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The map label for this gene is dut

Identifier: 38233985

GI number: 38233985

Start: 1419195

End: 1419653

Strand: Reverse

Name: dut

Synonym: DIP1400

Alternate gene names: 38233985

Gene position: 1419653-1419195 (Counterclockwise)

Preceding gene: 38233987

Following gene: 38233984

Centisome position: 57.05

GC content: 49.67

Gene sequence:

>459_bases
ATGGAAAACGACCAGGAAAAGCCAAGCATGGTGGCAATTCAACGCTTAGACCCTGAGCTTCCGCTGCCGGTTCGCAAGCA
TCGCGGTGACGCCGGCGCTGATCTATTTTCGGCTGAAAGCGTCACAATCGAACCAGGACATCGGATTCTTGTCGGAACCG
GCATAGCGATTGCACTGCCAATAGGTACTGTTGGTCTTATTCACCCTCGTTCTGGTCGTGCTTTGAAAGAGGGCCTGAGT
ATCGTCAATACCCCAGGTACTATCGATGCTGATTATCGCGGTGAGATTAAAGTTTGCCTGATTAATTTGGATCCCACTAC
TCCCATCCGTATTGAGCGAGGGGAAAGGATTGCACAATTACTGGTGCAAAAGGTTGAGCTGGTTGATTTCTGCGAAGTTG
AAACATTGTCAGAAACCGAGCGTGGTGTCAACGGTTACGGCTCTACTGGAGTTAACTGA

Upstream 100 bases:

>100_bases
CTTTCTGAACTTAGAAGCAACCTTTGTTATCTTGATAGTTCGTCATAACTCGTATTAGTTAAAGTTCGACTGCTCGAGCT
GAACGTAAGGAAGATACCGA

Downstream 100 bases:

>100_bases
GACTAGAATCTGTGAAGCAACACCAAAACTTCTTTAACAATCAACCAACGGAGTAATTATGTGGCCTTTTAAACGCCAAA
ATGGTGATTCTGACCATGAT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MENDQEKPSMVAIQRLDPELPLPVRKHRGDAGADLFSAESVTIEPGHRILVGTGIAIALPIGTVGLIHPRSGRALKEGLS
IVNTPGTIDADYRGEIKVCLINLDPTTPIRIERGERIAQLLVQKVELVDFCEVETLSETERGVNGYGSTGVN

Sequences:

>Translated_152_residues
MENDQEKPSMVAIQRLDPELPLPVRKHRGDAGADLFSAESVTIEPGHRILVGTGIAIALPIGTVGLIHPRSGRALKEGLS
IVNTPGTIDADYRGEIKVCLINLDPTTPIRIERGERIAQLLVQKVELVDFCEVETLSETERGVNGYGSTGVN
>Mature_152_residues
MENDQEKPSMVAIQRLDPELPLPVRKHRGDAGADLFSAESVTIEPGHRILVGTGIAIALPIGTVGLIHPRSGRALKEGLS
IVNTPGTIDADYRGEIKVCLINLDPTTPIRIERGERIAQLLVQKVELVDFCEVETLSETERGVNGYGSTGVN

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=140, Percent_Identity=40.7142857142857, Blast_Score=99, Evalue=1e-21,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=40.7142857142857, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=40.7142857142857, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1790071, Length=147, Percent_Identity=35.3741496598639, Blast_Score=80, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71988561, Length=140, Percent_Identity=42.1428571428571, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6319729, Length=142, Percent_Identity=36.6197183098592, Blast_Score=91, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19921126, Length=130, Percent_Identity=36.1538461538462, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583610, Length=130, Percent_Identity=36.1538461538462, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_CORDI (P61907)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939752.1
- ProteinModelPortal:   P61907
- SMR:   P61907
- GeneID:   2649475
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1400
- NMPDR:   fig|257309.1.peg.1343
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- PhylomeDB:   P61907
- ProtClustDB:   PRK00601
- BioCyc:   CDIP257309:DIP1400-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16404; Mature: 16404

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDQEKPSMVAIQRLDPELPLPVRKHRGDAGADLFSAESVTIEPGHRILVGTGIAIALP
CCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCEECCCEEEECCCCEEEEECCEEEEEC
IGTVGLIHPRSGRALKEGLSIVNTPGTIDADYRGEIKVCLINLDPTTPIRIERGERIAQL
CCEEEEEECCCCCHHHHCCEEECCCCCCCCCCCCCEEEEEEECCCCCCEEECCCHHHHHH
LVQKVELVDFCEVETLSETERGVNGYGSTGVN
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MENDQEKPSMVAIQRLDPELPLPVRKHRGDAGADLFSAESVTIEPGHRILVGTGIAIALP
CCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCEECCCEEEECCCCEEEEECCEEEEEC
IGTVGLIHPRSGRALKEGLSIVNTPGTIDADYRGEIKVCLINLDPTTPIRIERGERIAQL
CCEEEEEECCCCCHHHHCCEEECCCCCCCCCCCCCEEEEEEECCCCCCEEECCCHHHHHH
LVQKVELVDFCEVETLSETERGVNGYGSTGVN
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910