGIEVERVAVELTAAGACVVGANCGAGPLEILGTIQRMGRVTDLPLAAYPNSGFPEYVN
GRHVYRTTPDYFADRAVEMVGAGAALVGGCCGTTPEHVRCLAQRLAGLRPAARTVAVSALPKDERRPESREYAGFLARWG
REPIVTVELDPPKGLDCAKIIEGSRALREAGADAINIAENPLARIRMGNIALGSLIQREAGIDVIVHVTCRDRNLLGLQS
DLMGASLLGIRHVLAVTGDPARIGEQAGATSVYDLNSFGLIKLLADLNAGVNALGSPIGGGTGFVIGAAFNPNSARMEVQ
VERLARKVANGARFVQTQPLYDVARLEEMAERTAHLGIPILPGILPLVSERNCEFLHNEVPGIVIPEEIRHRMRGKEKED
GVREGLAIAREFIDAVRDRVGGFYLIPPFGKYGIAVELTRYIKGT
>Mature_604_residues
TFLERLHDEVLIGDGAIGTMLYAKGVSLDANVEHLNLVRPSLVLELHREYLAAGARVIETNTFGANWTRLAAIGLEKKER
EINLRGAQLAREAAQGTDAFVAGSVGPLVRMKGDEQELSAQETVDIFRRQTHALAEGEVDLLILETFTDLAQMRHALTAA
RETGLPVVANMAFLENGRLAGGIEVERVAVELTAAGACVVGANCGAGPLEILGTIQRMGRVTDLPLAAYPNSGFPEYVNG
RHVYRTTPDYFADRAVEMVGAGAALVGGCCGTTPEHVRCLAQRLAGLRPAARTVAVSALPKDERRPESREYAGFLARWGR
EPIVTVELDPPKGLDCAKIIEGSRALREAGADAINIAENPLARIRMGNIALGSLIQREAGIDVIVHVTCRDRNLLGLQSD
LMGASLLGIRHVLAVTGDPARIGEQAGATSVYDLNSFGLIKLLADLNAGVNALGSPIGGGTGFVIGAAFNPNSARMEVQV
ERLARKVANGARFVQTQPLYDVARLEEMAERTAHLGIPILPGILPLVSERNCEFLHNEVPGIVIPEEIRHRMRGKEKEDG
VREGLAIAREFIDAVRDRVGGFYLIPPFGKYGIAVELTRYIKGT

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=355, Percent_Identity=27.887323943662, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI13162290, Length=312, Percent_Identity=31.7307692307692, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI157266337, Length=314, Percent_Identity=32.1656050955414, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1790450, Length=323, Percent_Identity=29.7213622291022, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786456, Length=299, Percent_Identity=29.7658862876254, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=355, Percent_Identity=29.5774647887324, Blast_Score=135, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6322966, Length=308, Percent_Identity=23.7012987012987, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 65049; Mature: 64917

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLERLHDEVLIGDGAIGTMLYAKGVSLDANVEHLNLVRPSLVLELHREYLAAGARVIE
CCHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
TNTFGANWTRLAAIGLEKKEREINLRGAQLAREAAQGTDAFVAGSVGPLVRMKGDEQELS
ECCCCCCCCEEEEECCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCHHHHH
AQETVDIFRRQTHALAEGEVDLLILETFTDLAQMRHALTAARETGLPVVANMAFLENGRL
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCC
AGGIEVERVAVELTAAGACVVGANCGAGPLEILGTIQRMGRVTDLPLAAYPNSGFPEYVN
CCCCEEEEEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
GRHVYRTTPDYFADRAVEMVGAGAALVGGCCGTTPEHVRCLAQRLAGLRPAARTVAVSAL
CCEEEECCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHEEEECC
PKDERRPESREYAGFLARWGREPIVTVELDPPKGLDCAKIIEGSRALREAGADAINIAEN
CCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCC
PLARIRMGNIALGSLIQREAGIDVIVHVTCRDRNLLGLQSDLMGASLLGIRHVLAVTGDP
CHHHEEECCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
ARIGEQAGATSVYDLNSFGLIKLLADLNAGVNALGSPIGGGTGFVIGAAFNPNSARMEVQ
HHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCCCEEEHH
VERLARKVANGARFVQTQPLYDVARLEEMAERTAHLGIPILPGILPLVSERNCEFLHNEV
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCC
PGIVIPEEIRHRMRGKEKEDGVREGLAIAREFIDAVRDRVGGFYLIPPFGKYGIAVELTR
CCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEH
YIKGT
HHCCC
>Mature Secondary Structure 
TFLERLHDEVLIGDGAIGTMLYAKGVSLDANVEHLNLVRPSLVLELHREYLAAGARVIE
CHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
TNTFGANWTRLAAIGLEKKEREINLRGAQLAREAAQGTDAFVAGSVGPLVRMKGDEQELS
ECCCCCCCCEEEEECCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCEEEECCCHHHHH
AQETVDIFRRQTHALAEGEVDLLILETFTDLAQMRHALTAARETGLPVVANMAFLENGRL
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCC
AGGIEVERVAVELTAAGACVVGANCGAGPLEILGTIQRMGRVTDLPLAAYPNSGFPEYVN
CCCCEEEEEEEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
GRHVYRTTPDYFADRAVEMVGAGAALVGGCCGTTPEHVRCLAQRLAGLRPAARTVAVSAL
CCEEEECCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHEEEECC
PKDERRPESREYAGFLARWGREPIVTVELDPPKGLDCAKIIEGSRALREAGADAINIAEN
CCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCC
PLARIRMGNIALGSLIQREAGIDVIVHVTCRDRNLLGLQSDLMGASLLGIRHVLAVTGDP
CHHHEEECCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
ARIGEQAGATSVYDLNSFGLIKLLADLNAGVNALGSPIGGGTGFVIGAAFNPNSARMEVQ
HHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCCCEEEHH
VERLARKVANGARFVQTQPLYDVARLEEMAERTAHLGIPILPGILPLVSERNCEFLHNEV
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCC
PGIVIPEEIRHRMRGKEKEDGVREGLAIAREFIDAVRDRVGGFYLIPPFGKYGIAVELTR
CCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEH
YIKGT
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]