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The map label for this gene is cysD [H]

Identifier: 39996816

GI number: 39996816

Start: 1881178

End: 1881981

Strand: Direct

Name: cysD [H]

Synonym: GSU1717

Alternate gene names: 39996816

Gene position: 1881178-1881981 (Clockwise)

Preceding gene: 39996815

Following gene: 39996817

Centisome position: 49.32

GC content: 61.32

Gene sequence:

>804_bases
ATGAACAGCCATCTTGACGAACTCGAAGCGCAATCCATCTACATCTTTCGCGAGGCATACCGGAAGTTCGAGAATCTCGC
CATGCTCTATTCGGTGGGCAAAGACTCCACCACCATGATCCATTTGGCCCGCAAGGCCTTTTTCGGACGAATTCCCTTTC
CGCTGGTGCACATCGACACCACCTACAAATATCCGGAAATGATCGAATATCGGGACCGCATGGCACGGGAGTGGGGCGCG
GACCTGATCGTCGGGCGCAACGAATCGGCCATTGCCGCCGGCACGGGGCCCGAACAGGGGAGACTCGACTGCTGCGCCAA
GCTCAAGACCGACGGCCTGAGTCAGACCATCGAGCGTCACGGTTTCAACGGCCTCTTTCTCGGCATCCGCCGGGACGAGG
AGGGAAGCCGGGCCAAGGAACGGGTTTTTTCGCCCCGCGACAAGAACTTCGAGTGGTCCTACAAGGACCAGCCGCCCGAA
CTGTGGGACCAGTTCAACACGACCTTTGCGCCGGGCACCCACATCCGGGTCCACCCGATCCTGCACTGGACCGAACTGGA
CATCTGGCTCTACATCCAGCGGGAAGGGATCGAACTCTGCCCCCTGTACTTCGCCCGGGACGGCAAACGGTTCCGCTCCC
TCGGCTGCATGCCCTGCACCGGCCCCATCCAGTCGAACGCCGTCACGGTGGAGGAGATCATCGAGGAGCTCCGCGCCATC
AAGACACCGGAGCGGGCCGGCCGGGCCCAGGACCAGGAAAACACCTATGCCATGCAGAAGCTGCGGGCCAAGGGCTACAT
GTAA

Upstream 100 bases:

>100_bases
CCCCGGTCGGGGCGCTGGTGGTGGGAAGATCCTGAGCACAAGGAATGCGGGCTGCACCGGCGCTGACGCTAACAAGACAT
CCAATCGGAGAGAATGAACC

Downstream 100 bases:

>100_bases
GCGGGTAACCGGGTAAGGAGATACGATATGAGCCAGTCTGAAACACTGAAGATAGTTATTGTGGGGCATGTTGACCACGG
CAAGTCAACCCTGATCGGCC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNSHLDELEAQSIYIFREAYRKFENLAMLYSVGKDSTTMIHLARKAFFGRIPFPLVHIDTTYKYPEMIEYRDRMAREWGA
DLIVGRNESAIAAGTGPEQGRLDCCAKLKTDGLSQTIERHGFNGLFLGIRRDEEGSRAKERVFSPRDKNFEWSYKDQPPE
LWDQFNTTFAPGTHIRVHPILHWTELDIWLYIQREGIELCPLYFARDGKRFRSLGCMPCTGPIQSNAVTVEEIIEELRAI
KTPERAGRAQDQENTYAMQKLRAKGYM

Sequences:

>Translated_267_residues
MNSHLDELEAQSIYIFREAYRKFENLAMLYSVGKDSTTMIHLARKAFFGRIPFPLVHIDTTYKYPEMIEYRDRMAREWGA
DLIVGRNESAIAAGTGPEQGRLDCCAKLKTDGLSQTIERHGFNGLFLGIRRDEEGSRAKERVFSPRDKNFEWSYKDQPPE
LWDQFNTTFAPGTHIRVHPILHWTELDIWLYIQREGIELCPLYFARDGKRFRSLGCMPCTGPIQSNAVTVEEIIEELRAI
KTPERAGRAQDQENTYAMQKLRAKGYM
>Mature_267_residues
MNSHLDELEAQSIYIFREAYRKFENLAMLYSVGKDSTTMIHLARKAFFGRIPFPLVHIDTTYKYPEMIEYRDRMAREWGA
DLIVGRNESAIAAGTGPEQGRLDCCAKLKTDGLSQTIERHGFNGLFLGIRRDEEGSRAKERVFSPRDKNFEWSYKDQPPE
LWDQFNTTFAPGTHIRVHPILHWTELDIWLYIQREGIELCPLYFARDGKRFRSLGCMPCTGPIQSNAVTVEEIIEELRAI
KTPERAGRAQDQENTYAMQKLRAKGYM

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=295, Percent_Identity=44.7457627118644, Blast_Score=251, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 30965; Mature: 30965

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHLDELEAQSIYIFREAYRKFENLAMLYSVGKDSTTMIHLARKAFFGRIPFPLVHIDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
TYKYPEMIEYRDRMAREWGADLIVGRNESAIAAGTGPEQGRLDCCAKLKTDGLSQTIERH
CCCCHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHC
GFNGLFLGIRRDEEGSRAKERVFSPRDKNFEWSYKDQPPELWDQFNTTFAPGTHIRVHPI
CCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEE
LHWTELDIWLYIQREGIELCPLYFARDGKRFRSLGCMPCTGPIQSNAVTVEEIIEELRAI
EEEEEEEEEEEEEECCCEEEEHHHHCCCHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHH
KTPERAGRAQDQENTYAMQKLRAKGYM
CCCHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSHLDELEAQSIYIFREAYRKFENLAMLYSVGKDSTTMIHLARKAFFGRIPFPLVHIDT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
TYKYPEMIEYRDRMAREWGADLIVGRNESAIAAGTGPEQGRLDCCAKLKTDGLSQTIERH
CCCCHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHC
GFNGLFLGIRRDEEGSRAKERVFSPRDKNFEWSYKDQPPELWDQFNTTFAPGTHIRVHPI
CCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEE
LHWTELDIWLYIQREGIELCPLYFARDGKRFRSLGCMPCTGPIQSNAVTVEEIIEELRAI
EEEEEEEEEEEEEECCCEEEEHHHHCCCHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHH
KTPERAGRAQDQENTYAMQKLRAKGYM
CCCHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA