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The map label for this gene is narK

Identifier: 15925378

GI number: 15925378

Start: 2515310

End: 2516479

Strand: Reverse

Name: narK

Synonym: SAV2388

Alternate gene names: 15925378

Gene position: 2516479-2515310 (Counterclockwise)

Preceding gene: 15925381

Following gene: 15925375

Centisome position: 87.42

GC content: 34.44

Gene sequence:

>1170_bases
ATGTACAAAACAAAAGGTGGCTTTCAACTTACATTACAAACATTAAGTTTAGTGGTTGGGTTTATGGCTTGGAGTATAAT
TGCGCCATTAATGCCCTTTATTAAACAAGATGTCAATGTTACTGAAGGTCAAATATCAATCATTTTAGCGATACCAGTTA
TTTTGGGATCAGTGCTCCGTGTGCCATTTGGTTATTTAACAAATATTGTTGGTGCTAAATGGGTATTCTTTACAAGTTTT
ATCGTATTGTTATTCCCGATATTTTTCTTAAGCCAAGCACAAACGCCGGGTATGTTAATGGCATCAGGATTTTTCCTTGG
CGTAGGTGGCGCAATTTTCTCTGTTGGTGTTACATCAGTTCCTAAATATTTCCCAAAAGAAAAAGTAGGTCTAGCAAATG
GTATTTATGGTATGGGGAATATCGGTACAGCAGTTTCTTCATTTTTAGCACCACCGATAGCGGGTATTATTGGTTGGCAA
ACAACAGTTAGAAGTTACTTAATTATTATCGCTTTATTTGCATTAATTATGTTCATTTTTGGTGACACACAAGAACGTAA
AATTAAAGTGCCATTAATGGCCCAAATGAAAACATTATCTAAAAACTACAAATTATATTACTTAAGTTATTGGTATTTCA
TTACTTTTGGTGCTTTTGTAGCATTTGGTATTTTCTTACCTAACTACTTAGTAAATCATTTTGGAATTGACAAAGTAGAT
GCTGGTATTCGTTCAGGTGTATTCATTGCGCTGGCAACATTCTTGAGACCAATAGGTGGTATACTAGGTGATAAATTTAA
TGCAGTTAAAGTACTGATGATTGATTTTGTTATTATGATTATCGGTGCCGTTATTTTAGGTATTTCAGACCATATCGCAT
TATTCACTGTAGGCTGTTTAACAATAAGTATTTGTGCAGGTATTGGTAACGGCTTAATTTTTAAATTAGTACCATCCTAC
TTCTCAAATGAAGCAGGATCAGCAAATGGTATCGTATCAATGATGGGTGGTTTAGGAGGATTCTTCCCACCGTTGGTCAT
CACATACGTAGCTAATTTAACAGGTTCAAGCCATTTAGCATTTATTTTCTTAGCGGTATTTGGATGCATTGCATTATTTA
CCATGCGCCATTTATATCAAAAAGAATATGGTTCATTGAAACACAGTTGA

Upstream 100 bases:

>100_bases
CATATAAATGAAAGCATTTTTAAATAACATTGACCTCTAATATATAGGCAGAGTATTGATATCTATTAAAAAATAAATGA
TTTTGATGAAGGTGAAACGT

Downstream 100 bases:

>100_bases
TATGTAATACATGACATTCATTTAGTTAAATACAAAGCCTTAATATCATGCACAATATTCGTAGCATGACATTAAGGCTT
TAGTAGTGTATTCATTTTAA

Product: nitrite extrusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSF
IVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVD
AGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSY
FSNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS

Sequences:

>Translated_389_residues
MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSF
IVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVD
AGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSY
FSNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS
>Mature_389_residues
MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSF
IVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVD
AGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSY
FSNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS

Specific function: Probably required for nitrate uptake under anoxic conditions. Also possibly involved in excretion of nitrite produced by the dissimilatory reduction of nitrate

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family

Homologues:

Organism=Escherichia coli, GI1787475, Length=398, Percent_Identity=24.8743718592965, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787743, Length=403, Percent_Identity=23.8213399503722, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367193, Length=428, Percent_Identity=21.2616822429907, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NART_STAA1 (A7X618)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442962.1
- ProteinModelPortal:   A7X618
- STRING:   A7X618
- EnsemblBacteria:   EBSTAT00000004001
- GeneID:   5561430
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2372
- eggNOG:   COG2223
- GeneTree:   EBGT00050000023927
- HOGENOM:   HBG479449
- OMA:   SSHIALF
- ProtClustDB:   CLSK885946
- BioCyc:   SAUR418127:SAHV_2372-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 42176; Mature: 42176

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1090351c)-; HASH(0x103a2660)-; HASH(0xb767d4c)-; HASH(0x102f7474)-; HASH(0xffe875c)-; HASH(0xdaa8ef8)-; HASH(0x10646718)-; HASH(0x100b3b28)-; HASH(0x107b82f0)-; HASH(0x106284d0)-; HASH(0xba83590)-; HASH(0x1096e024)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA