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The map label for this gene is aacA

Identifier: 14141849

GI number: 14141849

Start: 17224

End: 18663

Strand: Reverse

Name: aacA

Synonym: SAVP026

Alternate gene names: 14141849

Gene position: 18663-17224 (Counterclockwise)

Preceding gene: 14141850

Following gene: 14141846

Centisome position: 74.33

GC content: 24.65

Gene sequence:

>1440_bases
ATGAATATAGTTGAAAATGAAATATGTATAAGAACTTTAATAGATGATGATTTTCCTTTGATGTTAAAATGGTTAACTGA
TGAAAGAGTATTAGAATTTTATGGTGGTAGAGATAAAAAATATACATTAGAATCATTAAAAAAACATTATACAGAGCCTT
GGGAAGATGAAGTTTTTAGAGTAATTATTGAATATAACAATGTTCCTATTGGATATGGACAAATATATAAAATGTATGAT
GAGTTATATACTGATTATCATTATCCAAAAACTGATGAGATAGTCTATGGTATGGATCAATTTATAGGAGAGCCAAATTA
TTGGAGTAAAGGAATTGGTACAAGATATATTAAATTGATTTTTGAATTTTTGAAAAAAGAAAGAAATGCTAATGCAGTTA
TTTTAGACCCTCATAAAAATAATCCAAGAGCAATAAGGGCATACCAAAAATCTGGTTTTAGAATTATTGAAGATTTGCCA
GAACATGAATTACACGAGGGCAAAAAAGAAGATTGTTATTTAATGGAATATAGATATGATGATAATGCCACAAATGTTAA
GGCAATGAAATATTTAATTGAGCATTACTTTGATAATTTCAAAGTAGATAGTATTGAAATAATCGGTAGTGGTTATGATA
GTGTGGCATATTTAGTTAATAATGAATACATTTTTAAAACAAAATTTAGTACTAATAAGAAAAAAGGTTATGCAAAAGAA
AAAGCAATATATAATTTTTTAAATACAAATTTAGAAACTAATGTAAAAATTCCTAATATTGAATATTCGTATATTAGTGA
TGAATTATCTATACTAGGTTATAAAGAAATTAAAGGAACTTTTTTAACACCAGAAATTTATTCTACTATGTCAGAAGAAG
AACAAAATTTGTTAAAACGAGATATTGCCAGTTTTTTAAGACAAATGCACGGTTTAGATTATACAGATATTAGTGAATGT
ACTATTGATAATAAACAAAATGTATTAGAAGAGTATATATTGTTGCGTGAAACTATTTATAATGATTTAACTGATATAGA
AAAAGATTATATAGAAAGTTTTATGGAAAGACTAAATGCAACAACAGTTTTTGAGGGTAAAAAGTGTTTATGCCATAATG
ATTTTAGTTGTAATCATCTATTGTTAGATGGCAATAATAGATTAACTGGAATAATTGATTTTGGAGATTCTGGAATTATA
GATGAATATTGTGATTTTATATACTTACTTGAAGATAGTGAAGAAGAAATAGGAACAAATTTTGGAGAAGATATATTAAG
AATGTATGGAAATATAGATATTGAGAAAGCAAAAGAATATCAAGATATAGTTGAAGAATATTATCCTATTGAAACTATTG
TTTATGGAATTAAAAATATTAAACAGGAATTTATCGAAAATGGTAGAAAAGAAATTTATAAAAGGACTTATAAAGATTGA

Upstream 100 bases:

>100_bases
TAATCCTAGAGCTTGCCATGTATATGAAAAATTAGGTGGTAAAAATGATTATAAAGATGAAATAGTATATGTATATGATT
ATGAAAAAGGTGATAAATAA

Downstream 100 bases:

>100_bases
TTATATAATATATGAAAAGCTATTATAAAAGACATTAGTATTAAATAGTTTAAAAAAATGAAAAATAATAAAGGAAGTGA
GTCAAGTCCAGACTCCTGTG

Product: N-acetyltransferase

Products: NA

Alternate protein names: 6'-aminoglycoside N-acetyltransferase; AAC(6'); 2''-aminoglycoside phosphotransferase; APH(2'')

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYD
ELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKE
KAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGII
DEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD

Sequences:

>Translated_479_residues
MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYD
ELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKE
KAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGII
DEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD
>Mature_479_residues
MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYD
ELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKE
KAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGII
DEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD

Specific function: Resistance to gentamicin, tobramycin, and kanamycin. Tobramycin and kanamycin resistance is due to the ACC activity, specified by N-terminal region, and the gentamicin resistance is due to the APH activity encoded by the C-terminal region of the protein

COG id: COG1670

COG function: function code J; Acetyltransferases, including N-acetylases of ribosomal proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AACA_ENTFA (P0A0C2)

Other databases:

- EMBL:   M13771
- EMBL:   AE016833
- PIR:   A26048
- RefSeq:   NP_816984.1
- ProteinModelPortal:   P0A0C2
- GeneID:   1202231
- GenomeReviews:   AE016833_GR
- KEGG:   efa:EFA0061
- TIGR:   EF_A0061
- HOGENOM:   HBG535725
- OMA:   REICIDK
- BioCyc:   EFAE226185:EF_A0061-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR002575
- InterPro:   IPR011009
- Gene3D:   G3DSA:3.40.630.30

Pfam domain/function: PF00583 Acetyltransf_1; PF01636 APH; SSF55729 Acyl_CoA_acyltransferase; SSF56112 Kinase_like

EC number: NA

Molecular weight: Translated: 56856; Mature: 56856

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS51186 GNAT

Important sites: BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLESLKKHYTEPWEDEVFR
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHEE
VIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLI
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHEECHHHHCCCCCCCCCCCHHHHHHHH
FEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYD
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCHHHHCCCHHHHCCCCCCEEEEEEEEC
DNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKE
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCHHH
KAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKR
HHHHHHHHCCCCCCEECCCCCHHHHCCHHHHEEHHHHCCCEECHHHHHHHHHHHHHHHHH
DIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNA
HHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTN
CEEECCCEEEECCCCCCCEEEEECCCCEEEEEECCCCCCHHHHCCEEEEEECCCHHHCCC
FGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLESLKKHYTEPWEDEVFR
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHEE
VIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLI
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHEECHHHHCCCCCCCCCCCHHHHHHHH
FEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYD
HHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCHHHHCCCHHHHCCCCCCEEEEEEEEC
DNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKE
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCHHH
KAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKR
HHHHHHHHCCCCCCEECCCCCHHHHCCHHHHEEHHHHCCCEECHHHHHHHHHHHHHHHHH
DIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNA
HHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTN
CEEECCCEEEECCCCCCCEEEEECCCCEEEEEECCCCCCHHHHCCEEEEEECCCHHHCCC
FGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3015884; 12663927