Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 38234186

GI number: 38234186

Start: 1642793

End: 1643776

Strand: Reverse

Name: metF [H]

Synonym: DIP1611

Alternate gene names: 38234186

Gene position: 1643776-1642793 (Counterclockwise)

Preceding gene: 38234189

Following gene: 38234184

Centisome position: 66.05

GC content: 52.74

Gene sequence:

>984_bases
ATGTCTGCACAACCGCTACCTGCTGCGTATCAGCGCACAATCACCGATGTCATTTCCATGCCAACACCGGGCCAGGTTCC
GTTTTCTGTAGAGTTTATGCCGCCACGAGATGAGGCAGCAGAAGAGCGACTCTGGAAAGCCGCCGAAGCATTTCACGACT
TAGGAGCCTCTTTTGTCTCCGTTACTTATGGTGCAGGCGGATCTAGCCGCGAGCGCACAATGCGTGTCGCGCACAAGCTT
TCTCGTCATCCGTTGACCACGCTCGTTCATCTCACGCTTGTGGAACACACCCAAGAAGAATTAGAAGAAATTCTGTGCAC
TTATGCGTCCCACGGGTTGTCTAACTTACTTGCCTTGCGAGGCGATCCCCCTGGCACTGACCCGATGGCTCCGTGGGTCC
CTACCGCAGGCGGCCTAGATTATGCCAAAGATTTGATCGACCTCGTGCGCAAGACTGAGCAGACCTCGCACTTTCAGGTA
GGAATTGCTAGTTTCCCAGAAGGGCACTACCGAGCGCCTAGCATTGAGGCGGATACGCAATTTACATTGGAAAAGCTGCG
AGCTGGCGCAGAGTTTTCGATTACCCAGATGTTTTTTGATGTCGATCACTATTTACGACTGCGAGATCGCTTGGTTAAGG
CGGATCCTGAACATGGATCAAAGCCGATCATCCCAGGACTTATGCCCATTACCAGCTTGAGGTCGGTTCGTAGGCAGATG
GAATTAGCAGGTGCCACCTTGCCTAAGGCTTTAGAAAAACGGCTTCTCGACGCAGCGCGCGGCGATGAGGAAGCTCATCG
CGGCGATATTCGCAAAGTAGGAATCGAAGTCACTACTGAGATGGCACAGCGTCTTATTTCTGAAGGGATCCCAGACATCC
ATTTCATGACCATGAATTATGTTCGAGCGACCCAAGAAGTACTCCATAATCTCGGCATGGCGCCCGCGTGGGGAACACAG
CAAGGCCACGACGCTATTCGCTAA

Upstream 100 bases:

>100_bases
AGTGGTATCGACGCTGTTCAATAACGGCAACCTACAGTAAAAATGAATAAAATTCCTCAAGGTGGCAATATTCTTCAATT
TTCCCATAAAATACGCCCGT

Downstream 100 bases:

>100_bases
TTGCGATGGGCTTTCCGCTGTAGAACGGCGAAGGGGCGACGATCTCCTTCAAACATAAAATTCCGAAGGAGGTCATCAAA
ACCACAAGAGCGGTAAAGGT

Product: putative methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSAQPLPAAYQRTITDVISMPTPGQVPFSVEFMPPRDEAAEERLWKAAEAFHDLGASFVSVTYGAGGSSRERTMRVAHKL
SRHPLTTLVHLTLVEHTQEELEEILCTYASHGLSNLLALRGDPPGTDPMAPWVPTAGGLDYAKDLIDLVRKTEQTSHFQV
GIASFPEGHYRAPSIEADTQFTLEKLRAGAEFSITQMFFDVDHYLRLRDRLVKADPEHGSKPIIPGLMPITSLRSVRRQM
ELAGATLPKALEKRLLDAARGDEEAHRGDIRKVGIEVTTEMAQRLISEGIPDIHFMTMNYVRATQEVLHNLGMAPAWGTQ
QGHDAIR

Sequences:

>Translated_327_residues
MSAQPLPAAYQRTITDVISMPTPGQVPFSVEFMPPRDEAAEERLWKAAEAFHDLGASFVSVTYGAGGSSRERTMRVAHKL
SRHPLTTLVHLTLVEHTQEELEEILCTYASHGLSNLLALRGDPPGTDPMAPWVPTAGGLDYAKDLIDLVRKTEQTSHFQV
GIASFPEGHYRAPSIEADTQFTLEKLRAGAEFSITQMFFDVDHYLRLRDRLVKADPEHGSKPIIPGLMPITSLRSVRRQM
ELAGATLPKALEKRLLDAARGDEEAHRGDIRKVGIEVTTEMAQRLISEGIPDIHFMTMNYVRATQEVLHNLGMAPAWGTQ
QGHDAIR
>Mature_326_residues
SAQPLPAAYQRTITDVISMPTPGQVPFSVEFMPPRDEAAEERLWKAAEAFHDLGASFVSVTYGAGGSSRERTMRVAHKLS
RHPLTTLVHLTLVEHTQEELEEILCTYASHGLSNLLALRGDPPGTDPMAPWVPTAGGLDYAKDLIDLVRKTEQTSHFQVG
IASFPEGHYRAPSIEADTQFTLEKLRAGAEFSITQMFFDVDHYLRLRDRLVKADPEHGSKPIIPGLMPITSLRSVRRQME
LAGATLPKALEKRLLDAARGDEEAHRGDIRKVGIEVTTEMAQRLISEGIPDIHFMTMNYVRATQEVLHNLGMAPAWGTQQ
GHDAIR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=297, Percent_Identity=34.6801346801347, Blast_Score=157, Evalue=9e-39,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=34.3537414965986, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25147436, Length=300, Percent_Identity=31.6666666666667, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25147441, Length=300, Percent_Identity=31.6666666666667, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=302, Percent_Identity=32.4503311258278, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6325234, Length=301, Percent_Identity=31.5614617940199, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 36278; Mature: 36147

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQPLPAAYQRTITDVISMPTPGQVPFSVEFMPPRDEAAEERLWKAAEAFHDLGASFVS
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHHE
VTYGAGGSSRERTMRVAHKLSRHPLTTLVHLTLVEHTQEELEEILCTYASHGLSNLLALR
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDPPGTDPMAPWVPTAGGLDYAKDLIDLVRKTEQTSHFQVGIASFPEGHYRAPSIEADTQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHH
FTLEKLRAGAEFSITQMFFDVDHYLRLRDRLVKADPEHGSKPIIPGLMPITSLRSVRRQM
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ELAGATLPKALEKRLLDAARGDEEAHRGDIRKVGIEVTTEMAQRLISEGIPDIHFMTMNY
HHHCCHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
VRATQEVLHNLGMAPAWGTQQGHDAIR
HHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAQPLPAAYQRTITDVISMPTPGQVPFSVEFMPPRDEAAEERLWKAAEAFHDLGASFVS
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHHE
VTYGAGGSSRERTMRVAHKLSRHPLTTLVHLTLVEHTQEELEEILCTYASHGLSNLLALR
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDPPGTDPMAPWVPTAGGLDYAKDLIDLVRKTEQTSHFQVGIASFPEGHYRAPSIEADTQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHH
FTLEKLRAGAEFSITQMFFDVDHYLRLRDRLVKADPEHGSKPIIPGLMPITSLRSVRRQM
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
ELAGATLPKALEKRLLDAARGDEEAHRGDIRKVGIEVTTEMAQRLISEGIPDIHFMTMNY
HHHCCHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
VRATQEVLHNLGMAPAWGTQQGHDAIR
HHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515933 [H]