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The map label for this gene is glmU

Identifier: 39995381

GI number: 39995381

Start: 278527

End: 279957

Strand: Reverse

Name: glmU

Synonym: GSU0271

Alternate gene names: 39995381

Gene position: 279957-278527 (Counterclockwise)

Preceding gene: 39995385

Following gene: 39995380

Centisome position: 7.34

GC content: 65.27

Gene sequence:

>1431_bases
ATGGATAATCTTGCGGCAATCATCCTTGCCGCCGGCAAGGGGACAAGGATGAAATCGGGGATCGTGAAAGTCATGCACCC
CCTGGCCGGCGCGCCGATGGTGGCATGGCCGGTGGCAGTGGCCCGACAGGCCGGCGCGGGACGCATCGTTGCCGTGGTCG
GCCACCAGGCCGAGCGTCTTCGCGAGCATTTCTCCAACGATGCCGACATCACCCTGGCCGTGCAGGAGGAGCAGCTCGGC
ACCGGGCACGCCGTTGCCTGCGCCGCCGGGGATCTGTCCGGCTTCAGCGGAAAAGTTCTCATTCTCTGCGGCGATGTCCC
CCTCATCCGCACCGAGACTCTGCGGGCCATGGTGACGGCCCACGAGGCGACCGGGGCGGTCCTCACCGTACTCACGGCCC
GTCAGGAAAATCCCCACGGTTATGGGCGCATCATCCGCGGCTTCGACGGCAGGGTCATCCGGATCGTGGAGGAAAAGGAT
GCGACCCCCGACGAGCGGAGCCGCACCGAGGTGAACGCCGGCATTTACTGCGCCGAGGCATCCTTTCTCTTCGATGCGGT
GAAGCGGATCGGCAACGATAACGCCCAGGGGGAATATTACCTTACCGATATCATCACCATGGCCAATGACCGGGGTCTGC
GCTGCACCGCCCATCCGGTGGCCGATCCGGTGGAGGTCATGGGGATCAACGACCGGGTCCAGCTGGCCGAGGCAGCCCGG
CACGCCCGCCGTCGAATCGCCGAGGAGCATATGCTGAACGGCGTCACCCTGGTTGACCCGGCGGCCACCTACATCGACCA
GGGGGTCGTCATCGGCGCCGACACCACGATTCAGCCGGGAGTCCAGATCGCAGGCGGCTGCCGGGTCGGCGAGGGGTGCA
CCATCGAGGCCGGCGCAATCATCAAGGGGAGCGAACTGGGCGACCGCTGCGTGGTGGAATCGCGGGCGGTGATCCGGGGC
TGCCGGCTCGGCAGCGACGTGGTCATCAAAGCGGGTACCGTCATGGAGGATTCCACGGTCATGGACCACGCCGCCATCGG
CCCCATGGCCCACCTGCGCCCCGGCAGCGAGCTCGGGGCCCACGTGAAGATCGGCAACTTCGTGGAAACCAAGAAGATCG
TCATGGGAGAGGGGTCCAAGGCATCCCACCTGACCTACCTGGGGGACGCCACCATCGGCCGGAACGTGAACGTGGGCTGC
GGCACCATTACCTGTAACTATGACGGCGTGAACAAGCATCGGACCGTCATCGGTGACGACGTCTTCGTCGGGAGCGACGT
GCAGTTCGTGGCGCCGGTCACCATCGGCAGCAACACCCTCATCGCGGCAGGCACCACAGTGACCCGGGATGTACCAGCGG
ATTCGCTGGCCATAGCCCGGACGCCGCAGATAAACAAGGAAGGCTGGAAACTCAGGAAACGGGATCAGTAA

Upstream 100 bases:

>100_bases
TTCGCCACGTTAATCACTTGACTGGAAACCTCGGCATCGTTTAACGTTACTTGTTTTCGAATACGAATTATTTCCGACGC
TTTCAGTCCGGGAGGAACCC

Downstream 100 bases:

>100_bases
TCATGACGCGGGGATCGCTGACACGCGCCCCCACTGAGCCCCCCATACAAACGGAGACCTGATCTATGTGCGGAATAGTC
GGCTATATCGGCGCTCAGGA

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MDNLAAIILAAGKGTRMKSGIVKVMHPLAGAPMVAWPVAVARQAGAGRIVAVVGHQAERLREHFSNDADITLAVQEEQLG
TGHAVACAAGDLSGFSGKVLILCGDVPLIRTETLRAMVTAHEATGAVLTVLTARQENPHGYGRIIRGFDGRVIRIVEEKD
ATPDERSRTEVNAGIYCAEASFLFDAVKRIGNDNAQGEYYLTDIITMANDRGLRCTAHPVADPVEVMGINDRVQLAEAAR
HARRRIAEEHMLNGVTLVDPAATYIDQGVVIGADTTIQPGVQIAGGCRVGEGCTIEAGAIIKGSELGDRCVVESRAVIRG
CRLGSDVVIKAGTVMEDSTVMDHAAIGPMAHLRPGSELGAHVKIGNFVETKKIVMGEGSKASHLTYLGDATIGRNVNVGC
GTITCNYDGVNKHRTVIGDDVFVGSDVQFVAPVTIGSNTLIAAGTTVTRDVPADSLAIARTPQINKEGWKLRKRDQ

Sequences:

>Translated_476_residues
MDNLAAIILAAGKGTRMKSGIVKVMHPLAGAPMVAWPVAVARQAGAGRIVAVVGHQAERLREHFSNDADITLAVQEEQLG
TGHAVACAAGDLSGFSGKVLILCGDVPLIRTETLRAMVTAHEATGAVLTVLTARQENPHGYGRIIRGFDGRVIRIVEEKD
ATPDERSRTEVNAGIYCAEASFLFDAVKRIGNDNAQGEYYLTDIITMANDRGLRCTAHPVADPVEVMGINDRVQLAEAAR
HARRRIAEEHMLNGVTLVDPAATYIDQGVVIGADTTIQPGVQIAGGCRVGEGCTIEAGAIIKGSELGDRCVVESRAVIRG
CRLGSDVVIKAGTVMEDSTVMDHAAIGPMAHLRPGSELGAHVKIGNFVETKKIVMGEGSKASHLTYLGDATIGRNVNVGC
GTITCNYDGVNKHRTVIGDDVFVGSDVQFVAPVTIGSNTLIAAGTTVTRDVPADSLAIARTPQINKEGWKLRKRDQ
>Mature_476_residues
MDNLAAIILAAGKGTRMKSGIVKVMHPLAGAPMVAWPVAVARQAGAGRIVAVVGHQAERLREHFSNDADITLAVQEEQLG
TGHAVACAAGDLSGFSGKVLILCGDVPLIRTETLRAMVTAHEATGAVLTVLTARQENPHGYGRIIRGFDGRVIRIVEEKD
ATPDERSRTEVNAGIYCAEASFLFDAVKRIGNDNAQGEYYLTDIITMANDRGLRCTAHPVADPVEVMGINDRVQLAEAAR
HARRRIAEEHMLNGVTLVDPAATYIDQGVVIGADTTIQPGVQIAGGCRVGEGCTIEAGAIIKGSELGDRCVVESRAVIRG
CRLGSDVVIKAGTVMEDSTVMDHAAIGPMAHLRPGSELGAHVKIGNFVETKKIVMGEGSKASHLTYLGDATIGRNVNVGC
GTITCNYDGVNKHRTVIGDDVFVGSDVQFVAPVTIGSNTLIAAGTTVTRDVPADSLAIARTPQINKEGWKLRKRDQ

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=470, Percent_Identity=46.1702127659574, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_GEOSL (Q74GH5)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951332.1
- ProteinModelPortal:   Q74GH5
- SMR:   Q74GH5
- GeneID:   2687506
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0271
- NMPDR:   fig|243231.1.peg.270
- TIGR:   GSU0271
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK14355
- BioCyc:   GSUL243231:GSU_0271-MONOMER
- BRENDA:   2.3.1.157
- BRENDA:   2.7.7.23
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 50315; Mature: 50315

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 383-383 BINDING 75-75 BINDING 142-142 BINDING 157-157 BINDING 172-172 BINDING 407-407 BINDING 425-425 BINDING 443-443 BINDING 460-460

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLAAIILAAGKGTRMKSGIVKVMHPLAGAPMVAWPVAVARQAGAGRIVAVVGHQAERL
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHH
REHFSNDADITLAVQEEQLGTGHAVACAAGDLSGFSGKVLILCGDVPLIRTETLRAMVTA
HHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHEEH
HEATGAVLTVLTARQENPHGYGRIIRGFDGRVIRIVEEKDATPDERSRTEVNAGIYCAEA
HCCCCEEEEEEEECCCCCCCCHHEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEHH
SFLFDAVKRIGNDNAQGEYYLTDIITMANDRGLRCTAHPVADPVEVMGINDRVQLAEAAR
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCCEEEEECCCHHHHHHHHH
HARRRIAEEHMLNGVTLVDPAATYIDQGVVIGADTTIQPGVQIAGGCRVGEGCTIEAGAI
HHHHHHHHHHHHCCEEEECCHHHHHCCCEEEECCCCCCCCCEEECCEECCCCCEEECCCE
IKGSELGDRCVVESRAVIRGCRLGSDVVIKAGTVMEDSTVMDHAAIGPMAHLRPGSELGA
EECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCEECCCHHHHHHHCCCHHHCCCCCCCCC
HVKIGNFVETKKIVMGEGSKASHLTYLGDATIGRNVNVGCGTITCNYDGVNKHRTVIGDD
EEEECCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCC
VFVGSDVQFVAPVTIGSNTLIAAGTTVTRDVPADSLAIARTPQINKEGWKLRKRDQ
EEECCCCEEEEEEEECCCEEEEECCEEECCCCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDNLAAIILAAGKGTRMKSGIVKVMHPLAGAPMVAWPVAVARQAGAGRIVAVVGHQAERL
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHH
REHFSNDADITLAVQEEQLGTGHAVACAAGDLSGFSGKVLILCGDVPLIRTETLRAMVTA
HHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHEEH
HEATGAVLTVLTARQENPHGYGRIIRGFDGRVIRIVEEKDATPDERSRTEVNAGIYCAEA
HCCCCEEEEEEEECCCCCCCCHHEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEHH
SFLFDAVKRIGNDNAQGEYYLTDIITMANDRGLRCTAHPVADPVEVMGINDRVQLAEAAR
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCCEEEEECCCHHHHHHHHH
HARRRIAEEHMLNGVTLVDPAATYIDQGVVIGADTTIQPGVQIAGGCRVGEGCTIEAGAI
HHHHHHHHHHHHCCEEEECCHHHHHCCCEEEECCCCCCCCCEEECCEECCCCCEEECCCE
IKGSELGDRCVVESRAVIRGCRLGSDVVIKAGTVMEDSTVMDHAAIGPMAHLRPGSELGA
EECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCEECCCHHHHHHHCCCHHHCCCCCCCCC
HVKIGNFVETKKIVMGEGSKASHLTYLGDATIGRNVNVGCGTITCNYDGVNKHRTVIGDD
EEEECCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCC
VFVGSDVQFVAPVTIGSNTLIAAGTTVTRDVPADSLAIARTPQINKEGWKLRKRDQ
EEECCCCEEEEEEEECCCEEEEECCEEECCCCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA