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The map label for this gene is miaA

Identifier: 38234024

GI number: 38234024

Start: 1462348

End: 1463256

Strand: Reverse

Name: miaA

Synonym: DIP1443

Alternate gene names: 38234024

Gene position: 1463256-1462348 (Counterclockwise)

Preceding gene: 38234025

Following gene: 38234023

Centisome position: 58.8

GC content: 48.4

Gene sequence:

>909_bases
ATGGATAATCCCACGACACCTATTGCGGTTGTTGGGCCGACTGCGTCAGGTAAGTCTGCGCTCGGAGTATCGTTAGCGCA
TCATCTTAACGGCGAGGTAGTCAACGTCGATTCGATGCAGCTTTATAAAGGCATGGATATCGGCACCGCCAAGCTCACAC
TTGAGGAACGCGAAGGCATTGTGCATCATTTGCTTGATGTGTGGGATATCTCTGAAACAGCGTCGGTTGCACGTTATCAA
AGCCAAGCGATTGCTGTGGTAGAAGATATTCAACGCCGTGGCAAAACCCCCATTTTAGTTGGTGGATCGATGCTTTATGT
ACAATCGCTTGTCGACGATTGGCAATTCCCGCCCACTGATCCATCCGTGCGTGCGAAATGGGAATCAAAACTTAATGAAG
TTGGCGTTGAAGCGCTTCACGCACTATTGGCGCAAAAAGATCCTCAAGCAGCTAGCATCATTGAACTTAAGGATCCTCGT
CGAACTGTACGGGCGTTAGAAGTTATTGAGTTGACTGGAAAGCCCTTCAACGCCAGTCAGCCCCCTAAGAATGCTCCACC
ACGCTGGAATACCCAGATTATTGGGTTAGGTACTAATACAGAGTGGCTTAACCCTCGAATCGATCTTCGCACAGAGCTGA
TGTTTGAAAAAGGCTTTGTCCACGAGGTAGAAAACCTCGTAGGAAAAGGGCTGATAGCTGATTCGACAGCTGGTCATGCC
ATTGGGTATGCGCAAGTACTTCAACATTTTGCTGGTGAGCTGAGTGTTGATGAGATGATTGAGCACACTAAGATCGGAAC
TCGTCGCTACGTGCGTAGGCAACGAAGCTGGTTCAAACGCGATCCAAGAATTCATTGGATCGATGCGAGCGGAGATACTT
TCTCTATGGCGTTATCATTGCTGTCATGA

Upstream 100 bases:

>100_bases
TGGGCAGATACGTGGCGTTATTGCAACCATGCTCTTGAATCGAATAATGCACTGCGTCAATTTCTGGGGAAACAAAATCA
AAGAAAGGCAATCCGATCGT

Downstream 100 bases:

>100_bases
CCTACATGAATACTCACGATGCACAGTTGCCCGTCTATAAAGGGCACGGCACGGAAAATGATTTCGTTATCATCCCGGAC
TTAGATGCGAAAATTGAGCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDNPTTPIAVVGPTASGKSALGVSLAHHLNGEVVNVDSMQLYKGMDIGTAKLTLEEREGIVHHLLDVWDISETASVARYQ
SQAIAVVEDIQRRGKTPILVGGSMLYVQSLVDDWQFPPTDPSVRAKWESKLNEVGVEALHALLAQKDPQAASIIELKDPR
RTVRALEVIELTGKPFNASQPPKNAPPRWNTQIIGLGTNTEWLNPRIDLRTELMFEKGFVHEVENLVGKGLIADSTAGHA
IGYAQVLQHFAGELSVDEMIEHTKIGTRRYVRRQRSWFKRDPRIHWIDASGDTFSMALSLLS

Sequences:

>Translated_302_residues
MDNPTTPIAVVGPTASGKSALGVSLAHHLNGEVVNVDSMQLYKGMDIGTAKLTLEEREGIVHHLLDVWDISETASVARYQ
SQAIAVVEDIQRRGKTPILVGGSMLYVQSLVDDWQFPPTDPSVRAKWESKLNEVGVEALHALLAQKDPQAASIIELKDPR
RTVRALEVIELTGKPFNASQPPKNAPPRWNTQIIGLGTNTEWLNPRIDLRTELMFEKGFVHEVENLVGKGLIADSTAGHA
IGYAQVLQHFAGELSVDEMIEHTKIGTRRYVRRQRSWFKRDPRIHWIDASGDTFSMALSLLS
>Mature_302_residues
MDNPTTPIAVVGPTASGKSALGVSLAHHLNGEVVNVDSMQLYKGMDIGTAKLTLEEREGIVHHLLDVWDISETASVARYQ
SQAIAVVEDIQRRGKTPILVGGSMLYVQSLVDDWQFPPTDPSVRAKWESKLNEVGVEALHALLAQKDPQAASIIELKDPR
RTVRALEVIELTGKPFNASQPPKNAPPRWNTQIIGLGTNTEWLNPRIDLRTELMFEKGFVHEVENLVGKGLIADSTAGHA
IGYAQVLQHFAGELSVDEMIEHTKIGTRRYVRRQRSWFKRDPRIHWIDASGDTFSMALSLLS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=28.6173633440514, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1790613, Length=285, Percent_Identity=39.6491228070175, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=241, Percent_Identity=30.7053941908714, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=310, Percent_Identity=32.258064516129, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24649830, Length=276, Percent_Identity=30.7971014492754, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CORDI (Q6NGR5)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939791.1
- ProteinModelPortal:   Q6NGR5
- SMR:   Q6NGR5
- GeneID:   2649714
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1443
- NMPDR:   fig|257309.1.peg.1382
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q6NGR5
- ProtClustDB:   PRK00091
- BioCyc:   CDIP257309:DIP1443-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33467; Mature: 33467

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNPTTPIAVVGPTASGKSALGVSLAHHLNGEVVNVDSMQLYKGMDIGTAKLTLEEREGI
CCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEEHHHHHH
VHHLLDVWDISETASVARYQSQAIAVVEDIQRRGKTPILVGGSMLYVQSLVDDWQFPPTD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCC
PSVRAKWESKLNEVGVEALHALLAQKDPQAASIIELKDPRRTVRALEVIELTGKPFNASQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCC
PPKNAPPRWNTQIIGLGTNTEWLNPRIDLRTELMFEKGFVHEVENLVGKGLIADSTAGHA
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCH
IGYAQVLQHFAGELSVDEMIEHTKIGTRRYVRRQRSWFKRDPRIHWIDASGDTFSMALSL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
LS
CC
>Mature Secondary Structure
MDNPTTPIAVVGPTASGKSALGVSLAHHLNGEVVNVDSMQLYKGMDIGTAKLTLEEREGI
CCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEECCHHHHHCCCCCCEEEEEEHHHHHH
VHHLLDVWDISETASVARYQSQAIAVVEDIQRRGKTPILVGGSMLYVQSLVDDWQFPPTD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCC
PSVRAKWESKLNEVGVEALHALLAQKDPQAASIIELKDPRRTVRALEVIELTGKPFNASQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCC
PPKNAPPRWNTQIIGLGTNTEWLNPRIDLRTELMFEKGFVHEVENLVGKGLIADSTAGHA
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCH
IGYAQVLQHFAGELSVDEMIEHTKIGTRRYVRRQRSWFKRDPRIHWIDASGDTFSMALSL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
LS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910