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The map label for this gene is pleD [H]

Identifier: 39997726

GI number: 39997726

Start: 2902943

End: 2904178

Strand: Direct

Name: pleD [H]

Synonym: GSU2632

Alternate gene names: 39997726

Gene position: 2902943-2904178 (Clockwise)

Preceding gene: 39997725

Following gene: 39997727

Centisome position: 76.11

GC content: 65.37

Gene sequence:

>1236_bases
GTGAACACCCTGACGGCAAAACATTTCATCATCTTCGTGATTCCCGCTGCGCTTCTGGCGGCAACCTTCTGGTTCATGCC
CCGGGTGCCGGAGCTGCCCCCGGCACGACTTGAGCTACTCCCCCATCTCCCCTATCCGGCGATTGCCCTCGGCGCCATTC
TCTCCATCCACTTCCACCGGGGACGGGCATTGTTCGTCTTTTTACTGCTGGCTGTCTCGTACTGGAGCTTTCAGGGGAAT
GCGACGGAACTCCCCAGGGGAATCGAGGCCACGGTGCTCTACCAGGCCGTCACCCTGCTCGTGCCGCTCAACATCGCCCT
GTTCTCGCTCATGCGGGAACGCGGAATCGTTACGGTAGCGGGGCGAATCCGCTTCGCATTCCTGGGGCTCCAGGCACTGC
TCGTCTGGTGGGCCATGACACCGGGGCACGTGGCGGTCCAGCAGCTGCTGGCGGGTCATTTGAGCGCTGCCGGTGTGGTC
GCCGGGTCTCCTCTCCCACCTCCGGCCATCCCGGCAATAGTCCTCTCGGGCTTGCTGGTGGCGGCGCGGGCAGGACTGAA
ACAGTCTCCCGTGGACAGCGCGTTCCTGGGGGTCCTGGTGGCCTTTGCCGTGGCGGGCAACGGCATCGCCCACCCCCATG
TCACCCCGGTCTTCATGACTGCCGCGGCAGTTATCCTCTCACTCGGCGTGCTCAAGGATTCCTACAACATGGCGTTCCGG
GACGAGTTGACGGGTCTCCCGTCCCGCCGGGCGCTCAGCGAGCAGCTTACCTGGCTGGGCCGGCGCTACAGCGTTGCCAT
GGTTGACGTCGACCACTTCAAAAAGTTCAACGACACCTACGGACACGACGTGGGGGATCAGGTGCTCCGGCTGGTGGCGG
CAAAACTGCGCGGCGTGGGAGGTGGGGGCAAGGCCTACCGCTACGGCGGCGAAGAGTTCACCATCCTTTTCCCCCATCGG
TCGCGGGAAGAGGTCCTCGGCCGCCTGGAAGAGTTGCGACGGGCCATCGCCGATTACCAGATGCGCCTGCGCTCCTCGGA
CCGGCCCGCCTCAAGCAGGGAGGGAAAGAGGCAGCGCTCCGGCATGCCCCAAGGGCGCGGCTGCGTCTCGGTAACCATCA
GCATCGGCGTGGCTGAAAGCAGCAGCGAACTTCGCCGGCCCGCCGATGTCATCAAGGCGGCCGACGACGCCCTCTACCGC
GCCAAGGGGCGCGGCCGCAACCAGGTGAGCACCTGA

Upstream 100 bases:

>100_bases
GATAAAAATTATCGGCGAGTGACCCTGCGTCGGCCAAAAGATTGCATATTTCTCATCCTGTCCGATCCCTCTGACCACCT
GTCCAAGGAGCAGCGCCCGG

Downstream 100 bases:

>100_bases
CCCGGCAGAAGCCCCGGCTCTCCGCCTGCACCTGTCCTCCCCCGTGATTGCATTCTTCCGGCCGGCCGCTATACTTTTTC
CTTGTCACCCCACATCATCA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNTLTAKHFIIFVIPAALLAATFWFMPRVPELPPARLELLPHLPYPAIALGAILSIHFHRGRALFVFLLLAVSYWSFQGN
ATELPRGIEATVLYQAVTLLVPLNIALFSLMRERGIVTVAGRIRFAFLGLQALLVWWAMTPGHVAVQQLLAGHLSAAGVV
AGSPLPPPAIPAIVLSGLLVAARAGLKQSPVDSAFLGVLVAFAVAGNGIAHPHVTPVFMTAAAVILSLGVLKDSYNMAFR
DELTGLPSRRALSEQLTWLGRRYSVAMVDVDHFKKFNDTYGHDVGDQVLRLVAAKLRGVGGGGKAYRYGGEEFTILFPHR
SREEVLGRLEELRRAIADYQMRLRSSDRPASSREGKRQRSGMPQGRGCVSVTISIGVAESSSELRRPADVIKAADDALYR
AKGRGRNQVST

Sequences:

>Translated_411_residues
MNTLTAKHFIIFVIPAALLAATFWFMPRVPELPPARLELLPHLPYPAIALGAILSIHFHRGRALFVFLLLAVSYWSFQGN
ATELPRGIEATVLYQAVTLLVPLNIALFSLMRERGIVTVAGRIRFAFLGLQALLVWWAMTPGHVAVQQLLAGHLSAAGVV
AGSPLPPPAIPAIVLSGLLVAARAGLKQSPVDSAFLGVLVAFAVAGNGIAHPHVTPVFMTAAAVILSLGVLKDSYNMAFR
DELTGLPSRRALSEQLTWLGRRYSVAMVDVDHFKKFNDTYGHDVGDQVLRLVAAKLRGVGGGGKAYRYGGEEFTILFPHR
SREEVLGRLEELRRAIADYQMRLRSSDRPASSREGKRQRSGMPQGRGCVSVTISIGVAESSSELRRPADVIKAADDALYR
AKGRGRNQVST
>Mature_411_residues
MNTLTAKHFIIFVIPAALLAATFWFMPRVPELPPARLELLPHLPYPAIALGAILSIHFHRGRALFVFLLLAVSYWSFQGN
ATELPRGIEATVLYQAVTLLVPLNIALFSLMRERGIVTVAGRIRFAFLGLQALLVWWAMTPGHVAVQQLLAGHLSAAGVV
AGSPLPPPAIPAIVLSGLLVAARAGLKQSPVDSAFLGVLVAFAVAGNGIAHPHVTPVFMTAAAVILSLGVLKDSYNMAFR
DELTGLPSRRALSEQLTWLGRRYSVAMVDVDHFKKFNDTYGHDVGDQVLRLVAAKLRGVGGGGKAYRYGGEEFTILFPHR
SREEVLGRLEELRRAIADYQMRLRSSDRPASSREGKRQRSGMPQGRGCVSVTISIGVAESSSELRRPADVIKAADDALYR
AKGRGRNQVST

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=34.0540540540541, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787056, Length=198, Percent_Identity=32.8282828282828, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI145693134, Length=150, Percent_Identity=34, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787802, Length=214, Percent_Identity=31.3084112149533, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786584, Length=185, Percent_Identity=29.1891891891892, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787816, Length=202, Percent_Identity=32.1782178217822, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082007, Length=199, Percent_Identity=30.1507537688442, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=107, Percent_Identity=32.7102803738318, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=30.8988764044944, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44595; Mature: 44595

Theoretical pI: Translated: 10.80; Mature: 10.80

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLTAKHFIIFVIPAALLAATFWFMPRVPELPPARLELLPHLPYPAIALGAILSIHFHR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCC
GRALFVFLLLAVSYWSFQGNATELPRGIEATVLYQAVTLLVPLNIALFSLMRERGIVTVA
CHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEH
GRIRFAFLGLQALLVWWAMTPGHVAVQQLLAGHLSAAGVVAGSPLPPPAIPAIVLSGLLV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHH
AARAGLKQSPVDSAFLGVLVAFAVAGNGIAHPHVTPVFMTAAAVILSLGVLKDSYNMAFR
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
DELTGLPSRRALSEQLTWLGRRYSVAMVDVDHFKKFNDTYGHDVGDQVLRLVAAKLRGVG
HHHCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
GGGKAYRYGGEEFTILFPHRSREEVLGRLEELRRAIADYQMRLRSSDRPASSREGKRQRS
CCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHC
GMPQGRGCVSVTISIGVAESSSELRRPADVIKAADDALYRAKGRGRNQVST
CCCCCCCEEEEEEEEEECCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTLTAKHFIIFVIPAALLAATFWFMPRVPELPPARLELLPHLPYPAIALGAILSIHFHR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCC
GRALFVFLLLAVSYWSFQGNATELPRGIEATVLYQAVTLLVPLNIALFSLMRERGIVTVA
CHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEH
GRIRFAFLGLQALLVWWAMTPGHVAVQQLLAGHLSAAGVVAGSPLPPPAIPAIVLSGLLV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHH
AARAGLKQSPVDSAFLGVLVAFAVAGNGIAHPHVTPVFMTAAAVILSLGVLKDSYNMAFR
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
DELTGLPSRRALSEQLTWLGRRYSVAMVDVDHFKKFNDTYGHDVGDQVLRLVAAKLRGVG
HHHCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
GGGKAYRYGGEEFTILFPHRSREEVLGRLEELRRAIADYQMRLRSSDRPASSREGKRQRS
CCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHC
GMPQGRGCVSVTISIGVAESSSELRRPADVIKAADDALYRAKGRGRNQVST
CCCCCCCEEEEEEEEEECCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA