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The map label for this gene is narI [H]

Identifier: 15925384

GI number: 15925384

Start: 2520945

End: 2521622

Strand: Reverse

Name: narI [H]

Synonym: SAV2394

Alternate gene names: 15925384

Gene position: 2521622-2520945 (Counterclockwise)

Preceding gene: 15925385

Following gene: 15925383

Centisome position: 87.6

GC content: 32.45

Gene sequence:

>678_bases
ATGCTTAATCAGTTTTTATGGGTTATATTTCCGTATTTATGTTTAGTTGTCTTTGTCGCAGGTCATATTGCACGTTATCG
TTATGATAAATTTTCTTGGACAGCTAAATCTAGTGAACTAATAGAAAGAAAAAGGTTGATGTGGGGAAGTTTATTATTTC
ACTTAGGTATTATTCCAGTGTTTTTTGGACACGTAGTTGGATTGTTAATACCGAAATCATGGATGGATGCAGTGGGGGTA
TCTGAGCATTTATATCATATAGGCGCTGTTTATATTGGTAGCATCTTTGGTATAATAACTTTGATAGGGATGTTTTTATT
AACAGCGAGACGTGTTACTACGAAAAGTATTCGAAGATTAAGTTCAGCGTCGGATATATTTGTAAATTTCTTATTATTAC
TTATCGTATTTATGGGATGTTACGCAACATTAGTAACCAATATACAACATCCAGATTTTAACTACAGAACTTCATTATCA
ATTTGGTTTAGACAATTATTTATGTTAAAACCAGATGCGTCATTGATGAGTGGTGTGCCGATTGCATTTAAAATGCATAT
ATTGTTAGGTTTCACTATTATGGCTTGTTGGCCATTTACACGTTTGGTACATGTTTGGAGTGTACCTTTGACATATATAA
ATCGCAGATATATTATTTACCGTAAGAATAAAGTGTAA

Upstream 100 bases:

>100_bases
AATGCACTTGGTGAAGATAATAATCCATATGCCTATGTGATTCAAGCACTTAGAAAAACATTGAAATTATGTTTGACATC
ACCAAAAGGAGTGAAACATC

Downstream 100 bases:

>100_bases
GCATAAGAGGTGAAAGGATTTGACACCAGAAGCAATAATTGAAGATCGTAGATTTCAAGAGACATTAGATAAAATACGCA
AAGAAGAGGGTTATGATTTT

Product: nitrate reductase gamma chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGV
SEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV

Sequences:

>Translated_225_residues
MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGV
SEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV
>Mature_225_residues
MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGV
SEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Trans

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=224, Percent_Identity=37.5, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787480, Length=223, Percent_Identity=34.0807174887892, Blast_Score=137, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 26136; Mature: 26136

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRL
HHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLSIWFRQLFMLKPDASLMSGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCC
IAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
>Mature Secondary Structure
MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRL
HHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLSIWFRQLFMLKPDASLMSGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCC
IAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]