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The map label for this gene is pafA

Identifier: 38233833

GI number: 38233833

Start: 1247794

End: 1249233

Strand: Reverse

Name: pafA

Synonym: DIP1245

Alternate gene names: 38233833

Gene position: 1249233-1247794 (Counterclockwise)

Preceding gene: 38233834

Following gene: 38233832

Centisome position: 50.2

GC content: 47.36

Gene sequence:

>1440_bases
GTGGGCGAGTACGAACGCACAGTTTTTACACGACGAATCACGGGCGTAGAAACCGAATATGGAATCACTTGTGTCGGCGA
CAACAGTCGCCGGCGCCTCGGTGCGGATGAGGCAGCACGCTATATGTTCCGTCCCGTTGTGGAGGAATGGGGAAGCTCCA
ACGTGTTTATCCCCAATGGAGCACGGCTATACTTGGACGTGGGTTCCCACCCTGAATATGCTTCTGCCGAGTGTGATTCA
CTTTCCCAGTTAATCGCCTATGACCGTGCTGGCGATAAAATTGTTGATCAATTAGCGCAACGGGCGGAAACTGCACTAGC
AACAGAGGGAATTGGGGGCAGAGTTTATCTATTTAAGAATAATCTTGATTCACTCGGAAATTCTTACGGCTGTCACGAGA
ATTATCTTGTAAGCAGAGACGTTGTCCTCAAAACGCTGGGAAGACAACTTCTACCATTTTTGATTACCCGTCAACTGATA
TGCGGTGCTGGATCCATCCAAGATGGGCAATTTCAAGTCTCTCAACGAGCTGATCATGTATGGGAAGGCGTATCTAGCGC
TACGACTAGGTCCCGCCCGATTATTAATACTCGAGATGAACCACACGCAGATTCTCATCGCTTTCGAAGGCTCCATGTAA
TCGTCGGTGATTCAAACATGTCTGAGACAACATGTGCGTTGAAGATAGGCTCGACTCAACTGGTCCTTGAGATGATTGAA
GCTGGTGCTTTAAGTCATGACCTTGAGCTAAGCAATGAAATTGCCGCAATTAGGGAAATCTCTCGAGATATTACTGGCAT
GGCTCCAGTACCGTTGAAAGCTGGAACATCGATGCCTGCCATTGAAATTCAGCGTCGATATGCCGAAAAAGCTCTGTGCT
GGCTCGAGCAACGCGGTGATACAGAAGGAACGCCAAACGCTGAAATGCACAAGGTTGTGTCTTTGTGGCTCGATACAATA
TCTGCTATCGAATCAAATGATCTCCAGGCTCTATCTCGAGATATCGACTGGGCTATCAAACTATCACTATTACGCCGTGC
TCAGCGTCGCATTGGCTGCAGTGAGTCTGATTTTACTCATCCAAAATTGGCTCAATTAGATTTGGCTTACCACGATATTC
GCGCTGGTCGAGGTGTTTTTCCTGTTTTGGAATCGAAGCAGTTAGTCAATAGATGGATTAACGATACAGATATAGAACAG
GCCACACGCATTGCGCCGTCAACTACCCGCGCAGCTCTTCGTGGAGAATTTTTAACGGCTGCCAAGAAACTTCAAGCACC
CATATCTGCTGATTGGCTACGGCTTAAAGTGAACCGGCCAGAGCCTCAAATTATTGAGCTTACTGATCCTTTTGAAAACA
CAGACGATCGCGTGGACCAGCTCATTAGCTACATGCGAAATCATGCGGCTTCTTACAGCACAGATACTGCAATTTCATAA

Upstream 100 bases:

>100_bases
GCTGGAGTTGATGATCTTTTAGATGAAATTGACGGTCTTTTGGAATCAAACGCTGAAGAGTTCGTTCGTTCCTATGTTCA
GAAAGGTGGACAGTAAATCC

Downstream 100 bases:

>100_bases
GTTTGGATAACGTGGAGATTTGTCATGGGCAAAGATGTAGCGCTGGAAAGAATCGTGAACTTGACCTTTGCGTTTTTGCG
AGCCGAAACCCAAGGCAGAC

Product: hypothetical protein

Products: NA

Alternate protein names: Proteasome accessory factor A; Pup-conjugating enzyme

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MGEYERTVFTRRITGVETEYGITCVGDNSRRRLGADEAARYMFRPVVEEWGSSNVFIPNGARLYLDVGSHPEYASAECDS
LSQLIAYDRAGDKIVDQLAQRAETALATEGIGGRVYLFKNNLDSLGNSYGCHENYLVSRDVVLKTLGRQLLPFLITRQLI
CGAGSIQDGQFQVSQRADHVWEGVSSATTRSRPIINTRDEPHADSHRFRRLHVIVGDSNMSETTCALKIGSTQLVLEMIE
AGALSHDLELSNEIAAIREISRDITGMAPVPLKAGTSMPAIEIQRRYAEKALCWLEQRGDTEGTPNAEMHKVVSLWLDTI
SAIESNDLQALSRDIDWAIKLSLLRRAQRRIGCSESDFTHPKLAQLDLAYHDIRAGRGVFPVLESKQLVNRWINDTDIEQ
ATRIAPSTTRAALRGEFLTAAKKLQAPISADWLRLKVNRPEPQIIELTDPFENTDDRVDQLISYMRNHAASYSTDTAIS

Sequences:

>Translated_479_residues
MGEYERTVFTRRITGVETEYGITCVGDNSRRRLGADEAARYMFRPVVEEWGSSNVFIPNGARLYLDVGSHPEYASAECDS
LSQLIAYDRAGDKIVDQLAQRAETALATEGIGGRVYLFKNNLDSLGNSYGCHENYLVSRDVVLKTLGRQLLPFLITRQLI
CGAGSIQDGQFQVSQRADHVWEGVSSATTRSRPIINTRDEPHADSHRFRRLHVIVGDSNMSETTCALKIGSTQLVLEMIE
AGALSHDLELSNEIAAIREISRDITGMAPVPLKAGTSMPAIEIQRRYAEKALCWLEQRGDTEGTPNAEMHKVVSLWLDTI
SAIESNDLQALSRDIDWAIKLSLLRRAQRRIGCSESDFTHPKLAQLDLAYHDIRAGRGVFPVLESKQLVNRWINDTDIEQ
ATRIAPSTTRAALRGEFLTAAKKLQAPISADWLRLKVNRPEPQIIELTDPFENTDDRVDQLISYMRNHAASYSTDTAIS
>Mature_478_residues
GEYERTVFTRRITGVETEYGITCVGDNSRRRLGADEAARYMFRPVVEEWGSSNVFIPNGARLYLDVGSHPEYASAECDSL
SQLIAYDRAGDKIVDQLAQRAETALATEGIGGRVYLFKNNLDSLGNSYGCHENYLVSRDVVLKTLGRQLLPFLITRQLIC
GAGSIQDGQFQVSQRADHVWEGVSSATTRSRPIINTRDEPHADSHRFRRLHVIVGDSNMSETTCALKIGSTQLVLEMIEA
GALSHDLELSNEIAAIREISRDITGMAPVPLKAGTSMPAIEIQRRYAEKALCWLEQRGDTEGTPNAEMHKVVSLWLDTIS
AIESNDLQALSRDIDWAIKLSLLRRAQRRIGCSESDFTHPKLAQLDLAYHDIRAGRGVFPVLESKQLVNRWINDTDIEQA
TRIAPSTTRAALRGEFLTAAKKLQAPISADWLRLKVNRPEPQIIELTDPFENTDDRVDQLISYMRNHAASYSTDTAIS

Specific function: Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAFA_CORDI (Q6NH95)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939600.1
- GeneID:   2649800
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1245
- NMPDR:   fig|257309.1.peg.1191
- HOGENOM:   HBG292322
- OMA:   EYATPEC
- ProtClustDB:   CLSK872004
- BioCyc:   CDIP257309:DIP1245-MONOMER
- HAMAP:   MF_02111
- InterPro:   IPR022279
- InterPro:   IPR004347
- PIRSF:   PIRSF018077
- TIGRFAMs:   TIGR03686

Pfam domain/function: PF03136 DUF245

EC number: NA

Molecular weight: Translated: 53461; Mature: 53330

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEYERTVFTRRITGVETEYGITCVGDNSRRRLGADEAARYMFRPVVEEWGSSNVFIPNG
CCCHHHHHHHHHHCCCCCCCCEEEECCCCCHHCCHHHHHHHHHHHHHHHCCCCCEEECCC
ARLYLDVGSHPEYASAECDSLSQLIAYDRAGDKIVDQLAQRAETALATEGIGGRVYLFKN
CEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
NLDSLGNSYGCHENYLVSRDVVLKTLGRQLLPFLITRQLICGAGSIQDGQFQVSQRADHV
CHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHH
WEGVSSATTRSRPIINTRDEPHADSHRFRRLHVIVGDSNMSETTCALKIGSTQLVLEMIE
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCHHHHHHHHH
AGALSHDLELSNEIAAIREISRDITGMAPVPLKAGTSMPAIEIQRRYAEKALCWLEQRGD
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TEGTPNAEMHKVVSLWLDTISAIESNDLQALSRDIDWAIKLSLLRRAQRRIGCSESDFTH
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
PKLAQLDLAYHDIRAGRGVFPVLESKQLVNRWINDTDIEQATRIAPSTTRAALRGEFLTA
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
AKKLQAPISADWLRLKVNRPEPQIIELTDPFENTDDRVDQLISYMRNHAASYSTDTAIS
HHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GEYERTVFTRRITGVETEYGITCVGDNSRRRLGADEAARYMFRPVVEEWGSSNVFIPNG
CCHHHHHHHHHHCCCCCCCCEEEECCCCCHHCCHHHHHHHHHHHHHHHCCCCCEEECCC
ARLYLDVGSHPEYASAECDSLSQLIAYDRAGDKIVDQLAQRAETALATEGIGGRVYLFKN
CEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
NLDSLGNSYGCHENYLVSRDVVLKTLGRQLLPFLITRQLICGAGSIQDGQFQVSQRADHV
CHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHH
WEGVSSATTRSRPIINTRDEPHADSHRFRRLHVIVGDSNMSETTCALKIGSTQLVLEMIE
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCHHHHHHHHH
AGALSHDLELSNEIAAIREISRDITGMAPVPLKAGTSMPAIEIQRRYAEKALCWLEQRGD
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TEGTPNAEMHKVVSLWLDTISAIESNDLQALSRDIDWAIKLSLLRRAQRRIGCSESDFTH
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
PKLAQLDLAYHDIRAGRGVFPVLESKQLVNRWINDTDIEQATRIAPSTTRAALRGEFLTA
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
AKKLQAPISADWLRLKVNRPEPQIIELTDPFENTDDRVDQLISYMRNHAASYSTDTAIS
HHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910