Click here to switch to the map view.

The map label for this gene is ptsI [H]

Identifier: 38234013

GI number: 38234013

Start: 1448538

End: 1450232

Strand: Reverse

Name: ptsI [H]

Synonym: DIP1428

Alternate gene names: 38234013

Gene position: 1450232-1448538 (Counterclockwise)

Preceding gene: 38234019

Following gene: 38234010

Centisome position: 58.27

GC content: 52.51

Gene sequence:

>1695_bases
TTGAATAACGTGATGACAGACACAATTATTAAGGGTACTGGCGTAGTTTCCGGTGTCCGTTATGCCAAAGCCGTGTGGAT
TAGCCCGCGGCCAAAGCTGCCCCAGGCAGGAGAGGTTGTTGACGAAGCTGATCGTGCTCAACAGCTAGATAACTTCGTTG
CAGCGGCAGATGCTGTGGCACAACGCCTTCTTGATCGTTCCGAACATGCCGAGGGTGCAGCCTCGGAGGTGCTAAAAGCT
ACTGCAGGAATGGTCAAAGACCGCGGCTGGCAGAAGGCCGTCAAGAAAGGCATTCAAGGCGGACACCCAGCTGAGTATGC
AGTTGTTGCTGCGACCACCAAGTTTGTTTCAATGTTTGAGGCAGCTGGCGGTGTAATGGCGGAGCGTACAACAGACCTTC
GCGATATTCGCGATCGCGTGATTGCTGAGCTACGAGGAGATCTTGAACCTGGACTTCCAGATGTTCATGAGCAGGTTGTG
CTTTTCGCTGATGACCTTTCCCCCGCAGATACCGCAGCCTTGAATACCGACTATTTTGTCGGTCTTGTAACTGAGTTGGG
TGGTCCAACCAGCCACACCGCGATTATTGCACGCCAGCTCAACGTTCCTTGCATTGTCGCAACCGGTGAGAAAATTCACA
AGATTGAAGTTGGTACAGACGTGCTTATCGACGGTGCATTGGGCACCGTGACTCTCGACGCAGATCCTGAAACCGCTCGT
GCAGCTGAACTGGAATCGCGCATGCTAGCTGAGCGCATCGCGCAGTGGAAAGGACCAGCCGAAACTAAGGACGGTCATCG
AGTGCAGCTACTTGCGAACGTACAAGATGGAAAAGCTGCACGGATCGCTGCAACGGAGAGTCAGGCTGAAGGTATTGGGT
TATTCCGTACTGAAATGTGCTTCTTGACCGCTACAGAAGAACCAAGCGTTGATGAACAAGCGGCTGTCTACAAAAAAGTT
TTGGAACAATTCCCAGATTCCAAGGTTGTTGTTCGTTCGCTAGATGCCGGTTCTGACAAACCAGTTGCCTTTGCGTCTAT
GGCTGACGAGATGAATCCTGCTCTGGGCGTTCGCGGCCTTCGCGTAGCCCGTGCTAATGAGTCATTGTTGACTCGCCAGT
TGGACGCTATCGCACAGGCCGCTCATGAGCTTGGTCGAGATGAGAATTCCCCAACTTGGGTTATGGCACCTATGGTGGCC
ACAACACGAGAGGCACGCTGGTTCGCAGGGCTTTGCGCAGAACGAGGATTAACCGCTGGTGCAATGATTGAGGTCCCTGC
AGCGTCGCTAATGGCAGATAAGCTCATGCCATATCTGGATTTCGTCTCTATTGGTACCAATGATCTCACCCAGTACACCA
TGGCAGCAGACAGAATGTCTCCTCAGCTTGCATATCTGACAGATCCATGGCAGCCCGCAGTGTTGCGTTTGATTAAGCAC
ACTTGTGAAGAAGGCAAGCGTTTTAACACCGCTGTGGGTGTATGTGGCGAGGCTGCAGCAGACCCATTGTTGGCATGTGT
CCTTACTGGTTTAGGCGTCAATTCGCTGTCAGCTGCATCGACGGCGATCGCGGGTGTGGGTGCACAATTGGCATCTGTTG
ATTTTGAAACATGTGTCCGTGCTGCAGAAGCAGCTGTCAATGCAGAAGGTGCAATTGATGCACGAGCGGCTGTGCGTAAG
GTATTGCAGCCTTAA

Upstream 100 bases:

>100_bases
TGCATGTCAACATTTGCTTGTTGGATTGGTTGCGGAAATGTTGATTTTGTGGTGATGTAGAAGTGGTATTGACTACTCCC
GTGAGGATAGGTGGCGAACG

Downstream 100 bases:

>100_bases
GCTCGAGCAACGTTAATGAAGTGGGGCGGGAAGAACAAAAGTTCTTCCCGCCCCACTTCATTAATTATGATGAGAAAACT
TGAAAGATTGAAGCTAGGCG

Product: phosphoenolpyruvate:sugar phosphotransferase system enzyme I (PTS system)

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MNNVMTDTIIKGTGVVSGVRYAKAVWISPRPKLPQAGEVVDEADRAQQLDNFVAAADAVAQRLLDRSEHAEGAASEVLKA
TAGMVKDRGWQKAVKKGIQGGHPAEYAVVAATTKFVSMFEAAGGVMAERTTDLRDIRDRVIAELRGDLEPGLPDVHEQVV
LFADDLSPADTAALNTDYFVGLVTELGGPTSHTAIIARQLNVPCIVATGEKIHKIEVGTDVLIDGALGTVTLDADPETAR
AAELESRMLAERIAQWKGPAETKDGHRVQLLANVQDGKAARIAATESQAEGIGLFRTEMCFLTATEEPSVDEQAAVYKKV
LEQFPDSKVVVRSLDAGSDKPVAFASMADEMNPALGVRGLRVARANESLLTRQLDAIAQAAHELGRDENSPTWVMAPMVA
TTREARWFAGLCAERGLTAGAMIEVPAASLMADKLMPYLDFVSIGTNDLTQYTMAADRMSPQLAYLTDPWQPAVLRLIKH
TCEEGKRFNTAVGVCGEAAADPLLACVLTGLGVNSLSAASTAIAGVGAQLASVDFETCVRAAEAAVNAEGAIDARAAVRK
VLQP

Sequences:

>Translated_564_residues
MNNVMTDTIIKGTGVVSGVRYAKAVWISPRPKLPQAGEVVDEADRAQQLDNFVAAADAVAQRLLDRSEHAEGAASEVLKA
TAGMVKDRGWQKAVKKGIQGGHPAEYAVVAATTKFVSMFEAAGGVMAERTTDLRDIRDRVIAELRGDLEPGLPDVHEQVV
LFADDLSPADTAALNTDYFVGLVTELGGPTSHTAIIARQLNVPCIVATGEKIHKIEVGTDVLIDGALGTVTLDADPETAR
AAELESRMLAERIAQWKGPAETKDGHRVQLLANVQDGKAARIAATESQAEGIGLFRTEMCFLTATEEPSVDEQAAVYKKV
LEQFPDSKVVVRSLDAGSDKPVAFASMADEMNPALGVRGLRVARANESLLTRQLDAIAQAAHELGRDENSPTWVMAPMVA
TTREARWFAGLCAERGLTAGAMIEVPAASLMADKLMPYLDFVSIGTNDLTQYTMAADRMSPQLAYLTDPWQPAVLRLIKH
TCEEGKRFNTAVGVCGEAAADPLLACVLTGLGVNSLSAASTAIAGVGAQLASVDFETCVRAAEAAVNAEGAIDARAAVRK
VLQP
>Mature_564_residues
MNNVMTDTIIKGTGVVSGVRYAKAVWISPRPKLPQAGEVVDEADRAQQLDNFVAAADAVAQRLLDRSEHAEGAASEVLKA
TAGMVKDRGWQKAVKKGIQGGHPAEYAVVAATTKFVSMFEAAGGVMAERTTDLRDIRDRVIAELRGDLEPGLPDVHEQVV
LFADDLSPADTAALNTDYFVGLVTELGGPTSHTAIIARQLNVPCIVATGEKIHKIEVGTDVLIDGALGTVTLDADPETAR
AAELESRMLAERIAQWKGPAETKDGHRVQLLANVQDGKAARIAATESQAEGIGLFRTEMCFLTATEEPSVDEQAAVYKKV
LEQFPDSKVVVRSLDAGSDKPVAFASMADEMNPALGVRGLRVARANESLLTRQLDAIAQAAHELGRDENSPTWVMAPMVA
TTREARWFAGLCAERGLTAGAMIEVPAASLMADKLMPYLDFVSIGTNDLTQYTMAADRMSPQLAYLTDPWQPAVLRLIKH
TCEEGKRFNTAVGVCGEAAADPLLACVLTGLGVNSLSAASTAIAGVGAQLASVDFETCVRAAEAAVNAEGAIDARAAVRK
VLQP

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=443, Percent_Identity=33.1828442437923, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI48994992, Length=511, Percent_Identity=29.3542074363992, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1789193, Length=514, Percent_Identity=30.3501945525292, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1788726, Length=523, Percent_Identity=28.1070745697897, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787994, Length=385, Percent_Identity=25.7142857142857, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59808; Mature: 59808

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVMTDTIIKGTGVVSGVRYAKAVWISPRPKLPQAGEVVDEADRAQQLDNFVAAADAVA
CCCCCCHHHCCCCCHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QRLLDRSEHAEGAASEVLKATAGMVKDRGWQKAVKKGIQGGHPAEYAVVAATTKFVSMFE
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHH
AAGGVMAERTTDLRDIRDRVIAELRGDLEPGLPDVHEQVVLFADDLSPADTAALNTDYFV
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCCCHHHHCCCHHHH
GLVTELGGPTSHTAIIARQLNVPCIVATGEKIHKIEVGTDVLIDGALGTVTLDADPETAR
HHHHHCCCCCCHHHHEEEECCCCEEEECCCCEEEEECCCCEEEECCCEEEEECCCCCHHH
AAELESRMLAERIAQWKGPAETKDGHRVQLLANVQDGKAARIAATESQAEGIGLFRTEMC
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEE
FLTATEEPSVDEQAAVYKKVLEQFPDSKVVVRSLDAGSDKPVAFASMADEMNPALGVRGL
EEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHCCHHHCCCCE
RVARANESLLTRQLDAIAQAAHELGRDENSPTWVMAPMVATTREARWFAGLCAERGLTAG
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCC
AMIEVPAASLMADKLMPYLDFVSIGTNDLTQYTMAADRMSPQLAYLTDPWQPAVLRLIKH
CEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
TCEEGKRFNTAVGVCGEAAADPLLACVLTGLGVNSLSAASTAIAGVGAQLASVDFETCVR
HHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCHHHHHH
AAEAAVNAEGAIDARAAVRKVLQP
HHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNNVMTDTIIKGTGVVSGVRYAKAVWISPRPKLPQAGEVVDEADRAQQLDNFVAAADAVA
CCCCCCHHHCCCCCHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QRLLDRSEHAEGAASEVLKATAGMVKDRGWQKAVKKGIQGGHPAEYAVVAATTKFVSMFE
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHH
AAGGVMAERTTDLRDIRDRVIAELRGDLEPGLPDVHEQVVLFADDLSPADTAALNTDYFV
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCCCHHHHCCCHHHH
GLVTELGGPTSHTAIIARQLNVPCIVATGEKIHKIEVGTDVLIDGALGTVTLDADPETAR
HHHHHCCCCCCHHHHEEEECCCCEEEECCCCEEEEECCCCEEEECCCEEEEECCCCCHHH
AAELESRMLAERIAQWKGPAETKDGHRVQLLANVQDGKAARIAATESQAEGIGLFRTEMC
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEE
FLTATEEPSVDEQAAVYKKVLEQFPDSKVVVRSLDAGSDKPVAFASMADEMNPALGVRGL
EEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHCCHHHCCCCE
RVARANESLLTRQLDAIAQAAHELGRDENSPTWVMAPMVATTREARWFAGLCAERGLTAG
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCC
AMIEVPAASLMADKLMPYLDFVSIGTNDLTQYTMAADRMSPQLAYLTDPWQPAVLRLIKH
CEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
TCEEGKRFNTAVGVCGEAAADPLLACVLTGLGVNSLSAASTAIAGVGAQLASVDFETCVR
HHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCHHHHHH
AAEAAVNAEGAIDARAAVRKVLQP
HHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]