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The map label for this gene is rpoA

Identifier: 39997923

GI number: 39997923

Start: 3115118

End: 3116140

Strand: Reverse

Name: rpoA

Synonym: GSU2831

Alternate gene names: 39997923

Gene position: 3116140-3115118 (Counterclockwise)

Preceding gene: 39997924

Following gene: 39997922

Centisome position: 81.7

GC content: 52.39

Gene sequence:

>1023_bases
ATGTATAGAAACTGGCGCGACCTGATCAGTCCGAAGAAACTTCAGGTTGAAAGCGAGACGCTTACCAATAAATATGGAAA
ATTTTATGCAGAACCGTTTGAGCGCGGATTCGGCACGACTCTCGGCAACTCACTTCGCAGGGTCTTGCTTTCTTCGCTCC
AAGGTGCAGGAATTACCTCTGTAAGGATCAAGGGGGTGCTTCACGAATTTTCTTCCATCCCCGGCGTAACCGAGGATGTT
ACCAATATCATTCTCAACCTTAAGGGCGTCAGCCTCAAAATGTACGGTACTGAGCCTAAGACCGTCCGGATTATCCATAA
GGGTGACGGTATTATCACGGCAGGTGATATCATTACCGACCCCCAAGTCGAGATCCTGAATCCGGAACACCACATCGCCA
CATGCTCCAAGGATGCAAATCTTGAAATGGAGATGGTGGTGAAGGTTGGCAAGGGCTATGTGCCCGCAGACCGGAATCGC
GATGAGAAGGCTCCCGTTGGCACGATTCCGATTGATGCCCTGTTTTCACCGATCCGCAAGGTTAACTTTACGGTTTCAAA
CGCCCGCGTCGGTCAGATGACCGATTATGACAAGCTGACGCTTGAGGTGTGGACCAACGGCAGCGTGATCCCGGAAGACG
CGGTCGCCTTTGCCGCCAAGATCCTCAAGGAACAACTGAGCATTTTCATTAACTTTGACGAGGAAGCTGAACCGAGCGGC
GAGGCTGAAGTTGGCGAAGGGGAAAGCCCCATCAACGAGAACCTCTATCGTTCGGTCGATGAACTCGAGCTTTCCGTGCG
CTCTGCTAACTGCCTCAAGAACGCAGGCATTAAGCTTATTGGCGAGCTTGTGTCCCGGACCGAGGCCGAGATGCTCAAAA
CGCAGAACTTCGGCCGCAAGTCGTTGAACGAGATCAAGGATATACTCGCTGAGATGGGCCTTACGCTTGGTATGAAACTG
GAAGGGTTCCCCGATCCGGAAGTCATGCGCAGGCTGCGCGGCGAGCGCAAGGATGAAGAATAA

Upstream 100 bases:

>100_bases
TTCCCGTGCGTGAAGACATTACGATGCCGATCCAGGAGCAACTGATCGTCGAGCTCTATTCGAAGTAATGTTCGCATAAC
CCGTCACAGGGAGGATATAC

Downstream 100 bases:

>100_bases
TTCGTACGGTTGCCGGAAGAAAGGAACATAGGTCATGCGTCACAATAAGGCGGGAAGAAGGCTGGGAAGAACGACGAGTC
ACCGTATCGCCATGTTCAGG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MYRNWRDLISPKKLQVESETLTNKYGKFYAEPFERGFGTTLGNSLRRVLLSSLQGAGITSVRIKGVLHEFSSIPGVTEDV
TNIILNLKGVSLKMYGTEPKTVRIIHKGDGIITAGDIITDPQVEILNPEHHIATCSKDANLEMEMVVKVGKGYVPADRNR
DEKAPVGTIPIDALFSPIRKVNFTVSNARVGQMTDYDKLTLEVWTNGSVIPEDAVAFAAKILKEQLSIFINFDEEAEPSG
EAEVGEGESPINENLYRSVDELELSVRSANCLKNAGIKLIGELVSRTEAEMLKTQNFGRKSLNEIKDILAEMGLTLGMKL
EGFPDPEVMRRLRGERKDEE

Sequences:

>Translated_340_residues
MYRNWRDLISPKKLQVESETLTNKYGKFYAEPFERGFGTTLGNSLRRVLLSSLQGAGITSVRIKGVLHEFSSIPGVTEDV
TNIILNLKGVSLKMYGTEPKTVRIIHKGDGIITAGDIITDPQVEILNPEHHIATCSKDANLEMEMVVKVGKGYVPADRNR
DEKAPVGTIPIDALFSPIRKVNFTVSNARVGQMTDYDKLTLEVWTNGSVIPEDAVAFAAKILKEQLSIFINFDEEAEPSG
EAEVGEGESPINENLYRSVDELELSVRSANCLKNAGIKLIGELVSRTEAEMLKTQNFGRKSLNEIKDILAEMGLTLGMKL
EGFPDPEVMRRLRGERKDEE
>Mature_340_residues
MYRNWRDLISPKKLQVESETLTNKYGKFYAEPFERGFGTTLGNSLRRVLLSSLQGAGITSVRIKGVLHEFSSIPGVTEDV
TNIILNLKGVSLKMYGTEPKTVRIIHKGDGIITAGDIITDPQVEILNPEHHIATCSKDANLEMEMVVKVGKGYVPADRNR
DEKAPVGTIPIDALFSPIRKVNFTVSNARVGQMTDYDKLTLEVWTNGSVIPEDAVAFAAKILKEQLSIFINFDEEAEPSG
EAEVGEGESPINENLYRSVDELELSVRSANCLKNAGIKLIGELVSRTEAEMLKTQNFGRKSLNEIKDILAEMGLTLGMKL
EGFPDPEVMRRLRGERKDEE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=313, Percent_Identity=46.3258785942492, Blast_Score=281, Evalue=4e-77,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_GEOSL (Q749B3)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953874.1
- ProteinModelPortal:   Q749B3
- GeneID:   2686836
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2831
- NMPDR:   fig|243231.1.peg.2812
- TIGR:   GSU2831
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   GSUL243231:GSU_2831-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37727; Mature: 37727

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRNWRDLISPKKLQVESETLTNKYGKFYAEPFERGFGTTLGNSLRRVLLSSLQGAGITS
CCCCHHHHCCCCCEEEHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHCCCCCCCH
VRIKGVLHEFSSIPGVTEDVTNIILNLKGVSLKMYGTEPKTVRIIHKGDGIITAGDIITD
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCCC
PQVEILNPEHHIATCSKDANLEMEMVVKVGKGYVPADRNRDEKAPVGTIPIDALFSPIRK
CCEEEECCCHHEEECCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VNFTVSNARVGQMTDYDKLTLEVWTNGSVIPEDAVAFAAKILKEQLSIFINFDEEAEPSG
HEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCC
EAEVGEGESPINENLYRSVDELELSVRSANCLKNAGIKLIGELVSRTEAEMLKTQNFGRK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
SLNEIKDILAEMGLTLGMKLEGFPDPEVMRRLRGERKDEE
HHHHHHHHHHHCCCEECCEECCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYRNWRDLISPKKLQVESETLTNKYGKFYAEPFERGFGTTLGNSLRRVLLSSLQGAGITS
CCCCHHHHCCCCCEEEHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHCCCCCCCH
VRIKGVLHEFSSIPGVTEDVTNIILNLKGVSLKMYGTEPKTVRIIHKGDGIITAGDIITD
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCCC
PQVEILNPEHHIATCSKDANLEMEMVVKVGKGYVPADRNRDEKAPVGTIPIDALFSPIRK
CCEEEECCCHHEEECCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VNFTVSNARVGQMTDYDKLTLEVWTNGSVIPEDAVAFAAKILKEQLSIFINFDEEAEPSG
HEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCC
EAEVGEGESPINENLYRSVDELELSVRSANCLKNAGIKLIGELVSRTEAEMLKTQNFGRK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
SLNEIKDILAEMGLTLGMKLEGFPDPEVMRRLRGERKDEE
HHHHHHHHHHHCCCEECCEECCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA