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The map label for this gene is dapF [H]

Identifier: 39995638

GI number: 39995638

Start: 564955

End: 565803

Strand: Reverse

Name: dapF [H]

Synonym: GSU0531

Alternate gene names: 39995638

Gene position: 565803-564955 (Counterclockwise)

Preceding gene: 39995639

Following gene: 39995637

Centisome position: 14.83

GC content: 62.43

Gene sequence:

>849_bases
ATGAAATTCACCAAAATGCATGGGGCCGGCAACGACTACGTCTATGTCAACTGCTTCGAGGAGACCGTAGGGAACCCTGA
GCAGACGGCCATCAAGGTTTCCAACCGCAACTTCGGCATCGGCTCCGACGGGCTCATCCTGATCATGCCCTCCGACAAGG
CCGACGTCCGGATGCGCATGTTCAACTCCGACGGATCCGAGTCCGAGATGTGCGGCAACGGCATCCGCTGCGTGGCCAAG
TACGCCTACGACCATGGCATCGTGACCAAGAAGGAGATCACCGCTGAAACCGGCGCGGGCGTCCTGACGCTCCAGCTCTT
CACCGGCGCCGACGGCAGGGTTGACCGGGTCCGCGTCAACATGGGCAAGCCGCGCCTCACCCGTGCGGAAATTCCCATGC
TGGGCGACCCGGGCGACGGCCAGGTGGTCGGTCAGCCTCTGAACATCCTCCACACCACGTTCAACATTACCTGCGTTTCC
ATGGGGAACCCCCACTGCGTCATCTTTGTGGATGACGTGGAGAACTTCCAGGTGGAAAAGTACGGCCCCCTCATCGAGAA
TCACGAGATGTTCCCCCGCCGAACCAACGTGGAATTCGTCCAGATCATCTCCCGCACCGAGGTACGCCAGAGGACCTGGG
AGCGGGGAGCCGGCGAAACGCTGGCTTGCGGCACCGGCGCCAGCGCCGTCTGCGTGGCCGGGGCTCTCAACGGCCTCACC
GAGAAGAATATCCTCAACCACCTATCCGGCGGCGACCTGGAGCTGGAGTGGGCCGAGGACGGCTTCCTCTACATGACCGG
TCCGGCCACCGAGGTGTTCAGCGGCGAAATCAATCTCGGCACGCTGTAG

Upstream 100 bases:

>100_bases
AGGGACGCGGGATTTTTTCTTGTTGGCGATCCCCGTTTCTGCTATTTCTAAAGGAATTTTAGCGATATTTCCACTTATAC
CAACGAGAGGGATGACTATC

Downstream 100 bases:

>100_bases
CAGGCGCTGCCGGCACAAACCAGGGGCCGGCGGCGGTCCGGTCGAACCGGGCCGTCGCCGGCCTTTCGTCTATCAGGCTG
AGGCAAACGCCATGGAGGAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKFTKMHGAGNDYVYVNCFEETVGNPEQTAIKVSNRNFGIGSDGLILIMPSDKADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVTKKEITAETGAGVLTLQLFTGADGRVDRVRVNMGKPRLTRAEIPMLGDPGDGQVVGQPLNILHTTFNITCVS
MGNPHCVIFVDDVENFQVEKYGPLIENHEMFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNGLT
EKNILNHLSGGDLELEWAEDGFLYMTGPATEVFSGEINLGTL

Sequences:

>Translated_282_residues
MKFTKMHGAGNDYVYVNCFEETVGNPEQTAIKVSNRNFGIGSDGLILIMPSDKADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVTKKEITAETGAGVLTLQLFTGADGRVDRVRVNMGKPRLTRAEIPMLGDPGDGQVVGQPLNILHTTFNITCVS
MGNPHCVIFVDDVENFQVEKYGPLIENHEMFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNGLT
EKNILNHLSGGDLELEWAEDGFLYMTGPATEVFSGEINLGTL
>Mature_282_residues
MKFTKMHGAGNDYVYVNCFEETVGNPEQTAIKVSNRNFGIGSDGLILIMPSDKADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVTKKEITAETGAGVLTLQLFTGADGRVDRVRVNMGKPRLTRAEIPMLGDPGDGQVVGQPLNILHTTFNITCVS
MGNPHCVIFVDDVENFQVEKYGPLIENHEMFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNGLT
EKNILNHLSGGDLELEWAEDGFLYMTGPATEVFSGEINLGTL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=39.3617021276596, Blast_Score=214, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30747; Mature: 30747

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKMHGAGNDYVYVNCFEETVGNPEQTAIKVSNRNFGIGSDGLILIMPSDKADVRMRM
CCEEEECCCCCCEEEEEEEHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
FNSDGSESEMCGNGIRCVAKYAYDHGIVTKKEITAETGAGVLTLQLFTGADGRVDRVRVN
ECCCCCCHHHCCCCCEEEEEEHHCCCCEEHHHCCCCCCCCEEEEEEEECCCCCEEEEEEE
MGKPRLTRAEIPMLGDPGDGQVVGQPLNILHTTFNITCVSMGNPHCVIFVDDVENFQVEK
CCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCCEEEEC
YGPLIENHEMFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNGLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCH
EKNILNHLSGGDLELEWAEDGFLYMTGPATEVFSGEINLGTL
HHHHHHHCCCCCEEEEECCCCEEEEECCCCEEEECCEEECCC
>Mature Secondary Structure
MKFTKMHGAGNDYVYVNCFEETVGNPEQTAIKVSNRNFGIGSDGLILIMPSDKADVRMRM
CCEEEECCCCCCEEEEEEEHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
FNSDGSESEMCGNGIRCVAKYAYDHGIVTKKEITAETGAGVLTLQLFTGADGRVDRVRVN
ECCCCCCHHHCCCCCEEEEEEHHCCCCEEHHHCCCCCCCCEEEEEEEECCCCCEEEEEEE
MGKPRLTRAEIPMLGDPGDGQVVGQPLNILHTTFNITCVSMGNPHCVIFVDDVENFQVEK
CCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCCEEEEC
YGPLIENHEMFPRRTNVEFVQIISRTEVRQRTWERGAGETLACGTGASAVCVAGALNGLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCH
EKNILNHLSGGDLELEWAEDGFLYMTGPATEVFSGEINLGTL
HHHHHHHCCCCCEEEEECCCCEEEEECCCCEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA