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The map label for this gene is glpK

Identifier: 39997855

GI number: 39997855

Start: 3043068

End: 3044558

Strand: Reverse

Name: glpK

Synonym: GSU2762

Alternate gene names: 39997855

Gene position: 3044558-3043068 (Counterclockwise)

Preceding gene: 39997856

Following gene: 39997854

Centisome position: 79.82

GC content: 71.43

Gene sequence:

>1491_bases
ATGAGCTTCATCCTCGCGCTTGACCAGGGGACGACCAGTTCCCGCGCCCTGGTATTCGACCATGACGGGAACGTGCTCGG
GCTCGCCCAGAAGGAGTTTCGCCAGATATTCCCCCAACCGGGCCTGGTTGAGCACGATGCCGAGGAGATCTGGGCCTCCC
AGCTGGGGGTGGTGGTGGAGGCCATTGCCCGGGCCGGGATCACCGCCGCGGACATTGCCGCCATCGGCATCACCAACCAG
CGGGAGACGACGGTGGTCTGGGATCGCCGCACCGGCCGGCCGATCCACCACGCCATCGTCTGGCAGGACCGGCGCACGGC
CGGCGAGTGCGACCGGCTCAGGGCCAAGGGGCTGGAGCCGACCTTCCGCAACAGGACCGGGCTGGTGCTGGACCCCTACT
TCTCGGGCACCAAGCTGGCCTGGCTGCTCGATAACGTGCCCGGGGCACGGGCCAGGGCCGAGGCGGGGGAACTGGCCTTC
GGTACGATCGATTCCTGGCTGGTCTGGAACCTGACCGGCGGCGAGCGGCACCTGACCGATGCCAGCAACGCCTCCCGGAC
GCTCCTGTTCAACATCCACGACGGCGCCTGGGACCCGGAACTGCTGGAGCTGCTCCGGATCCCGCCCGCGATCCTGCCCC
AGGTGGTGGCCTCCAGCGAGGTCTACGGCGAGACCGCCGCCCGCTTCCTGGCGGCGCGCATCCCCATCTGCGGCATCGCG
GGGGACCAGCAGGCGGCGCTGTTCGGTCAGCTCTGCGACCGGCCCGGGATGGTGAAGAACACGTACGGCACCGGCTGCTT
CATGCTGATGCAGACCGGCGAGCGGCCGGTGCTCTCGGGGCGGAACCTCCTGACCACCGTGGCCTGCCGACTGGGCAACC
GGACGGAGTACGCCCTGGAGGGGAGCGTGTTCGTGGCCGGCGCGGCGGTGCAGTGGCTGCGGGACGGGCTCGGCATCATC
CGCACCTCGGCGGAGGTGGAGACGCTGGCCGCCTCGGTCCCGGACAACGGCGGGGTCTACCTGGTGCCGGCCTTTGCGGG
GCTGGGCGCTCCCCATTGGGACCCCTACGCCCGGGGCGCCCTGCTGGGCATCACCCGCGGCACCACCGCCGGCCACATCG
CCCGGGCCACCTTGGAGAGCATCGCCTTCCAGACGGCCGATCTCCTGGAGGCCATGGAGGCCGATGCGGCTACCCCGCTG
GCCGAACTGCGGGTGGACGGCGGCGCCACGGCCAACAACATGCTGATGCAGTTCCAGGCCGACCTCCTGGGGGTGCCGGT
GGTGCGGCCCCGGGTGCGCGAGACCACGGCCCTGGGCGCTGCCTACCTGGCCGGGCTGGCGGTGGGCTACTGGCGGGACC
GGGCCGAGATCGGCCGGCTCTGGCAGGCGGAGCACACCTTTGAGCCGGCCATGGACCGGGACAGGGCGGCGGAGCTGCGC
TCCACCTGGAGCAGGGCCCTGGAAAGGTCCCAGGGGTGGGCGCAGCCATGA

Upstream 100 bases:

>100_bases
CGCCATGCTCGATGAGCTGCGCGGGCGGCTGGCGGACAGGGGTCAGCCCAACGCCTGAGACGGCGCCCCCGCCTCATTCA
TCCGGCAAAGGAGTCCTGCC

Downstream 100 bases:

>100_bases
AGCGCGAGGATCTGCTGCGGCATCTGGCCGAAACCGAGGTCTGGGACATGATCGTGATCGGCGGGGGCGCCACGGGCCTC
GGCACGGCGGTGGAGGCGGC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSFILALDQGTTSSRALVFDHDGNVLGLAQKEFRQIFPQPGLVEHDAEEIWASQLGVVVEAIARAGITAADIAAIGITNQ
RETTVVWDRRTGRPIHHAIVWQDRRTAGECDRLRAKGLEPTFRNRTGLVLDPYFSGTKLAWLLDNVPGARARAEAGELAF
GTIDSWLVWNLTGGERHLTDASNASRTLLFNIHDGAWDPELLELLRIPPAILPQVVASSEVYGETAARFLAARIPICGIA
GDQQAALFGQLCDRPGMVKNTYGTGCFMLMQTGERPVLSGRNLLTTVACRLGNRTEYALEGSVFVAGAAVQWLRDGLGII
RTSAEVETLAASVPDNGGVYLVPAFAGLGAPHWDPYARGALLGITRGTTAGHIARATLESIAFQTADLLEAMEADAATPL
AELRVDGGATANNMLMQFQADLLGVPVVRPRVRETTALGAAYLAGLAVGYWRDRAEIGRLWQAEHTFEPAMDRDRAAELR
STWSRALERSQGWAQP

Sequences:

>Translated_496_residues
MSFILALDQGTTSSRALVFDHDGNVLGLAQKEFRQIFPQPGLVEHDAEEIWASQLGVVVEAIARAGITAADIAAIGITNQ
RETTVVWDRRTGRPIHHAIVWQDRRTAGECDRLRAKGLEPTFRNRTGLVLDPYFSGTKLAWLLDNVPGARARAEAGELAF
GTIDSWLVWNLTGGERHLTDASNASRTLLFNIHDGAWDPELLELLRIPPAILPQVVASSEVYGETAARFLAARIPICGIA
GDQQAALFGQLCDRPGMVKNTYGTGCFMLMQTGERPVLSGRNLLTTVACRLGNRTEYALEGSVFVAGAAVQWLRDGLGII
RTSAEVETLAASVPDNGGVYLVPAFAGLGAPHWDPYARGALLGITRGTTAGHIARATLESIAFQTADLLEAMEADAATPL
AELRVDGGATANNMLMQFQADLLGVPVVRPRVRETTALGAAYLAGLAVGYWRDRAEIGRLWQAEHTFEPAMDRDRAAELR
STWSRALERSQGWAQP
>Mature_495_residues
SFILALDQGTTSSRALVFDHDGNVLGLAQKEFRQIFPQPGLVEHDAEEIWASQLGVVVEAIARAGITAADIAAIGITNQR
ETTVVWDRRTGRPIHHAIVWQDRRTAGECDRLRAKGLEPTFRNRTGLVLDPYFSGTKLAWLLDNVPGARARAEAGELAFG
TIDSWLVWNLTGGERHLTDASNASRTLLFNIHDGAWDPELLELLRIPPAILPQVVASSEVYGETAARFLAARIPICGIAG
DQQAALFGQLCDRPGMVKNTYGTGCFMLMQTGERPVLSGRNLLTTVACRLGNRTEYALEGSVFVAGAAVQWLRDGLGIIR
TSAEVETLAASVPDNGGVYLVPAFAGLGAPHWDPYARGALLGITRGTTAGHIARATLESIAFQTADLLEAMEADAATPLA
ELRVDGGATANNMLMQFQADLLGVPVVRPRVRETTALGAAYLAGLAVGYWRDRAEIGRLWQAEHTFEPAMDRDRAAELRS
TWSRALERSQGWAQP

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=48.00796812749, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=48.00796812749, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=47.1400394477318, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=506, Percent_Identity=46.6403162055336, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=33.2023575638507, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=58.5858585858586, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1790005, Length=471, Percent_Identity=25.6900212314225, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787789, Length=495, Percent_Identity=27.0707070707071, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789168, Length=471, Percent_Identity=24.416135881104, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789987, Length=495, Percent_Identity=23.4343434343434, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=510, Percent_Identity=42.156862745098, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=27.3076923076923, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=32.8358208955224, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=557, Percent_Identity=37.7019748653501, Blast_Score=319, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=43.9759036144578, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=43.9759036144578, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=43.9759036144578, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=43.9759036144578, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=43.9759036144578, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17864214, Length=507, Percent_Identity=43.9842209072978, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24654573, Length=447, Percent_Identity=45.8612975391499, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=33.6501901140684, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=33.9430894308943, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=31.9767441860465, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI21357867, Length=527, Percent_Identity=31.8785578747628, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=33.2378223495702, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI45552909, Length=285, Percent_Identity=31.2280701754386, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_GEOSL (Q749I1)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953806.1
- HSSP:   P0A6F3
- ProteinModelPortal:   Q749I1
- SMR:   Q749I1
- GeneID:   2686999
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2762
- NMPDR:   fig|243231.1.peg.2744
- TIGR:   GSU2762
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   CLSK743164
- BioCyc:   GSUL243231:GSU_2762-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53683; Mature: 53551

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFILALDQGTTSSRALVFDHDGNVLGLAQKEFRQIFPQPGLVEHDAEEIWASQLGVVVE
CCEEEEECCCCCCCEEEEEECCCCEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIARAGITAADIAAIGITNQRETTVVWDRRTGRPIHHAIVWQDRRTAGECDRLRAKGLEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHCCCCC
TFRNRTGLVLDPYFSGTKLAWLLDNVPGARARAEAGELAFGTIDSWLVWNLTGGERHLTD
CCCCCCCCEECCCCCCCEEEEEECCCCCCCHHCCCCCEEEECCCCEEEEEECCCCEECCC
ASNASRTLLFNIHDGAWDPELLELLRIPPAILPQVVASSEVYGETAARFLAARIPICGIA
CCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
GDQQAALFGQLCDRPGMVKNTYGTGCFMLMQTGERPVLSGRNLLTTVACRLGNRTEYALE
CCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEC
GSVFVAGAAVQWLRDGLGIIRTSAEVETLAASVPDNGGVYLVPAFAGLGAPHWDPYARGA
CCEEEEHHHHHHHHCCHHHEECCHHHHHHHHCCCCCCCEEEEEEHHCCCCCCCCHHHCCE
LLGITRGTTAGHIARATLESIAFQTADLLEAMEADAATPLAELRVDGGATANNMLMQFQA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHH
DLLGVPVVRPRVRETTALGAAYLAGLAVGYWRDRAEIGRLWQAEHTFEPAMDRDRAAELR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHH
STWSRALERSQGWAQP
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SFILALDQGTTSSRALVFDHDGNVLGLAQKEFRQIFPQPGLVEHDAEEIWASQLGVVVE
CEEEEECCCCCCCEEEEEECCCCEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIARAGITAADIAAIGITNQRETTVVWDRRTGRPIHHAIVWQDRRTAGECDRLRAKGLEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHCCCCC
TFRNRTGLVLDPYFSGTKLAWLLDNVPGARARAEAGELAFGTIDSWLVWNLTGGERHLTD
CCCCCCCCEECCCCCCCEEEEEECCCCCCCHHCCCCCEEEECCCCEEEEEECCCCEECCC
ASNASRTLLFNIHDGAWDPELLELLRIPPAILPQVVASSEVYGETAARFLAARIPICGIA
CCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
GDQQAALFGQLCDRPGMVKNTYGTGCFMLMQTGERPVLSGRNLLTTVACRLGNRTEYALE
CCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEC
GSVFVAGAAVQWLRDGLGIIRTSAEVETLAASVPDNGGVYLVPAFAGLGAPHWDPYARGA
CCEEEEHHHHHHHHCCHHHEECCHHHHHHHHCCCCCCCEEEEEEHHCCCCCCCCHHHCCE
LLGITRGTTAGHIARATLESIAFQTADLLEAMEADAATPLAELRVDGGATANNMLMQFQA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHH
DLLGVPVVRPRVRETTALGAAYLAGLAVGYWRDRAEIGRLWQAEHTFEPAMDRDRAAELR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHH
STWSRALERSQGWAQP
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA