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The map label for this gene is nifB [H]

Identifier: 39997892

GI number: 39997892

Start: 3078386

End: 3079258

Strand: Reverse

Name: nifB [H]

Synonym: GSU2799

Alternate gene names: 39997892

Gene position: 3079258-3078386 (Counterclockwise)

Preceding gene: 39997895

Following gene: 39997891

Centisome position: 80.73

GC content: 64.6

Gene sequence:

>873_bases
ATGGCAACGTCCTGTGACCAGTTGAAGAAGATACAGGGACACCCCTGTTTTGGCGGCAATCACCACAAGAACGGCCGGAT
GCACCTTGCCGTGGCGCCCCGCTGCAACATCAAGTGCGGCTACTGTTCCCGCAAGCATGACTGTGCCAACGAGTCACGCC
CCGGGGTGACGAGCCGGCTGCTCACCCCGGACGAGGCCATCGTCAAGGTGCGCGAGGTCATGGCGAGTCCGGTTGTGGGA
CCGATCATCAAGGTCATCGGCATCGCCGGCCCCGGCGATCCCCTGGCCAATGAGGAGACCTTCGAGACCTTCAGGCTCGT
GGGGGAAGAGTTCCCTCACCTCATCAAGTGCATGAGCACCAACGGCCTCCTGCTCCCCGAAAGCATCGACCGGCTCCACG
ACCTGGGGCTCCACAGCCTCACGGTGACCCTCAACGCCCTCGACCCAGCCGTCGGCAGCCGCATCTACGGCCACATCCTC
TATCACGGCACGGTGTACCGGGGGGAAGAGGGGGCGCGGATTCTCATCGAAAACCAGCTGGAGGGAATCCGGCGCGCCGT
CGGCCTGGGCATGACCGTCAAGGTCAACACCGTCCTCATTCCCGGGATCAATGACGGGCAGGTCAGCGACATAGGACGTA
CGGTGAAAGAGTTCGGCGCCTTTGTCATGAACATCATGCCCCTCATTCCCCAGGCGGAACTGGCCCATGTCGTGCCGCCG
ACGCCGAAAGAACTGGACGTGGTCCGCCGCGCCAACGAAGCGGTCATCGGCCAGTTCAAGCATTGCCGCCAGTGCCGGGC
GGATGCCGTGGGGCTCATCGGCGCCGAGTTGACCGTGGGCGAGTCGGCCTGCGCGCTGCCGCCCGCCCCCTGA

Upstream 100 bases:

>100_bases
CCGGCGGTCGAGAGACCCCGGGCTCGTTGCGTTTCACGGCAAGGGCGCTGGGGATCCAAGATCGGCGCCCTTTTCAGTTC
ATCATCCACGGAGGTCGACT

Downstream 100 bases:

>100_bases
GATCATGGAGATCGGTCCGTTCAACGGCGAATCCCTGGACCTGTTCCTGGCCCTGGCCCGTGCCGAAGGGTGGATCAGCG
ACCCCTGGGAGCTCACATTC

Product: radical SAM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCSRKHDCANESRPGVTSRLLTPDEAIVKVREVMASPVVG
PIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLIKCMSTNGLLLPESIDRLHDLGLHSLTVTLNALDPAVGSRIYGHIL
YHGTVYRGEEGARILIENQLEGIRRAVGLGMTVKVNTVLIPGINDGQVSDIGRTVKEFGAFVMNIMPLIPQAELAHVVPP
TPKELDVVRRANEAVIGQFKHCRQCRADAVGLIGAELTVGESACALPPAP

Sequences:

>Translated_290_residues
MATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCSRKHDCANESRPGVTSRLLTPDEAIVKVREVMASPVVG
PIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLIKCMSTNGLLLPESIDRLHDLGLHSLTVTLNALDPAVGSRIYGHIL
YHGTVYRGEEGARILIENQLEGIRRAVGLGMTVKVNTVLIPGINDGQVSDIGRTVKEFGAFVMNIMPLIPQAELAHVVPP
TPKELDVVRRANEAVIGQFKHCRQCRADAVGLIGAELTVGESACALPPAP
>Mature_289_residues
ATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCSRKHDCANESRPGVTSRLLTPDEAIVKVREVMASPVVGP
IIKVIGIAGPGDPLANEETFETFRLVGEEFPHLIKCMSTNGLLLPESIDRLHDLGLHSLTVTLNALDPAVGSRIYGHILY
HGTVYRGEEGARILIENQLEGIRRAVGLGMTVKVNTVLIPGINDGQVSDIGRTVKEFGAFVMNIMPLIPQAELAHVVPPT
PKELDVVRRANEAVIGQFKHCRQCRADAVGLIGAELTVGESACALPPAP

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31246; Mature: 31115

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCSRKHDCANESRPGVTSRL
CCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHCC
LTPDEAIVKVREVMASPVVGPIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLIKCMST
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NGLLLPESIDRLHDLGLHSLTVTLNALDPAVGSRIYGHILYHGTVYRGEEGARILIENQL
CCEECHHHHHHHHHCCCEEEEEEEEHHCHHHHHHHHEEEEEECEEEECCCCCEEEEHHHH
EGIRRAVGLGMTVKVNTVLIPGINDGQVSDIGRTVKEFGAFVMNIMPLIPQAELAHVVPP
HHHHHHHCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
TPKELDVVRRANEAVIGQFKHCRQCRADAVGLIGAELTVGESACALPPAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCCCC
>Mature Secondary Structure 
ATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCSRKHDCANESRPGVTSRL
CCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHCC
LTPDEAIVKVREVMASPVVGPIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLIKCMST
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NGLLLPESIDRLHDLGLHSLTVTLNALDPAVGSRIYGHILYHGTVYRGEEGARILIENQL
CCEECHHHHHHHHHCCCEEEEEEEEHHCHHHHHHHHEEEEEECEEEECCCCCEEEEHHHH
EGIRRAVGLGMTVKVNTVLIPGINDGQVSDIGRTVKEFGAFVMNIMPLIPQAELAHVVPP
HHHHHHHCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
TPKELDVVRRANEAVIGQFKHCRQCRADAVGLIGAELTVGESACALPPAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]