Click here to switch to the map view.

The map label for this gene is lacG

Identifier: 15925179

GI number: 15925179

Start: 2332157

End: 2333569

Strand: Reverse

Name: lacG

Synonym: SAV2189

Alternate gene names: 15925179

Gene position: 2333569-2332157 (Counterclockwise)

Preceding gene: 15925180

Following gene: 15925178

Centisome position: 81.07

GC content: 34.75

Gene sequence:

>1413_bases
ATGACTAAAACATTACCTGAAGATTTTATTTTTGGTGGAGCAACAGCAGCATATCAAGCAGAAGGTGCGACAAATACAGA
TGGTAAAGGTCGTGTTGCATGGGATACGTATTTGGAAGAAAACTATTGGTACACTGCAGAACCAGCGAGTGATTTTTATA
ACAGATATCCAGTGGATTTAGAATTAAGTGAAAAATTCGGTGTTAATGGTATCCGTATTTCAATTGCATGGTCACGTATC
TTCCCAAATGGATATGGCGAAGTTAACCCTAAAGGTGTTGAGTATTACCATAAGTTATTTGCAGAGTGTCATAAACGTCA
TGTTGAACCATTTGTAACATTGCATCATTTTGATACACCAGAAGTGTTACACAAAGATGGCGATTTTCTAAATCGTAAAA
CGATTGACTACTTTGTAGATTATGCTGAATATTGTTTTAAAGAATTCCCAGAAGTAAAATATTGGACTACATTCAATGAA
ATTGGACCTATTGGTGATGGTCAATATTTAGTAGGTAAATTCCCTCCAGGTATTAAATATGATTTTGAAAAAGTTTTCCA
ATCACATCACAATATGATGGTTGCTCATGCAAGAGCAGTAAAATTATTTAAAGATGGCGGCTATCAAGGAGAAATTGGTG
TTGTACACGCATTGCCAACAAAATATCCGTTCGATCCATCAAATCCTGAAGATGTTCGTGCAGCAGAATTAGAAGATATC
ATCCATAATAAATTCATCTTAGATGCAACATATTTAGGTAAGTATTCTCGTGAAACGATGGAAGGTGTGCAACATATCTT
ATCTGTTAATGGCGGTAAATTAAATATTACAGATGAAGATTATGCCATTTTAGATGCAGCCAAAGATTTAAACGACTTCT
TAGGTATCAATTACTACATGAGTGACTGGATGAGAGGTTACGATGGAGAATCTGAAATCACGCATAATGCGACAGGTGAT
AAAGGCGGTTCTAAATACCAACTTAAAGGTGTCGGACAACGTGAATTTGATGTCGACGTACCTCGCACAGACTGGGACTG
GATGATCTATCCTCAAGGTTTATATGATCAAATCATGCGTGTTGTTAAAGATTATCCTAACTATCACAAGATTTATATCA
CTGAAAATGGTTTAGGATATAAAGATGAATTTATTGAATCTGAAAAAACAGTTCATGATGATGCACGTATTGATTATGTA
AGACAACATTTGAACGTTATTGCAGATGCTATTAAAGATGGTGCAAATGTTAAAGGTTACTTTATTTGGTCATTAATGGA
TGTGTTCTCTTGGTCAAATGGTTATGAAAAACGATATGGTTTATTCTATGTCGATTTTGAGACTCAAGAACGTTATCCTA
AGAAAAGTGCTTATTGGTACAAAGAGTTAGCCGAAACAAAAGAAATTAAATAA

Upstream 100 bases:

>100_bases
AGACTCAAGGTGCTGAATATATCAAATTGACACGAGACGGCCAAGCAGCCCTTGATTTCGTTCAACAACAATTTGAAAAT
TAAGAATAGGAGTTTTTCAT

Downstream 100 bases:

>100_bases
TTATAGATATTAAAAGGCCTATCAGTCGAGTGCTGGTAGGTCTTTATTTATAGAAAGAGATAAATTAAACAAGTATATAC
ATATTTATATATTAATCAAT

Product: 6-phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase; PGALase; P-beta-Gal; PBG

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRI
FPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGD
KGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYV
RQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK

Sequences:

>Translated_470_residues
MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRI
FPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGD
KGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYV
RQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK
>Mature_469_residues
TKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIF
PNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEI
GPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHALPTKYPFDPSNPEDVRAAELEDII
HNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDK
GGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYVR
QHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK

Specific function: Can Hydrolyze Salicin, Cellobiose, And Probably Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family

Homologues:

Organism=Homo sapiens, GI110681710, Length=480, Percent_Identity=30.2083333333333, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI13273313, Length=479, Percent_Identity=31.3152400835073, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI32481206, Length=484, Percent_Identity=30.3719008264463, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI24497614, Length=492, Percent_Identity=28.6585365853659, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI28376633, Length=201, Percent_Identity=36.81592039801, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1789070, Length=494, Percent_Identity=34.8178137651822, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI2367174, Length=493, Percent_Identity=32.0486815415822, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI2367270, Length=498, Percent_Identity=32.7309236947791, Blast_Score=195, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17552856, Length=495, Percent_Identity=31.3131313131313, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17539390, Length=492, Percent_Identity=29.2682926829268, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI21356577, Length=487, Percent_Identity=28.7474332648871, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LACG_STAA1 (A7X569)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442763.1
- ProteinModelPortal:   A7X569
- SMR:   A7X569
- STRING:   A7X569
- EnsemblBacteria:   EBSTAT00000005034
- GeneID:   5559027
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2173
- eggNOG:   COG2723
- GeneTree:   EBGT00050000024342
- HOGENOM:   HBG316462
- OMA:   FCFKEFS
- ProtClustDB:   PRK13511
- BioCyc:   SAUR418127:SAHV_2173-MONOMER
- HAMAP:   MF_01574
- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928
- Gene3D:   G3DSA:3.20.20.80
- PANTHER:   PTHR10353
- PRINTS:   PR00131
- TIGRFAMs:   TIGR01233

Pfam domain/function: PF00232 Glyco_hydro_1; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.85

Molecular weight: Translated: 54565; Mature: 54434

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: ACT_SITE 160-160 ACT_SITE 375-375

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYNRYPVDL
CCCCCCCHHHCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHCCCCCE
ELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTP
EEHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEIGPIGDGQYLVGKFPPGIKY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEECCCCCCCH
DFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
HHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYM
HHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHH
SDWMRGYDGESEITHNATGDKGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMR
HHHHHCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHH
VVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYVRQHLNVIADAIKDGANVKGY
HHHCCCCCEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
FIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK
HHHHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWYTAEPASDFYNRYPVDL
CCCCCCHHHCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHCCCCCE
ELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAECHKRHVEPFVTLHHFDTP
EEHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNEIGPIGDGQYLVGKFPPGIKY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEEECCCCCCCH
DFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHALPTKYPFDPSNPEDVRAAELEDI
HHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
IHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNITDEDYAILDAAKDLNDFLGINYYM
HHCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHH
SDWMRGYDGESEITHNATGDKGGSKYQLKGVGQREFDVDVPRTDWDWMIYPQGLYDQIMR
HHHHHCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHH
VVKDYPNYHKIYITENGLGYKDEFIESEKTVHDDARIDYVRQHLNVIADAIKDGANVKGY
HHHCCCCCEEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
FIWSLMDVFSWSNGYEKRYGLFYVDFETQERYPKKSAYWYKELAETKEIK
HHHHHHHHHHCCCCCCCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA