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The map label for this gene is aspS

Identifier: 38233939

GI number: 38233939

Start: 1367810

End: 1369615

Strand: Reverse

Name: aspS

Synonym: DIP1353

Alternate gene names: 38233939

Gene position: 1369615-1367810 (Counterclockwise)

Preceding gene: 38233941

Following gene: 38233938

Centisome position: 55.03

GC content: 50.78

Gene sequence:

>1806_bases
GTGCACGTGTTGCGCACTCATCTCGCAGGTGATCTTGGTAAAGAGACCGCCGGCCAGACAGTCACCCTCACAGGTTGGGT
TTCACGCCGTCGAGATCATGGTGGTGTTATCTTCATCGATCTCCGTGATTCTTCCGGGCTGGTTCAGGTCGTTTTCCGTG
AAAACGATGTAGCTGAGCAGGCACACCACCTGCGGAGTGAATTCTGTATCAAGGTAACCGGTGAAGTAGAGGCTCGTCCA
GAAGGTTCCGAGAACCCCAATCTTGCTTCGGGTGCAATCGAGGTCAACGTCACTGACTTGGAAATCCTGAATGAAGCTGC
ACCATTGCCATTCCAAATTGATGATGTTTCTCAGGGGGGCGAAGTTGGAGAAGAGACACGCCTTAAGTATCGTTACCTAG
ACTTGCGTCGCCCGAACCAAGGTGCAGCCTTGAGGTTGCGGTCGCAAGCTAATAAAGCGGCACGTAACGTACTCGATTCT
CACGATTTTGTTGAAATTGAGACGCCAACTCTGACGCGTTCGACTCCAGAAGGTGCCCGTGACTTTCTTGTCCCAGCTCG
TCTCAAGCCAGGATCATGGTATGCGCTACCTCAATCGCCGCAGCTATTCAAGCAGCTCTTGATGGTTGCGGGCATGGAGC
GCTACTACCAAATTGCTCGTTGTTATCGAGATGAGGATTTTCGCGCTGACCGTCAACCTGAATTTACTCAGCTCGATATC
GAGATGAGCTTTGTTGATCAAGATGATGTCATCGCATTGGCTGAAGAGATTGTTTCTTCGCTGTGGAAGCTCATCGGATA
TGAGATTCCTACACCGATTCCACGCATGACGTATGCCGATGCTATGCGTCTATATGGTTCGGACAAGCCCGATCTACGGT
TCGACATTAAGATCGTTGAGTGCACAGACTTCTTCAAGAACACCACTTTCCGAGTGTTCCAAAATGAATATGTCGGTGCA
GTTGTAATGGATGGTGGAGCTTCTCAGCCACGTCGTCAGCTTGATGCTTGGCAAGAATGGGCGAAACAGCGCGGAGCTAA
GGGACTCGCATACATTTTGGTCGGCGAGAATGGTGAGCTTACCGGCCCAGTTTCTAAGAACATCACTGACGAAGAACGTG
CTGGTATTGCAGCCCATGTTGGCGCAAAACCTGGCGATTGTATTTTCTTTGCTGCTGGTGAGACTAAGAGCTCACGCGCG
CTCTTAGGTGCTGCTCGAAACGAAATCGCTAAGAAACTTGACTTGATCAAGGAAGGCGATTGGGCATTTACTTGGGTCGT
AGATGCACCGTTGTTCGAGCCATCATCGGATGCAACTGCATCAGGAGACGTGGCACTTGGTCATTCGAAGTGGACTGCGG
TTCACCACGCATTTACTTCTCCGAAGCCTGAATGGCTAGATTCTTTTGACGAGAACCCAGGAGAAGCAACTGCTTATGCT
TACGACATTGTCTGCAACGGTAATGAAATCGGTGGCGGTTCGATTCGTATTCACCGTCGTGATGTTCAAGAACGAGTCTT
TAAAGTTATGGGCATCACGGAGGATGAAGCTCGCGAGAAGTTTGGTTTCTTGCTTGATGCCTTTGCCTTCGGTGCTCCTC
CACACGGCGGAATTGCTTTCGGTTGGGATCGTATCGTCTCGTTGCTTGGCGGATTCTCCTCAATTCGTGACGTAATCGCG
TTCCCGAAGTCAGGTGGCGGCGTTGATCCGCTAACCGACGCTCCCGCACCAATTCCCCTTGAGCAGCGTCGAGAAACTGG
CGTGGATTTCAAACCAAAGAAAAAGACTGACGAGTCCGCAGTCTAA

Upstream 100 bases:

>100_bases
CAAAAGGTGGGCTGGTGTCGCGAATATTACGGTAACGCGGTAAAATCGTTCAGTTGATAAGCAAAGGGCGGGCTAACTCC
CGTACAAGCGAAAGGATTTG

Downstream 100 bases:

>100_bases
CAATGCAAAGCTCACAGTGGGCTAGGCACTAGAAATTGGGGCTTTGTTGGCGTCATAATGATGCTAATGGGGTCCCGATT
TTCGCGAAAGCGGTATCGCT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MHVLRTHLAGDLGKETAGQTVTLTGWVSRRRDHGGVIFIDLRDSSGLVQVVFRENDVAEQAHHLRSEFCIKVTGEVEARP
EGSENPNLASGAIEVNVTDLEILNEAAPLPFQIDDVSQGGEVGEETRLKYRYLDLRRPNQGAALRLRSQANKAARNVLDS
HDFVEIETPTLTRSTPEGARDFLVPARLKPGSWYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDI
EMSFVDQDDVIALAEEIVSSLWKLIGYEIPTPIPRMTYADAMRLYGSDKPDLRFDIKIVECTDFFKNTTFRVFQNEYVGA
VVMDGGASQPRRQLDAWQEWAKQRGAKGLAYILVGENGELTGPVSKNITDEERAGIAAHVGAKPGDCIFFAAGETKSSRA
LLGAARNEIAKKLDLIKEGDWAFTWVVDAPLFEPSSDATASGDVALGHSKWTAVHHAFTSPKPEWLDSFDENPGEATAYA
YDIVCNGNEIGGGSIRIHRRDVQERVFKVMGITEDEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFSSIRDVIA
FPKSGGGVDPLTDAPAPIPLEQRRETGVDFKPKKKTDESAV

Sequences:

>Translated_601_residues
MHVLRTHLAGDLGKETAGQTVTLTGWVSRRRDHGGVIFIDLRDSSGLVQVVFRENDVAEQAHHLRSEFCIKVTGEVEARP
EGSENPNLASGAIEVNVTDLEILNEAAPLPFQIDDVSQGGEVGEETRLKYRYLDLRRPNQGAALRLRSQANKAARNVLDS
HDFVEIETPTLTRSTPEGARDFLVPARLKPGSWYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDI
EMSFVDQDDVIALAEEIVSSLWKLIGYEIPTPIPRMTYADAMRLYGSDKPDLRFDIKIVECTDFFKNTTFRVFQNEYVGA
VVMDGGASQPRRQLDAWQEWAKQRGAKGLAYILVGENGELTGPVSKNITDEERAGIAAHVGAKPGDCIFFAAGETKSSRA
LLGAARNEIAKKLDLIKEGDWAFTWVVDAPLFEPSSDATASGDVALGHSKWTAVHHAFTSPKPEWLDSFDENPGEATAYA
YDIVCNGNEIGGGSIRIHRRDVQERVFKVMGITEDEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFSSIRDVIA
FPKSGGGVDPLTDAPAPIPLEQRRETGVDFKPKKKTDESAV
>Mature_601_residues
MHVLRTHLAGDLGKETAGQTVTLTGWVSRRRDHGGVIFIDLRDSSGLVQVVFRENDVAEQAHHLRSEFCIKVTGEVEARP
EGSENPNLASGAIEVNVTDLEILNEAAPLPFQIDDVSQGGEVGEETRLKYRYLDLRRPNQGAALRLRSQANKAARNVLDS
HDFVEIETPTLTRSTPEGARDFLVPARLKPGSWYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDI
EMSFVDQDDVIALAEEIVSSLWKLIGYEIPTPIPRMTYADAMRLYGSDKPDLRFDIKIVECTDFFKNTTFRVFQNEYVGA
VVMDGGASQPRRQLDAWQEWAKQRGAKGLAYILVGENGELTGPVSKNITDEERAGIAAHVGAKPGDCIFFAAGETKSSRA
LLGAARNEIAKKLDLIKEGDWAFTWVVDAPLFEPSSDATASGDVALGHSKWTAVHHAFTSPKPEWLDSFDENPGEATAYA
YDIVCNGNEIGGGSIRIHRRDVQERVFKVMGITEDEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFSSIRDVIA
FPKSGGGVDPLTDAPAPIPLEQRRETGVDFKPKKKTDESAV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=615, Percent_Identity=40, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI45439306, Length=250, Percent_Identity=30, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI194272210, Length=252, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI5031815, Length=252, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI4758762, Length=285, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1788173, Length=585, Percent_Identity=47.3504273504273, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789256, Length=291, Percent_Identity=26.1168384879725, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790571, Length=296, Percent_Identity=26.3513513513513, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=611, Percent_Identity=34.6972176759411, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17551876, Length=282, Percent_Identity=28.7234042553192, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71984122, Length=285, Percent_Identity=27.719298245614, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=257, Percent_Identity=25.6809338521401, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=257, Percent_Identity=25.6809338521401, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=257, Percent_Identity=25.6809338521401, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=629, Percent_Identity=33.8632750397456, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6321807, Length=260, Percent_Identity=28.0769230769231, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=273, Percent_Identity=27.4725274725275, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324256, Length=273, Percent_Identity=23.8095238095238, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=25, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=37.6237623762376, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI17136276, Length=254, Percent_Identity=29.5275590551181, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19921528, Length=284, Percent_Identity=28.169014084507, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640849, Length=263, Percent_Identity=27.7566539923954, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24640851, Length=263, Percent_Identity=27.7566539923954, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CORDI (Q6NGZ4)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939706.1
- ProteinModelPortal:   Q6NGZ4
- SMR:   Q6NGZ4
- GeneID:   2648796
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1353
- NMPDR:   fig|257309.1.peg.1297
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q6NGZ4
- ProtClustDB:   PRK00476
- BioCyc:   CDIP257309:DIP1353-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66474; Mature: 66474

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLRTHLAGDLGKETAGQTVTLTGWVSRRRDHGGVIFIDLRDSSGLVQVVFRENDVAEQ
CCCHHHHHHCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEEEECCCCEEEEEEECCCHHHH
AHHLRSEFCIKVTGEVEARPEGSENPNLASGAIEVNVTDLEILNEAAPLPFQIDDVSQGG
HHHHHHCEEEEEECCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCEEECCCCCCC
EVGEETRLKYRYLDLRRPNQGAALRLRSQANKAARNVLDSHDFVEIETPTLTRSTPEGAR
CCCHHHEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCH
DFLVPARLKPGSWYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDI
HCEEECEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFVDQDDVIALAEEIVSSLWKLIGYEIPTPIPRMTYADAMRLYGSDKPDLRFDIKIVE
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEE
CTDFFKNTTFRVFQNEYVGAVVMDGGASQPRRQLDAWQEWAKQRGAKGLAYILVGENGEL
EHHHHCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
TGPVSKNITDEERAGIAAHVGAKPGDCIFFAAGETKSSRALLGAARNEIAKKLDLIKEGD
CCCCCCCCCCHHHCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
WAFTWVVDAPLFEPSSDATASGDVALGHSKWTAVHHAFTSPKPEWLDSFDENPGEATAYA
EEEEEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEE
YDIVCNGNEIGGGSIRIHRRDVQERVFKVMGITEDEAREKFGFLLDAFAFGAPPHGGIAF
EEEEECCCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
GWDRIVSLLGGFSSIRDVIAFPKSGGGVDPLTDAPAPIPLEQRRETGVDFKPKKKTDESA
CHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
V
C
>Mature Secondary Structure
MHVLRTHLAGDLGKETAGQTVTLTGWVSRRRDHGGVIFIDLRDSSGLVQVVFRENDVAEQ
CCCHHHHHHCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEEEECCCCEEEEEEECCCHHHH
AHHLRSEFCIKVTGEVEARPEGSENPNLASGAIEVNVTDLEILNEAAPLPFQIDDVSQGG
HHHHHHCEEEEEECCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCEEECCCCCCC
EVGEETRLKYRYLDLRRPNQGAALRLRSQANKAARNVLDSHDFVEIETPTLTRSTPEGAR
CCCHHHEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCH
DFLVPARLKPGSWYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDI
HCEEECEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFVDQDDVIALAEEIVSSLWKLIGYEIPTPIPRMTYADAMRLYGSDKPDLRFDIKIVE
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEE
CTDFFKNTTFRVFQNEYVGAVVMDGGASQPRRQLDAWQEWAKQRGAKGLAYILVGENGEL
EHHHHCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
TGPVSKNITDEERAGIAAHVGAKPGDCIFFAAGETKSSRALLGAARNEIAKKLDLIKEGD
CCCCCCCCCCHHHCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
WAFTWVVDAPLFEPSSDATASGDVALGHSKWTAVHHAFTSPKPEWLDSFDENPGEATAYA
EEEEEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEE
YDIVCNGNEIGGGSIRIHRRDVQERVFKVMGITEDEAREKFGFLLDAFAFGAPPHGGIAF
EEEEECCCEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
GWDRIVSLLGGFSSIRDVIAFPKSGGGVDPLTDAPAPIPLEQRRETGVDFKPKKKTDESA
CHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910