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The map label for this gene is bioA [H]

Identifier: 15925416

GI number: 15925416

Start: 2555863

End: 2557221

Strand: Reverse

Name: bioA [H]

Synonym: SAV2426

Alternate gene names: 15925416

Gene position: 2557221-2555863 (Counterclockwise)

Preceding gene: 15925417

Following gene: 15925415

Centisome position: 88.84

GC content: 33.63

Gene sequence:

>1359_bases
ATGAATTACACACAACAACTTAAACAAAAAGACTCAGAATATGTTTGGCATCCATTTACACAAATGGGTGTATATAGCAA
AGAAGAAGCAATCATCATTGAAAAAGGAAAGGGTAGTTACCTTTACGATACGAATGGCAATAAATATTTAGATGGTTATG
CATCGTTGTGGGTCAATGTGCATGGTCATAATAACAAATACTTGAATAAGGTAATTAAAAAGCAACTCAATAAAATTGCC
CATTCTACGCTGCTAGGATCATCAAATATTCCGTCAATAGAACTTGCGGAAAAATTAATCGAAATCACGCCAAGTAATCT
AAGAAAAGTATTTTATTCTGATACAGGCAGTGCGTCTGTTGAAATCGCAATAAAGATGGCATATCAGTATTGGAAAAATA
TTGATAGAGAAAAATATGCCAAGAAAAACAAGTTTATAACGCTAAATCACGGTTATCATGGGGATACGATTGGTGCGGTA
AGTGTTGGTGGTATCAAGACCTTTCATAAAATATTTAAAGACTTAATATTTGAGAATATTCAAGTAGAAAGCCCATCTTT
CTATCGCAGTAATTACGATACTGAAAATGAAATTATGACAGCTATTTTAACGAATATAGAGCAAATTCTAATTGAAAGAA
ATGATGAAATCGCAGGGTTTATATTGGAACCGTTGATTCAAGGTGCGACAGGCTTGTTTGTTCATCCTAAAGGCTTTTTG
AAAGAAGTCGAGAAATTGTGCAAAAAATACGATGTCTTATTAATTTGTGATGAGGTAGCAGTTGGTTTTGGGAGAACTGG
AAAGATGTTTGCATGCAATCATGAAGATGTTCAACCGGATATTATGTGTTTAGGTAAGGCGATTACTGGTGGCTACTTAC
CACTTGCAGCTACATTGACATCTCAAAAAATATACAATACATTTTTAAGTGATTCGCATGGTGTTAATACCTTTTTCCAT
GGTCATACATACACCGGAAATCAAATCGTTTGTACGGTTGCATTAGAAAATATAAGACTTTATGAAAAACGTAAGTTATT
GTCACATATTGAAACGACATCATCAACACTTGAGAAACAGTTACATGCGCTGAAGCGTCATCGAAATGTTGGTGATGTAA
GAGGACGAGGCTTAATGTTTGGAGTTGAATTAGTTACAGATAAAGATAGTAAAACGCCGTTAGAAATTGAAAAAGTTGAA
CGTATTGTACGTAAATGTAAAGAAAACGGGCTAATGATTAGAAATTTAGAAAATGTCATTACGTTTGTGCCAGTGTTAAG
TATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTTAAAAAGGCAGTACATAACATTTTAGATAGGAAGTGTTAA

Upstream 100 bases:

>100_bases
ATTGGAAAATTAACAAATAAAACAGTCTATACATTTGAAGAACATGCCACGTATGAAAATTTCTCAGAAGCATTTTTAAA
GCAATTAATAGGAGTTAAAA

Downstream 100 bases:

>100_bases
TATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAAATTTATGAGGATACTAATA
TTGATACCTTAGATTTATTA

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIA
HSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSQKIYNTFLSDSHGVNTFFH
GHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVE
RIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

Sequences:

>Translated_452_residues
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIA
HSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSQKIYNTFLSDSHGVNTFFH
GHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVE
RIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC
>Mature_452_residues
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIA
HSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSQKIYNTFLSDSHGVNTFFH
GHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVE
RIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=448, Percent_Identity=28.5714285714286, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4557809, Length=416, Percent_Identity=28.6057692307692, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI37574042, Length=427, Percent_Identity=28.8056206088993, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI226442705, Length=426, Percent_Identity=28.6384976525822, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI24119277, Length=430, Percent_Identity=27.2093023255814, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI226442709, Length=388, Percent_Identity=28.8659793814433, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI284507298, Length=333, Percent_Identity=28.8288288288288, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI188536080, Length=335, Percent_Identity=23.2835820895522, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI38679950, Length=335, Percent_Identity=23.2835820895522, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI38679946, Length=335, Percent_Identity=23.2835820895522, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786991, Length=438, Percent_Identity=32.648401826484, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI145693181, Length=362, Percent_Identity=32.5966850828729, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788044, Length=430, Percent_Identity=28.8372093023256, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789759, Length=429, Percent_Identity=27.039627039627, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789016, Length=453, Percent_Identity=26.0485651214128, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=26.1282660332542, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564660, Length=448, Percent_Identity=29.6875, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25144271, Length=446, Percent_Identity=27.5784753363229, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI25144274, Length=243, Percent_Identity=34.5679012345679, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71992977, Length=412, Percent_Identity=24.7572815533981, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=315, Percent_Identity=25.0793650793651, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324386, Length=464, Percent_Identity=30.3879310344828, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324432, Length=438, Percent_Identity=29.6803652968037, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323470, Length=447, Percent_Identity=26.8456375838926, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6321456, Length=446, Percent_Identity=23.3183856502242, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357415, Length=422, Percent_Identity=29.1469194312796, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI161085790, Length=446, Percent_Identity=27.5784753363229, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI28574759, Length=451, Percent_Identity=27.4944567627494, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21356575, Length=443, Percent_Identity=24.8306997742664, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281366494, Length=452, Percent_Identity=24.7787610619469, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24667139, Length=452, Percent_Identity=24.7787610619469, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24667143, Length=452, Percent_Identity=24.7787610619469, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 51431; Mature: 51431

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNV
CCHHHHHHHCCCCEEECCHHHHCCCCCCCEEEEECCCCCEEEECCCCEEECCEEEEEEEE
HGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASV
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCEE
EIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENI
EEHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCC
QVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCHHHH
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLT
HHHHHHHHHCCEEEEECHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHH
SQKIYNTFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQ
HHHHHHHHHCCCCCCCEEECCEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRKCKENGLMIRNLENVI
HHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
TFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNV
CCHHHHHHHCCCCEEECCHHHHCCCCCCCEEEEECCCCCEEEECCCCEEECCEEEEEEEE
HGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASV
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHEEEECCCCCCEE
EIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENI
EEHHHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCC
QVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFL
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCHHHH
KEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLT
HHHHHHHHHCCEEEEECHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHH
SQKIYNTFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQ
HHHHHHHHHCCCCCCCEEECCEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
LHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRKCKENGLMIRNLENVI
HHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
TFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]