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The map label for this gene is nifD [H]

Identifier: 39997913

GI number: 39997913

Start: 3102400

End: 3103839

Strand: Reverse

Name: nifD [H]

Synonym: GSU2820

Alternate gene names: 39997913

Gene position: 3103839-3102400 (Counterclockwise)

Preceding gene: 39997914

Following gene: 39997912

Centisome position: 81.38

GC content: 60.9

Gene sequence:

>1440_bases
ATGTCCAATGCAGCCAAAAGAGTTGAAGGAATAACCAAAGAGTCGACTCAGGAGATGATCGACAAGGTGTTGGAGGTTTA
CCCGGAAAAAGGGCGTAAGAAGCGCGCTCCACACCTGGCCCCCAACGACGGCGTCAGTTCCTGCGTAAAGTCCAACCGCA
AGACCGTTCCCGGCGTCATGAGCGCCCGGGGCTGCGCCTACGCCGGCGCCAAGGGGGTCGTCTGGGGCCCCATCCGCGAC
ATGGTTCACATCTCCCATGGTCCGGTGGGGTGCGGCTGGTACTCCTGGGGTACTCGCCGCAACCTGATGAGCGGCATCAA
CGGGGTCACCAACTTCGGCATGCAGTTCACGTCGGATTTCCAGGAAAAGGATATCGTCTACGGCGGGGACAAGAAGTTGA
AGCAACTGCTGGAGGAGGCCAAGGGACTGTTCCCGCTGGCCAAGGGGATTTCGGTCCTGTCCGAGTGTCCGGTGGGCCTC
ATCGGCGACGACATCAACGCCGTGGCCAAGCAGTCCGCCAAGGAGCTGGACATCCCGGTCATACCCTGTAACTGCGAAGG
GTTCCGCGGGGTCAGCCAGTCCCTGGGCCACCACATTTCCAACGACACCATCCGCGACTTCATCATCGGCACCCGGGAGT
TTGCCGAGCCCGAGAGCCCGTACGACATCGCCCTTATCGGCGACTACAACATCGGCGGTGACGTCTGGAGCGCCAAGGCG
CTGCTGGAGGAGATCGGCCTCAACGTGAAGGCCACCTGGACCGGCGACGGCGAGTTGGACCGGATCGCCGCCACCCACAC
GGTGAAGCTCAACCTCATCCACTGCTACCGCTCCATGAACTACATGTGCCGCGTGATGGAAGAGAAGTACGGCATCCCCT
GGCTGGAGTTCAACTTCTTCGGCCCCAGCAAGATCAAGGAGAGCCTGCGCGCGATTGGTGAGCGGTTCGACGACAGGATC
AAGGAGAACGTGGAAAAGGTCATTGCCAAGTACGACCCGATCATGCAGGCGGTGATCGACGAGTATCGGCCGCGGCTCGA
AGGGAAGAAGGTCATGATCTACGTCGGCGGTCTCCGTCCCCGCCACACCGTGGGCGCCTACGAGGACCTGGGGATGGTGG
TTGTCGGCACCGGCTACGAGTTCGCCCACGGCGACGACTACGAGCGGACCTCCCCCGAGATGCCGGTCGATACGGTCATT
TTTGACGATGCCTCCGAGTTCGAGCTGGAGAAGTTCGCCCATCAGATCAAGCCCGACCTGGTTGCCTCCGGCATCAAAGA
GAAGTACGTGTTCCAGAAAATGGGGCTTCCCTTCCGCCAGATGCACAGCTGGGACTACTCCGGTCCCTATCACGGCTACC
AGGGATTCCCGATCTTTGCCCGGGACATTGACATGGCGGTGAATAGCCCCACGTGGTCGCTGATCAAGTCGCCGTTCTAG

Upstream 100 bases:

>100_bases
TACCCACCCCCTCACCGGGCCGGCCTCAAAATCGTTCGCCAACGGTTCCGCGCAACCAGGTAAGGGCGCACACTGTGCGG
CCTTTGACAGGAGAATACTT

Downstream 100 bases:

>100_bases
ACGGAGTCCGCTTTTCCGCCATCTCGGCGTGCCTTCGTCATCGTTTGTGCGGCCTCCGCGCTCTTCATCGGCGCCACGGT
CCTGACGAAAATCGGCGTCG

Product: nitrogenase molybdenum-iron protein, alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSNAAKRVEGITKESTQEMIDKVLEVYPEKGRKKRAPHLAPNDGVSSCVKSNRKTVPGVMSARGCAYAGAKGVVWGPIRD
MVHISHGPVGCGWYSWGTRRNLMSGINGVTNFGMQFTSDFQEKDIVYGGDKKLKQLLEEAKGLFPLAKGISVLSECPVGL
IGDDINAVAKQSAKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDFIIGTREFAEPESPYDIALIGDYNIGGDVWSAKA
LLEEIGLNVKATWTGDGELDRIAATHTVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFFGPSKIKESLRAIGERFDDRI
KENVEKVIAKYDPIMQAVIDEYRPRLEGKKVMIYVGGLRPRHTVGAYEDLGMVVVGTGYEFAHGDDYERTSPEMPVDTVI
FDDASEFELEKFAHQIKPDLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYQGFPIFARDIDMAVNSPTWSLIKSPF

Sequences:

>Translated_479_residues
MSNAAKRVEGITKESTQEMIDKVLEVYPEKGRKKRAPHLAPNDGVSSCVKSNRKTVPGVMSARGCAYAGAKGVVWGPIRD
MVHISHGPVGCGWYSWGTRRNLMSGINGVTNFGMQFTSDFQEKDIVYGGDKKLKQLLEEAKGLFPLAKGISVLSECPVGL
IGDDINAVAKQSAKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDFIIGTREFAEPESPYDIALIGDYNIGGDVWSAKA
LLEEIGLNVKATWTGDGELDRIAATHTVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFFGPSKIKESLRAIGERFDDRI
KENVEKVIAKYDPIMQAVIDEYRPRLEGKKVMIYVGGLRPRHTVGAYEDLGMVVVGTGYEFAHGDDYERTSPEMPVDTVI
FDDASEFELEKFAHQIKPDLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYQGFPIFARDIDMAVNSPTWSLIKSPF
>Mature_478_residues
SNAAKRVEGITKESTQEMIDKVLEVYPEKGRKKRAPHLAPNDGVSSCVKSNRKTVPGVMSARGCAYAGAKGVVWGPIRDM
VHISHGPVGCGWYSWGTRRNLMSGINGVTNFGMQFTSDFQEKDIVYGGDKKLKQLLEEAKGLFPLAKGISVLSECPVGLI
GDDINAVAKQSAKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDFIIGTREFAEPESPYDIALIGDYNIGGDVWSAKAL
LEEIGLNVKATWTGDGELDRIAATHTVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFFGPSKIKESLRAIGERFDDRIK
ENVEKVIAKYDPIMQAVIDEYRPRLEGKKVMIYVGGLRPRHTVGAYEDLGMVVVGTGYEFAHGDDYERTSPEMPVDTVIF
DDASEFELEKFAHQIKPDLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYQGFPIFARDIDMAVNSPTWSLIKSPF

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 53490; Mature: 53359

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00435 PEROXIDASE_1 ; PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAAKRVEGITKESTQEMIDKVLEVYPEKGRKKRAPHLAPNDGVSSCVKSNRKTVPGVM
CCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHH
SARGCAYAGAKGVVWGPIRDMVHISHGPVGCGWYSWGTRRNLMSGINGVTNFGMQFTSDF
HCCCCCCCCCCCCEECCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCC
QEKDIVYGGDKKLKQLLEEAKGLFPLAKGISVLSECPVGLIGDDINAVAKQSAKELDIPV
CCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCE
IPCNCEGFRGVSQSLGHHISNDTIRDFIIGTREFAEPESPYDIALIGDYNIGGDVWSAKA
EECCCCCCCHHHHHHHHHCCCCHHHHHHHCCHHHCCCCCCCEEEEEECCCCCCHHHHHHH
LLEEIGLNVKATWTGDGELDRIAATHTVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFF
HHHHHCCCEEEEECCCCCHHHEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GPSKIKESLRAIGERFDDRIKENVEKVIAKYDPIMQAVIDEYRPRLEGKKVMIYVGGLRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RHTVGAYEDLGMVVVGTGYEFAHGDDYERTSPEMPVDTVIFDDASEFELEKFAHQIKPDL
CCCCCCHHCCCEEEEECCEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCHHH
VASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYQGFPIFARDIDMAVNSPTWSLIKSPF
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure 
SNAAKRVEGITKESTQEMIDKVLEVYPEKGRKKRAPHLAPNDGVSSCVKSNRKTVPGVM
CCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHH
SARGCAYAGAKGVVWGPIRDMVHISHGPVGCGWYSWGTRRNLMSGINGVTNFGMQFTSDF
HCCCCCCCCCCCCEECCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCC
QEKDIVYGGDKKLKQLLEEAKGLFPLAKGISVLSECPVGLIGDDINAVAKQSAKELDIPV
CCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCE
IPCNCEGFRGVSQSLGHHISNDTIRDFIIGTREFAEPESPYDIALIGDYNIGGDVWSAKA
EECCCCCCCHHHHHHHHHCCCCHHHHHHHCCHHHCCCCCCCEEEEEECCCCCCHHHHHHH
LLEEIGLNVKATWTGDGELDRIAATHTVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFF
HHHHHCCCEEEEECCCCCHHHEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GPSKIKESLRAIGERFDDRIKENVEKVIAKYDPIMQAVIDEYRPRLEGKKVMIYVGGLRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RHTVGAYEDLGMVVVGTGYEFAHGDDYERTSPEMPVDTVIFDDASEFELEKFAHQIKPDL
CCCCCCHHCCCEEEEECCEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCHHH
VASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYQGFPIFARDIDMAVNSPTWSLIKSPF
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]