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The map label for this gene is nfo

Identifier: 39995636

GI number: 39995636

Start: 563536

End: 564426

Strand: Reverse

Name: nfo

Synonym: GSU0529

Alternate gene names: 39995636

Gene position: 564426-563536 (Counterclockwise)

Preceding gene: 39995637

Following gene: 39995635

Centisome position: 14.8

GC content: 64.42

Gene sequence:

>891_bases
ATGAACGACTATCTCGGCGCGCACATGTCCATCGCCGGCGGGATTCACAAGGCTCCGGCGCGGGGAAACCGGGTCGGGTG
CGGCGTGATCCAGGTCTTCACCCAGAACTCCAACCAGTGGCGGGGGAAGATGCCCACCGAGGGGGAGGCGGCTCTGTTCC
GTGAACAGTGGGAAGCCGCGGGCCTCCACGAAATCATCGCCCATGACATCTACCTCATCAACCTGGCGGCCCCGCCGGGC
GAGACACGGGACAAGAGCCTCGCGGCCTTCCGGGAGGAGATGGAGCGCTGTACCCGCCTCGGTATCGGCACTATCGTGAT
GCATCCCGGTGCCCACCTGGGCGACGGAGAAGAGACCGGCATCCGGCGGATCTGTGAGGCCTTCAACCGCCTGATCCCCG
CGGTTCCCGAGTTCACCGGCGTGATCCTGCTGGAGACCACCGCAGGCCAAGGCACCAGCCTGGGTCATACCTTCGAGCAG
TTGGCCGCCATTATCGCCGGGACCGCGTTTCCCGACCGCTTCGCGGTCTGCTTCGACACCTGCCACACCTTTGCCGCCGG
ATACGACTTCACCACCGGAGAGGGGTACCGGCGCGTCTTCGCCGAGTTCGACCGGCTGATCGGCCTGGACCGGCTCCGGT
GCTTCCACCTGAACGATTCCCGGAAGGGGCTCAACTCCCGGGTGGACCGGCACGAGCATATCGGCCGGGGAACGCTGGGG
CTGGAGCCGTTCCGGTTCCTCATGAACGATGACCGCTTCACGACGGTGCCCAAGATTCTCGAAACGCCAAAGGGAGATGA
CGACGAGTTTGATATCATGAACCTGAACACGCTCCGGAGACTCGTCCGCCGGCGCACAACAAAGGAAAGAACCGAGCCAT
GCCCGAGTTGA

Upstream 100 bases:

>100_bases
CCGCCCCATCTGGAGCGAACCCCATGCGGAAACCCTGCTCGCACCGGAGGAGTACGCCGGGCGGATCGCGCTGATCCGGG
ACAGGCTCGGGCTCTAGGCC

Downstream 100 bases:

>100_bases
TCGTAACCGACGCTGCCTGCACCCGTTGCGGCGCCTGCGTCTCCGTCTGCCCCTTGCGCATCGTCGAACTGCCCGAGGGG
AACCTGCCGCCCCGCTTCAC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNDYLGAHMSIAGGIHKAPARGNRVGCGVIQVFTQNSNQWRGKMPTEGEAALFREQWEAAGLHEIIAHDIYLINLAAPPG
ETRDKSLAAFREEMERCTRLGIGTIVMHPGAHLGDGEETGIRRICEAFNRLIPAVPEFTGVILLETTAGQGTSLGHTFEQ
LAAIIAGTAFPDRFAVCFDTCHTFAAGYDFTTGEGYRRVFAEFDRLIGLDRLRCFHLNDSRKGLNSRVDRHEHIGRGTLG
LEPFRFLMNDDRFTTVPKILETPKGDDDEFDIMNLNTLRRLVRRRTTKERTEPCPS

Sequences:

>Translated_296_residues
MNDYLGAHMSIAGGIHKAPARGNRVGCGVIQVFTQNSNQWRGKMPTEGEAALFREQWEAAGLHEIIAHDIYLINLAAPPG
ETRDKSLAAFREEMERCTRLGIGTIVMHPGAHLGDGEETGIRRICEAFNRLIPAVPEFTGVILLETTAGQGTSLGHTFEQ
LAAIIAGTAFPDRFAVCFDTCHTFAAGYDFTTGEGYRRVFAEFDRLIGLDRLRCFHLNDSRKGLNSRVDRHEHIGRGTLG
LEPFRFLMNDDRFTTVPKILETPKGDDDEFDIMNLNTLRRLVRRRTTKERTEPCPS
>Mature_296_residues
MNDYLGAHMSIAGGIHKAPARGNRVGCGVIQVFTQNSNQWRGKMPTEGEAALFREQWEAAGLHEIIAHDIYLINLAAPPG
ETRDKSLAAFREEMERCTRLGIGTIVMHPGAHLGDGEETGIRRICEAFNRLIPAVPEFTGVILLETTAGQGTSLGHTFEQ
LAAIIAGTAFPDRFAVCFDTCHTFAAGYDFTTGEGYRRVFAEFDRLIGLDRLRCFHLNDSRKGLNSRVDRHEHIGRGTLG
LEPFRFLMNDDRFTTVPKILETPKGDDDEFDIMNLNTLRRLVRRRTTKERTEPCPS

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=269, Percent_Identity=43.8661710037175, Blast_Score=214, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17531193, Length=282, Percent_Identity=36.8794326241135, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322735, Length=269, Percent_Identity=33.8289962825279, Blast_Score=178, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_GEOSL (Q74FS7)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951587.1
- ProteinModelPortal:   Q74FS7
- GeneID:   2686004
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0529
- NMPDR:   fig|243231.1.peg.525
- TIGR:   GSU0529
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   CLSK924410
- BioCyc:   GSUL243231:GSU_0529-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 33075; Mature: 33075

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4; PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYLGAHMSIAGGIHKAPARGNRVGCGVIQVFTQNSNQWRGKMPTEGEAALFREQWEAA
CCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHC
GLHEIIAHDIYLINLAAPPGETRDKSLAAFREEMERCTRLGIGTIVMHPGAHLGDGEETG
HHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
IRRICEAFNRLIPAVPEFTGVILLETTAGQGTSLGHTFEQLAAIIAGTAFPDRFAVCFDT
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
CHTFAAGYDFTTGEGYRRVFAEFDRLIGLDRLRCFHLNDSRKGLNSRVDRHEHIGRGTLG
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
LEPFRFLMNDDRFTTVPKILETPKGDDDEFDIMNLNTLRRLVRRRTTKERTEPCPS
CCHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNDYLGAHMSIAGGIHKAPARGNRVGCGVIQVFTQNSNQWRGKMPTEGEAALFREQWEAA
CCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHC
GLHEIIAHDIYLINLAAPPGETRDKSLAAFREEMERCTRLGIGTIVMHPGAHLGDGEETG
HHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
IRRICEAFNRLIPAVPEFTGVILLETTAGQGTSLGHTFEQLAAIIAGTAFPDRFAVCFDT
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
CHTFAAGYDFTTGEGYRRVFAEFDRLIGLDRLRCFHLNDSRKGLNSRVDRHEHIGRGTLG
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
LEPFRFLMNDDRFTTVPKILETPKGDDDEFDIMNLNTLRRLVRRRTTKERTEPCPS
CCHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA