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The map label for this gene is lexA

Identifier: 38234011

GI number: 38234011

Start: 1446632

End: 1447345

Strand: Direct

Name: lexA

Synonym: DIP1426

Alternate gene names: 38234011

Gene position: 1446632-1447345 (Clockwise)

Preceding gene: 38234008

Following gene: 38234012

Centisome position: 58.13

GC content: 50.7

Gene sequence:

>714_bases
ATGCCAGTGAAGGATTCATCTTCTAACAAGAAGAACCAAATAGGAAAACTTTCCGAACGACAACGTCGAATTTTAGAAGT
CATCACCGACGCAGTTTCTCTCCGTGGCTACCCCCCGAGCATTAGAGAAATTGGTGATGCTGCGGGCTTGCAATCCACGT
CATCCGTCGCGTATCAACTTAAAGAGCTGGAAAAGAAGGGCTACCTTCGCAGAGACCCTAATAAGCCACGCGCAGTTGAT
GTTCGTGCGCTTCCCGACCCGATCCCTTCAAAGCCTGGCCGTAAACCAGGGCCTAAAAAGTCTTCGGTTGCAATAAGTCC
CGATCCGGCGGAAACTTCGCCCACCAGCTTTGTACCAATAGTTGGGTCCATTGCAGCGGGCAATCCAATTCTCGCCGAAG
AGAACGTAGACGGTTACTTCCCTTTCCCTTCCGAAATCGTCGGAGACGGTGATCTTTTTATGCTCCAAGTAGAGGGCGAA
TCTATGCGCGATGCGGGGATCCTACATCACGACTGGGTTGTCGTCCGTTCCCAGCCCGTTGCAGAACAAGGGGAATTTGT
CGCCGCGCTTATTGAAGGTGAAGCAACGGTAAAAGAATTCCACTCAGATTCGTCTGGCGTGTGGCTTTTACCCCACAACG
ACGCATTTGATCCCATTCCGGCTGAACACGCAGAAATTATGGGCAAAGTAGTTTCTATCCTCCGAAAGCTCTAA

Upstream 100 bases:

>100_bases
TCATCCCCCCGATCATGATGGCAGCCCTTTCAAACACTGCCGATACGTTATTCGCAAATAAATCACAACAAATCACGACT
ACTTTGAGGAAATAATTCTT

Downstream 100 bases:

>100_bases
TCCATCCTCCGGTGAGGAACCCTAACCCCCTGTATATTGATCTGAGATATAAGGGGGTTTACACATTACGCACCCCCGAA
GAGGCCTAGAATTCGGTTTT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MPVKDSSSNKKNQIGKLSERQRRILEVITDAVSLRGYPPSIREIGDAAGLQSTSSVAYQLKELEKKGYLRRDPNKPRAVD
VRALPDPIPSKPGRKPGPKKSSVAISPDPAETSPTSFVPIVGSIAAGNPILAEENVDGYFPFPSEIVGDGDLFMLQVEGE
SMRDAGILHHDWVVVRSQPVAEQGEFVAALIEGEATVKEFHSDSSGVWLLPHNDAFDPIPAEHAEIMGKVVSILRKL

Sequences:

>Translated_237_residues
MPVKDSSSNKKNQIGKLSERQRRILEVITDAVSLRGYPPSIREIGDAAGLQSTSSVAYQLKELEKKGYLRRDPNKPRAVD
VRALPDPIPSKPGRKPGPKKSSVAISPDPAETSPTSFVPIVGSIAAGNPILAEENVDGYFPFPSEIVGDGDLFMLQVEGE
SMRDAGILHHDWVVVRSQPVAEQGEFVAALIEGEATVKEFHSDSSGVWLLPHNDAFDPIPAEHAEIMGKVVSILRKL
>Mature_236_residues
PVKDSSSNKKNQIGKLSERQRRILEVITDAVSLRGYPPSIREIGDAAGLQSTSSVAYQLKELEKKGYLRRDPNKPRAVDV
RALPDPIPSKPGRKPGPKKSSVAISPDPAETSPTSFVPIVGSIAAGNPILAEENVDGYFPFPSEIVGDGDLFMLQVEGES
MRDAGILHHDWVVVRSQPVAEQGEFVAALIEGEATVKEFHSDSSGVWLLPHNDAFDPIPAEHAEIMGKVVSILRKL

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=224, Percent_Identity=29.0178571428571, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_CORDI (P61607)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939778.1
- ProteinModelPortal:   P61607
- SMR:   P61607
- MEROPS:   S24.001
- GeneID:   2649700
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1426
- NMPDR:   fig|257309.1.peg.1369
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   P61607
- ProtClustDB:   PRK00215
- BioCyc:   CDIP257309:DIP1426-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25707; Mature: 25576

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: ACT_SITE 161-161 ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKDSSSNKKNQIGKLSERQRRILEVITDAVSLRGYPPSIREIGDAAGLQSTSSVAYQL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
KELEKKGYLRRDPNKPRAVDVRALPDPIPSKPGRKPGPKKSSVAISPDPAETSPTSFVPI
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEHHH
VGSIAAGNPILAEENVDGYFPFPSEIVGDGDLFMLQVEGESMRDAGILHHDWVVVRSQPV
HHHHCCCCCEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCEEECEEEEECCCC
AEQGEFVAALIEGEATVKEFHSDSSGVWLLPHNDAFDPIPAEHAEIMGKVVSILRKL
CCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PVKDSSSNKKNQIGKLSERQRRILEVITDAVSLRGYPPSIREIGDAAGLQSTSSVAYQL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
KELEKKGYLRRDPNKPRAVDVRALPDPIPSKPGRKPGPKKSSVAISPDPAETSPTSFVPI
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEHHH
VGSIAAGNPILAEENVDGYFPFPSEIVGDGDLFMLQVEGESMRDAGILHHDWVVVRSQPV
HHHHCCCCCEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCEEECEEEEECCCC
AEQGEFVAALIEGEATVKEFHSDSSGVWLLPHNDAFDPIPAEHAEIMGKVVSILRKL
CCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910