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The map label for this gene is aspA [H]

Identifier: 38233842

GI number: 38233842

Start: 1258020

End: 1259594

Strand: Reverse

Name: aspA [H]

Synonym: DIP1254

Alternate gene names: 38233842

Gene position: 1259594-1258020 (Counterclockwise)

Preceding gene: 38233843

Following gene: 38233841

Centisome position: 50.61

GC content: 49.65

Gene sequence:

>1575_bases
ATGGCTTCCCAAGCTAAAAACACAAAGGGCTCGAAAAACGCCGAGGTAACCAATGATGTTCTCGACGGAAAAACCGAAGG
CACCGCGGAGAAAGTAAACGATGCCCCCATTGCATCAGATGTAACAACAAAGCACGCCACTAAGAAAGCTGCACCAGGAT
TCCGTATCGAAGAAGACCTCTTGGGTGAGATGGAAGTTCCCGACGAGGTTTATTATGGCGTCCATACTCTTCGTGCCATC
GACAATTACAAGATCTCTAGCACCACCATTAATGATGTGCCAGAGTTCATTCGTGGCATGGTTCAGGTAAAAAAGGCCAC
CGCGCTTGCCAACCGACGACTCCATACTCTGCCGAAAGACAAGTGCGATGCCATTGTATGGGCTTGCGATCAAATCTTGG
ATCACGGTCGTTGCATGGATCAGTTCCCAATCGATGTGTTCCAAGGAGGCGCTGGCACAAGCGTCAACATGAACACTAAT
GAGGTAATCGCCAACCTCGCACTGGAGCATCTGGGTAAGCCAAAGGGATCCTATGACATCATCAACCCAAACGATGACGT
CAACATGAGCCAATCAACTAACGATGCCTACCCAACTGGATTCCGTTTGGGTGTCTATGAGGCGATGCAGGAACTGATCA
CTCGTATTGACAATCTACAGTTCGCATTCCATGCCAAAGGTGATGAGTTCACCGATATTCTGAAGATGGGTCGCACACAG
CTCCAAGATGCTGTACCGATGACTTTAGGCGAGGAATTCCAGGCATTCGCTCATAACTTGGCTGAGGAGCAAAGCGTTCT
GCGTATGGCTTCCGACCGGCTCAAGGAAGTTAACTTGGGCGCAACCGCTATCGGTACTGGCTTGAACACCCCAGCCGGTT
ACCGTTATCAGGTTGTAGCCGCATTGTCCGAGTGCACCGGCTTGGAAATTAAGTCTGCACGCGATCTTATTGAAGCTACT
TCAGACACTGGTGCGTATGTCCTTGCCCATGCAGCTGTTAAGCGTACTGCGATGAAGCTTTCTAAGATCTGCAATGATCT
TCGACTGCTCAGCTCCGGACCACGCGCAGGTCTCAACGAGATTAATCTTCCCGCTCGAGCTGCAGGTTCATCGATCATGC
CGGCAAAGGTCAACCCAGTTATCCCTGAGGTTGTTAACCAGGTTTGCTTCAAGGTTTTTGGTAACGATCTTACTGTCACC
ATGGCGGCAGAAGCTGGCCAGCTCCAGCTCAATGTGATGGAACCGGTTATCGGCCAAGCCTTGTTTGAGTCGATTCGAAT
TCTCGGTAATGCTGCAGTCACCCTTCAGCACAAGTGTGTCGAAGGGATTACCGCAAATGCTGACGTATGTCGCGCCTACG
TAGATAATTCCATTGGTATCGTTACCTATCTCAACCCTTTCATTGGTCACCATATGGGTGACGTGATTGGCAAGGAAGCT
GCTGAGACCGGCAAGTCCGTACGTGAGCTTGTATTGGAAAAGGGACTAATGGATGAAGAGACTTTGAACCAAATCCTGTC
CAAGGAAAATCTAATGCACCCTTCGTTTCGTGGAACCCTCTACCTAGATAACTAG

Upstream 100 bases:

>100_bases
AGCTATTAATTTCTAATTAATTATCGGGTGCATAATTCCTTATTTTGACGTACCCTTTAAATGTAAGAAATCGCCCACTC
ACTACAACAGGAGAGTTTAA

Downstream 100 bases:

>100_bases
TTTTTCAGTGGAATAAGCCTCGATACCCTAAACTGATAGTTCAAGGGTGTCGAGGCTTATTAATTTGCTCAGCCATCGAT
ACTCCTTTGTTAGTGCTTGT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MASQAKNTKGSKNAEVTNDVLDGKTEGTAEKVNDAPIASDVTTKHATKKAAPGFRIEEDLLGEMEVPDEVYYGVHTLRAI
DNYKISSTTINDVPEFIRGMVQVKKATALANRRLHTLPKDKCDAIVWACDQILDHGRCMDQFPIDVFQGGAGTSVNMNTN
EVIANLALEHLGKPKGSYDIINPNDDVNMSQSTNDAYPTGFRLGVYEAMQELITRIDNLQFAFHAKGDEFTDILKMGRTQ
LQDAVPMTLGEEFQAFAHNLAEEQSVLRMASDRLKEVNLGATAIGTGLNTPAGYRYQVVAALSECTGLEIKSARDLIEAT
SDTGAYVLAHAAVKRTAMKLSKICNDLRLLSSGPRAGLNEINLPARAAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVT
MAAEAGQLQLNVMEPVIGQALFESIRILGNAAVTLQHKCVEGITANADVCRAYVDNSIGIVTYLNPFIGHHMGDVIGKEA
AETGKSVRELVLEKGLMDEETLNQILSKENLMHPSFRGTLYLDN

Sequences:

>Translated_524_residues
MASQAKNTKGSKNAEVTNDVLDGKTEGTAEKVNDAPIASDVTTKHATKKAAPGFRIEEDLLGEMEVPDEVYYGVHTLRAI
DNYKISSTTINDVPEFIRGMVQVKKATALANRRLHTLPKDKCDAIVWACDQILDHGRCMDQFPIDVFQGGAGTSVNMNTN
EVIANLALEHLGKPKGSYDIINPNDDVNMSQSTNDAYPTGFRLGVYEAMQELITRIDNLQFAFHAKGDEFTDILKMGRTQ
LQDAVPMTLGEEFQAFAHNLAEEQSVLRMASDRLKEVNLGATAIGTGLNTPAGYRYQVVAALSECTGLEIKSARDLIEAT
SDTGAYVLAHAAVKRTAMKLSKICNDLRLLSSGPRAGLNEINLPARAAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVT
MAAEAGQLQLNVMEPVIGQALFESIRILGNAAVTLQHKCVEGITANADVCRAYVDNSIGIVTYLNPFIGHHMGDVIGKEA
AETGKSVRELVLEKGLMDEETLNQILSKENLMHPSFRGTLYLDN
>Mature_523_residues
ASQAKNTKGSKNAEVTNDVLDGKTEGTAEKVNDAPIASDVTTKHATKKAAPGFRIEEDLLGEMEVPDEVYYGVHTLRAID
NYKISSTTINDVPEFIRGMVQVKKATALANRRLHTLPKDKCDAIVWACDQILDHGRCMDQFPIDVFQGGAGTSVNMNTNE
VIANLALEHLGKPKGSYDIINPNDDVNMSQSTNDAYPTGFRLGVYEAMQELITRIDNLQFAFHAKGDEFTDILKMGRTQL
QDAVPMTLGEEFQAFAHNLAEEQSVLRMASDRLKEVNLGATAIGTGLNTPAGYRYQVVAALSECTGLEIKSARDLIEATS
DTGAYVLAHAAVKRTAMKLSKICNDLRLLSSGPRAGLNEINLPARAAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVTM
AAEAGQLQLNVMEPVIGQALFESIRILGNAAVTLQHKCVEGITANADVCRAYVDNSIGIVTYLNPFIGHHMGDVIGKEAA
ETGKSVRELVLEKGLMDEETLNQILSKENLMHPSFRGTLYLDN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=38.961038961039, Blast_Score=321, Evalue=1e-87,
Organism=Escherichia coli, GI87082375, Length=470, Percent_Identity=67.0212765957447, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=35.7298474945534, Blast_Score=292, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17553882, Length=482, Percent_Identity=40.2489626556017, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI32565146, Length=329, Percent_Identity=40.4255319148936, Blast_Score=244, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=39.1397849462366, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24640179, Length=466, Percent_Identity=38.8412017167382, Blast_Score=324, Evalue=9e-89,
Organism=Drosophila melanogaster, GI24640177, Length=466, Percent_Identity=38.8412017167382, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=39.0455531453362, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=37.7155172413793, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24583245, Length=456, Percent_Identity=35.0877192982456, Blast_Score=285, Evalue=6e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 56870; Mature: 56739

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQAKNTKGSKNAEVTNDVLDGKTEGTAEKVNDAPIASDVTTKHATKKAAPGFRIEEDL
CCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHH
LGEMEVPDEVYYGVHTLRAIDNYKISSTTINDVPEFIRGMVQVKKATALANRRLHTLPKD
CCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCH
KCDAIVWACDQILDHGRCMDQFPIDVFQGGAGTSVNMNTNEVIANLALEHLGKPKGSYDI
HHHHHHHHHHHHHHCCCCCHHCCHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEC
INPNDDVNMSQSTNDAYPTGFRLGVYEAMQELITRIDNLQFAFHAKGDEFTDILKMGRTQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
LQDAVPMTLGEEFQAFAHNLAEEQSVLRMASDRLKEVNLGATAIGTGLNTPAGYRYQVVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHH
ALSECTGLEIKSARDLIEATSDTGAYVLAHAAVKRTAMKLSKICNDLRLLSSGPRAGLNE
HHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
INLPARAAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVTMAAEAGQLQLNVMEPVIGQA
CCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHHHH
LFESIRILGNAAVTLQHKCVEGITANADVCRAYVDNSIGIVTYLNPFIGHHMGDVIGKEA
HHHHHHHHCCHHEEEHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AETGKSVRELVLEKGLMDEETLNQILSKENLMHPSFRGTLYLDN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
ASQAKNTKGSKNAEVTNDVLDGKTEGTAEKVNDAPIASDVTTKHATKKAAPGFRIEEDL
CCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHH
LGEMEVPDEVYYGVHTLRAIDNYKISSTTINDVPEFIRGMVQVKKATALANRRLHTLPKD
CCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCH
KCDAIVWACDQILDHGRCMDQFPIDVFQGGAGTSVNMNTNEVIANLALEHLGKPKGSYDI
HHHHHHHHHHHHHHCCCCCHHCCHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEC
INPNDDVNMSQSTNDAYPTGFRLGVYEAMQELITRIDNLQFAFHAKGDEFTDILKMGRTQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
LQDAVPMTLGEEFQAFAHNLAEEQSVLRMASDRLKEVNLGATAIGTGLNTPAGYRYQVVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHH
ALSECTGLEIKSARDLIEATSDTGAYVLAHAAVKRTAMKLSKICNDLRLLSSGPRAGLNE
HHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
INLPARAAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVTMAAEAGQLQLNVMEPVIGQA
CCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHHHH
LFESIRILGNAAVTLQHKCVEGITANADVCRAYVDNSIGIVTYLNPFIGHHMGDVIGKEA
HHHHHHHHCCHHEEEHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AETGKSVRELVLEKGLMDEETLNQILSKENLMHPSFRGTLYLDN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7789816; 12948626 [H]