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The map label for this gene is pbuE [H]

Identifier: 15925442

GI number: 15925442

Start: 2588995

End: 2590182

Strand: Reverse

Name: pbuE [H]

Synonym: SAV2452

Alternate gene names: 15925442

Gene position: 2590182-2588995 (Counterclockwise)

Preceding gene: 15925443

Following gene: 15925439

Centisome position: 89.98

GC content: 35.27

Gene sequence:

>1188_bases
ATGTCAATCATGCGACTATTTACATTCATTTTAAGTATTTTTATCGTAGGAATGGTTGAAATGATGGTTGCAGGAATTAT
GAACTTGATGAGTCAGGACTTACATGTATCAGAAGCTGTCGTTGGTCAATTAGTGACAATGTACGCTTTAACATTTGCGA
TATGTGGACCTATTCTGGTTAAATTAACGAACCGTTTTTCATCAAGGCCTGTATTATTATGGACATTACTTATATTTATC
ATTGGTAATGGCATTATTGCTGTAGCGCCAAATTTTTCAATATTAGTAGTTGGTAGAATTATCTCATCTGCAGCAGCAGC
ACTAATTATCGTAAAAGTATTAGCTATTACAGCGATGTTATCAGCACCTAAAAATCGTGGCAAAATGATTGGACTTGTCT
ATACAGGGTTTAGTGGGGCCAATGTTTTTGGTGTACCAATTGGAACGGTTATCGGCGATTTAGTAGGTTGGCGCTATACA
TTTCTATTCTTAATTATTGTGAGTATTATTGTTGGCTTCTTGATGATGATCTATTTACCGAAGGATCAGGAAATACAACG
AGGCCCTGTGAATCATGAGGCACCATCTCATGAAAATCATGTTACTTCGAAAATATTAAGACCTGCTGAAGTAGCAAAAT
ATTTAATTATTACGTTTTTAGTATTGATTGCAAACTCAGTGACATTCGTCTTTATAAATCCACTTATTTTATCTAATGGT
CATGATATGTCATTCGTTTCATTAGCACTTCTAGTAAATGGAATCGCTGGCGTTATTGGAACATCATTGGGTGGTATATT
CTCCGATAAAATTACAAGTAAGCGTTGGTTAATGATTTCTGTTTCTATTTTTATCGTTATGATGTTACTTATGAATTTAA
TCTTACCTGGTTCAGGTCTATTGTTAGCAGGACTATTTATTTGGAATATCATGCAATGGAGTACTAATCCAGCAGTGCAA
AGCGGTGTGATTCAACATGTTGAAGGCGACACAAGCCAAGTAATGAGTTGGAACATGTCTAGTTTAAACGCTGGTATTGG
TGTTGGCGGCATTATTGGAGGCTTGGTCATGACACATGTTTCTGTTCAAGCTATCACATATACGAGTGCCATCATTGGCG
CATTAGGATTAATCGTTGTTTTCACATTGAAAAATAATCATTATGCTAAAACATTTAAATCATCATAA

Upstream 100 bases:

>100_bases
TAGAACGGTTATATATAAAATTGTGTTGCACACATTTTTTAATAAATCGTTATTCTAAGGGAAATGAATATCGGAAATTT
TGTTTGAAAGGAGTTTTAAA

Downstream 100 bases:

>100_bases
TTCTCATATGAAAAGCACGCCTGCTATCAAATTCAGGTGTGCTTTTTTAGATGCGATAACGTTATTGATATGTGCGATAA
TAGCGACGTTCATTATGATA

Product: chloramphenicol resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFI
IGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNG
HDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQ
SGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

Sequences:

>Translated_395_residues
MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFI
IGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNG
HDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQ
SGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS
>Mature_394_residues
SIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFII
GNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTF
LFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGH
DMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQS
GVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786595, Length=357, Percent_Identity=22.96918767507, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787947, Length=269, Percent_Identity=26.7657992565056, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693198, Length=247, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787808, Length=378, Percent_Identity=26.7195767195767, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42546; Mature: 42415

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAML
HHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLP
HCCCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNG
CCCCHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCC
HDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGL
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
LLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
SVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS
HHHHHHHHHHHHHHHHHHHEEEECCCCCCHHCCCC
>Mature Secondary Structure 
SIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAML
HHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLP
HCCCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNG
CCCCHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCC
HDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGL
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
LLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHV
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
SVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS
HHHHHHHHHHHHHHHHHHHEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]